scholarly journals The Impact of Electromagnetic Radiation of Different Parameters on Platelet Oxygen Metabolism – In Vitro Studies

2015 ◽  
Vol 24 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Małgorzata Lewicka ◽  
Gabriela Henrykowska ◽  
Krzysztof Pacholski ◽  
Artur Szczęsny ◽  
Maria Dziedziczak-Buczyńska ◽  
...  
Keyword(s):  
Electromagnetic Radiation ◽  
Oxygen Metabolism ◽  
In Vitro Studies ◽  
The Impact

scholarly journals The effect of electromagnetic radiation emitted by display screens on cell oxygen metabolism – in vitro studies

2015 ◽  
Vol 6 ◽  
pp. 1330-1339 ◽  
Author(s):  
Małgorzata Lewicka ◽  
Gabriela A. Henrykowska ◽  
Krzysztof Pacholski ◽  
Janusz Śmigielski ◽  
Maciej Rutkowski ◽  
...  
Keyword(s):  
Electromagnetic Radiation ◽  
Oxygen Metabolism ◽  
In Vitro Studies

scholarly journals Time-dependent and force-dependent vasoreactivity of isolated human umbilical arteries

2020 ◽  
Vol 51 (3) ◽  
pp. 134-140
Author(s):  
Đorđe Đukanović ◽  
Milica Gajić ◽  
Ranko Škrbić
Keyword(s):  
Incubation Period ◽  
Cold Storage ◽  
Dose Response ◽  
In Vitro Studies ◽  
Time Dependent ◽  
Response Curves ◽  
Experimental Conditions ◽  
Umbilical Arteries ◽  
The Impact

Background/Aim: There have been different experimental conditions for in vitro studies on human umbilical arteries (HUA) in tissue bath system. This diversity was mainly reflected in variables such as stretching tension, incubation period and initial constriction challenging with potassium (KCl). The aim of the study was to establish optimal experimental conditions which will provide better responsiveness of HUA preparations, as well as to examine the impact of 24 h cold storage on viability and responsiveness of HUA to KCl and serotonin. Methods: The KCl-induced constrictions at different stretching tensions (0.5 g, 1.0 g, 2.0 g, 4.0 g), incubation times (30 min, 60 min, 120 min), and after multiple initial constriction challenging were compared. Dose response curves for serotonin were obtained under different conditions (1.0 g and 60 min vs. 2.0 g and 120 min). The influence of 24 h cold storage on KCland serotonininduced vasoconstriction of HUA preparations was examined as well. Results: The strongest constrictions induced by serotonin or KCl were obtained when preparations were adjusted at 2.0 g and incubated for 120 min. The KCl-induced constrictions observed after 120 min were statistically higher (p < 0.05) when preparations were challenged three times (30 min, 60 min, 120 min), compared to those challenged only once. The preparations that were stored at 4 ⁰C for 24 h showed significantly stronger serotonin-induced constrictions (p < 0.01). The cold storage had no influence on KCl-induced constriction. Conclusion: For performing in vitro studies on HUA preparations in tissue bath, we propose stretching tension of 2.0 g, incubation period of 120 min and multiple initial constriction challenging with KCl as optimal experimental condition. We also showed that HUA preparations retained functional viability even after 24 h of cold storage.

scholarly journals THE IMPACT OF ELECTROMAGNETIC RADIATION OF MOBILE PHONE FOR LYMPHOCYTE ACTIVATION IN VITRO

2019 ◽  
Vol 96 (10) ◽  
pp. 965-970 ◽  
Author(s):  
Maria S. Blyakher ◽  
E. A. Tulskaya ◽  
I. V. Kapustin ◽  
I. M. Fedorova ◽  
T. K. Lopatina ◽  
...  
Keyword(s):  
Electromagnetic Radiation ◽  
Mobile Phone ◽  
Blood Cells ◽  
Cytokine Production ◽  
Venous Blood ◽  
Peripheral Blood Lymphocytes ◽  
Mean Values ◽  
Healthy Donors ◽  
The Impact

The character of the influence of the electromagnetic radiation (EMR) of mobile phone on the activation of lymphocytes in vitro was investigated. This is important, since modern human is exposed to a complex combination of electric and magnetic fields (EMF) of different frequencies. The object of the study were whole venous blood and lymphocytes isolated from 21 adult donors (aged of from 20 to 55 years) - 10 were healthy donors and 11 were healthy persons 7 days after their vaccination with meningococcal polysaccharide vaccine. In the study the influence of phone’s EMR on the functional activity of peripheral blood lymphocytes was determined by the flow cytometry method with the use of monoclonal antibodies of Beckman Coulter company (by the identification and calculation the number of basic and activated lymphocyte subpopulations). The changes of cytokines production by blood cells exposed to mobile phone electromagnetic radiation were determined in supernatants by measuring their concentration using EIA kits produced by JSC “Vector-Best” (Russia) and LLC “cytokine” (Russia). The results of the study of the effects of electromagnetic radiation of mobile phone on blood cells revealed changes in the percentage of lymphocytes carrying the early activation marker CD69 significantly to be more frequently and were observed with greater intensity in the group of donors which were vaccinated compared to healthy donors. Under the influence of phone’s EMR mean values of cytokine production determined in the supernatants samples did not changed in both groups, but in the group of healthy donors mean values of cytokines production were 1,5 - 2 times higher than in the group of persons following immunization. The increase or decrease in cytokine production under the influence of phone’s EMR occurred regardless of the initial level of its production in the surveyed donor. The changes of the cytokine production (IFNγ, TNFα, IL-6 and IL-8) by blood cells under the influence of phone’s EMR happen individually; this should be considered when deciding on the presence or absence of phone’s EMR impact on the status of lymphocytes.

scholarly journals ELECTROMAGNETIC FIELD INFLUENCES ON HUMAN HEALTH

2019 ◽  
Vol 33 (1) ◽  
pp. 67-70
Author(s):  
Miodrag Šmelcerović
Keyword(s):  
Electromagnetic Radiation ◽  
Human Health ◽  
Electromagnetic Fields ◽  
New Technologies ◽  
Biological Effects ◽  
Epidemiological Studies ◽  
Human Organism ◽  
Portable Communication ◽  
The Impact

The environment we live in is exposed to the increasing and increasing frequency of electromagnetic radiation in our homes and workplaces. In addition to natural radiation from the sun, radiation sources such as high-voltage transmission lines and powerful radar devices are sources of strong electric and magnetic fields. Increasing the number of portable communication and entertainment devices also increase the human body's exposure to additional electromagnetic radiation. This paper describes the most common effects of low-frequency non-ionizing electromagnetic fields (ELFs), which can cause biological changes, sometimes negative for human health. Different methods and approaches are used to investigate the effect of non-ionizing electromagnetic fields on biological systems. In vitro cell culture studies provide important insights into the underlying mechanisms of biological effects of low radiation levels. It is often not possible to deduce the functional response of a human organism to a particular biological effect. In vivo animal and human studies provide more convincing evidence of possible adverse health effects. There is a problem with the extrapolation of the results obtained from animal experiments to humans. Epidemiological studies provide the most direct information on the risk of adverse effects in humans. However, it is difficult to find good control groups that in all aspects (gender, similar life habits, etc.) fit the exposed groups. Care should be taken in interpreting the results of epidemiological studies, especially if low risk is found, as this may be due to other factors. Epidemiological studies are important for monitoring the impact of new technologies on human health [1].

scholarly journals Myofibroblasts cause heterogeneous Cx43 reduction and are unlikely to be coupled to myocytes in the healing canine infarct

2012 ◽  
Vol 302 (3) ◽  
pp. H790-H800 ◽  
Author(s):  
Jennifer R. Baum ◽  
Biao Long ◽  
Candido Cabo ◽  
Heather S. Duffy
Keyword(s):  
Inflammatory Responses ◽  
Cardiac Fibroblasts ◽  
Cell Communication ◽  
Canine Model ◽  
In Vitro Studies ◽  
Border Zone ◽  
Conduction Abnormalities ◽  
The Impact ◽  
Intact Heart

Following myocardial infarction (MI) inflammatory responses transform cardiac fibroblasts to myofibroblasts, which in vitro studies show form heterocellular gap junctions with cardiac myocytes via Connexin43 (Cx43). The ability to form heterocellular junctions in the intact heart and the impact of these junctions on propagation is unclear. We used a canine model of MI and characterized the distribution and quantity of myofibroblasts in surviving epicardial cells [epicardial border zone (EBZ)]. We found a significant increase in myofibroblasts within the EBZ and no gap junction plaques between myofibroblasts and myocytes. Because myofibroblasts produce IL-1β, which downregulates Cx43, we asked whether myofibroblast proliferation causes loss of Cx43 near myofibroblast clusters. In vitro studies showed that IL-1β caused loss of Cx43 and reduced coupling. Western blot showed a significant increase of IL-1β in the EBZ, and immunohistochemistry showed a loss of Cx43 in regions of myofibroblasts in the intact heart. Additionally, dye studies in intact heart showed no coupling between myocytes and myofibroblasts. To quantify the effect of myofibroblasts on propagation we used a two-dimensional subcellular computer model of the EBZ, which showed that heterogeneities in myofibroblast density lead to conduction abnormalities. In conclusion, an increase of myofibroblasts in the infarcted heart causes heterogeneous Cx43 levels, possibly as a result of the release of IL-1β and decreased cell-cell communication, which leads to conduction abnormalities following MI.

The effect of 50 hz magnetic field of different shape on oxygen metabolism in blood platelets: in vitro studies

2009 ◽  
Vol 22 (3) ◽  
Author(s):  
Gabriela Henrykowska ◽  
Wojciech Jankowski ◽  
Krzysztof Pacholski ◽  
Małgorzata Lewicka ◽  
Janusz Śmigielski ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Blood Platelets ◽  
Oxygen Metabolism ◽  
In Vitro Studies

scholarly journals The European Hot Spot of B[a]P and PM2.5 Exposure—The Ostrava Region, Czech Republic: Health Research Results

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Radim J. Sram ◽  
Miroslav Dostal ◽  
Helena Libalova ◽  
Pavel Rossner ◽  
Andrea Rossnerova ◽  
...  
Keyword(s):  
Czech Republic ◽  
Molecular Epidemiology ◽  
Asthma Bronchiale ◽  
Hot Spot ◽  
Industrial Area ◽  
Control Area ◽  
In Vitro Studies ◽  
Limit Values ◽  
The Impact

The Ostrava Region in the Czech Republic is a heavily polluted industrial area. Concentrations of PM10, PM2.5, and benzo[a]pyrene (B[a]P) significantly exceed limit values. To investigate the impact of these levels on human health, epidemiological, molecular epidemiology, and in vitro studies were done in 2008–2011. Morbidity of children was followed in 10 pediatric districts. In the most polluted district, children suffered higher incidence of acute respiratory diseases in the first year of life, and higher prevalence of asthma bronchiale. Gene expression was studied in children from Ostrava and from a control rural area. Genes specific to asthma bronchiale differed, suggesting a different molecular phenotype in children in the polluted region compared to children in the control area. A molecular epidemiology study showed adverse effect of the Ostrava exposures, but also an increased expression of XRCC5, which probably protects these exposed subjects against the degree of genetic damage that would otherwise be expected. In vitro studies clearly related concentration of B[a]P from PM2.5 extracts to induced PAH-DNA adducts. These studies clearly demonstrate that under the present local environmental conditions, the health of the population is severely impaired and will likely remain so for a significant period of time.

scholarly journals The antioxidant effects of melatonin in blood platelets during exposure to electromagnetic radiation – an in vitro study

2021 ◽  
Vol 75 (1) ◽  
pp. 889-895
Author(s):  
Małgorzata Lewicka ◽  
Magdalena Zawadzka ◽  
Gabriela Henrykowska ◽  
Maciej Rutkowski ◽  
Andrzej Buczyński
Keyword(s):  
Oxidative Stress ◽  
Electromagnetic Radiation ◽  
Blood Platelets ◽  
In Vitro Study ◽  
Oxygen Metabolism ◽  
Protective Role ◽  
Negative Effect ◽  
Antioxidant Effects

Abstract Objectives The article presents the results of an in vitro study aimed at identifying changes in parameters of oxidative stress – concentration of malondialdehyde (MDA), enzymatic activity of superoxide dismutase (SOD-1) and protective antioxidant role of melatonin (MLT) during the exposure of blood platelets to electromagnetic radiation (EMR) emitted by monitors. Methods Platelets were exposed to an EMR for 30- and 60 min. generated by monitors (1 kHz frequency, 220 V/m intensity). In each sample the level of SOD-1 activity and concentration of MDA were determined. Results The MDA concentration increased significantly after 30-and 60-min. irradiation, as compared to control values (2.53 vs 1.36; 3.64 vs 1.36 nmol/109 blood platelets) and after the addition of MLT it decreased (2.53 vs 1.55; 3.64 vs 1.12 nmol/109 blood platelets). The activity of SOD-1 increased significantly compared to control values after 30 min. and 60 min. of exposure to EMR (1.97vs 0.75; 2.08 vs 0.75 U/g of protein), and significantly decreased after the addition of MLT only in samples exposed for 60 min. (2.08 vs 0.95 U/g of protein). Discussion The results demonstrated the possibly negative effect of EMR on oxygen metabolism of blood platelets and indicated a possible protective role of melatonin in this process.

Sign in / Sign up

Export Citation Format

Share Document