scholarly journals EXPERIMENTAL RESULTS OF RAT HYALINE CARTILAGE SURFACE DEFECT REPLACEMENT WITH A CELL ENGINEERING STRUCTURE

2018 ◽  
pp. 13 ◽  
Author(s):  
Михаил Сергеевич Божокин ◽  
Cветлана Анатольевна Божкова ◽  
Георгий Иванович Нетылько ◽  
Дмитрий Георгиевич Наконечный ◽  
Миральда Ивановна Блинова ◽  
...  
Keyword(s):  
Surface Defect ◽  
Hyaline Cartilage ◽  
Experimental Results ◽  
Cell Engineering ◽  
Cartilage Surface ◽  
Engineering Structure ◽  
A Cell

Experimental Replacement of the Surface Defect of Rat Hyaline Cartilage by a Cell-Engineered Construct

2021 ◽  
Author(s):  
M. S. Bozhokin ◽  
S. A. Bozhkova ◽  
G. I. Netylko ◽  
D. G. Nakonechny ◽  
Y. A. Nashchekina ◽  
...  
Keyword(s):  
Surface Defect ◽  
Hyaline Cartilage ◽  
A Cell ◽  
Experimental Replacement

An Ultrafast Vitrification Method for Cell Cryopreservation

2017 ◽  
Vol 140 (1) ◽  
Author(s):  
Fengmin Su ◽  
Nannan Zhao ◽  
Yangbo Deng ◽  
Hongbin Ma
Keyword(s):  
Thin Film ◽  
Cooling Rate ◽  
Experimental Results ◽  
Low Concentration ◽  
Temperature Range ◽  
Thin Film Evaporation ◽  
Film Evaporation ◽  
A Cell ◽  
Cryoprotective Solution ◽  
Ultrafast Cooling

Ultrafast cooling is the key to successful cell vitrification cryopreservation of lower concentration cryoprotective solution. This research develops a cell cryopreservation methodology which utilizes thin film evaporation and achieves vitrification of relatively low concentration cryoprotectant with an ultrafast cooling rate. Experimental results show that the average cooling rate of dimethylsulfoxide (DMSO) cryoprotective solution reaches 150,000 °C/min in a temperature range from 10 °C to −180 °C. The ultrafast cooling rate can remarkably improve the vitrification tendencies of the cryoprotective solution. This methodology opens the possibility for more successful cell vitrification cryopreservation.

scholarly journals A cell‐free approach with a supporting biomaterial in the form of dispersed microspheres induces hyaline cartilage formation in a rabbit knee model

2020 ◽  
Vol 108 (4) ◽  
pp. 1428-1438 ◽  
Author(s):  
Javier Zurriaga Carda ◽  
Maria L. Lastra ◽  
Carmen M. Antolinos‐Turpin ◽  
Rosa M. Morales‐Román ◽  
María Sancho‐Tello ◽  
...  
Keyword(s):  
Hyaline Cartilage ◽  
Cartilage Formation ◽  
Knee Model ◽  
Rabbit Knee ◽  
A Cell

Research studies of aging changes of hyaline cartilage surface by using Raman-scattering spectroscopy

2017 ◽  
Author(s):  
Elena V. Timchenko ◽  
Pavel E. Timchenko ◽  
Larisa T. Volova ◽  
Dmitry A. Dolgyshkin ◽  
Anna S. Tyumchenkova ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Hyaline Cartilage ◽  
Cartilage Surface ◽  
Raman Scattering Spectroscopy ◽  
Research Studies

An Ultra Fast Cooling Method for Cell Vitrification Cryopreservation Utilizing Thin Film Evaporation

2016 ◽  
Author(s):  
Fengmin Su ◽  
Nannan Zhao ◽  
Yangbo Deng ◽  
Bohan Tian ◽  
Chunfeng Mu ◽  
...  
Keyword(s):  
Thin Film ◽  
Cooling Rate ◽  
Experimental Results ◽  
Thin Film Evaporation ◽  
Ultra Fast Cooling ◽  
Film Evaporation ◽  
Cooling Method ◽  
A Cell ◽  
Fast Cooling ◽  
Cryoprotective Solution

Ultra-fast cooling is the key to successful cell vitrification cryopreservation of lower concentration cryoprotective solution. This research develops a cell cryopreservation methodology which utilizes thin film evaporation and achieves vitrification of relatively low concentration cryoprotectant with an ultra-fast cooling rate. Experimental results show that the average cooling rate of dimenthylsulphoxide cryoprotective solution reaches 150,000°C/min in a temperature range from 10°C to −180°C. The ultra-fast cooling rate can remarkably improve the vitrification tendencies of the cryoprotective solution. This methodology opens the possibility for more successful cell vitrification cryopreservation.

Characterization of Surface Defect Structure by Low Energy Electron Diffraction

1984 ◽  
Vol 41 ◽  
Author(s):  
J. F. Wendelken ◽  
G. -C. Wang ◽  
J. M. Pimbley ◽  
T. -M. Lu
Keyword(s):  
Electron Beam ◽  
Electron Diffraction ◽  
Surface Defect ◽  
Low Energy ◽  
Experimental Results ◽  
Energy Electron ◽  
Low Energy Electron Diffraction ◽  
Low Energy Electron

AbstractLow energy electron diffraction is a surface sensitive tool which is most widely used for the determination of surface symmetries and equilibrium atomic positions. Experimental and theoretical advances made in the past five years make it possible now to use LEED also for the characterization of a wide variety of surface defect structures. In this paper a variety of experimental results involving analysis of diffracted electron beam shapes as a function of primary electron beam energy, adsorbate coverage, crystal tem-perature and ordering time are presented. These experimental results coupled with kinematic theory, allow the determination of step density, size and shape of reconstruction domains and overlayer islands, island size distribution in an overlayer during growth, and the mode of growth.

scholarly journals Hyaline cartilage differentiation of fibroblasts in regeneration and regenerative medicine

Development ◽  
2022 ◽  
Author(s):  
Ling Yu ◽  
Yu-Lieh Lin ◽  
Mingquan Yan ◽  
Tao Li ◽  
Emily Y. Wu ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Regenerative Medicine ◽  
Hyaline Cartilage ◽  
Fibroblast Cell ◽  
Similar Response ◽  
Articular Chondrocyte ◽  
Joint Injury ◽  
A Cell ◽  
Culture Strategy

Amputation injuries in mammals are typically non-regenerative, however joint regeneration is stimulated by BMP9 treatment (Yu et al., 2019) indicating the presence of latent articular chondrocyte progenitor cells. BMP9 induces a battery of chondrogenic genes in vivo, and a similar response is observed in cultures of amputation wound cells. Extended cultures of BMP9 treated cells results in differentiation of hyaline cartilage and single cell RNAseq analysis identified wound fibroblasts as BMP9 responsive. This culture model was used to identify a BMP9 responsive adult fibroblast cell line and a culture strategy was developed to engineer hyaline cartilage for engraftment into an acutely damaged joint. Transplanted hyaline cartilage survived engraftment and maintained a hyaline cartilage phenotype but did not form mature articular cartilage. In addition, individual hypertrophic chondrocytes were identified in some samples indicating that the acute joint injury site can promote osteogenic progression of engrafted hyaline cartilage. The findings identify fibroblasts as a cell source for engineering articular cartilage and establishes a novel experimental strategy that bridges the gap between regeneration biology and regenerative medicine.

scholarly journals THE EFFECTS OF CERTAIN HEAVY METALS ON RESPIRATION

1926 ◽  
Vol 9 (4) ◽  
pp. 575-601 ◽  
Author(s):  
S. F. Cook
Keyword(s):  
Heavy Metals ◽  
Carbon Dioxide ◽  
Aspergillus Niger ◽  
Temperature Coefficient ◽  
Active Compound ◽  
Carbon Dioxide Production ◽  
Experimental Results ◽  
Toxic Action ◽  
Chemical Combination ◽  
A Cell

1. The effect of the heavy metals on the respiration of Aspergillus niger is to cause the rate of carbon dioxide production to decrease from the first or to increase and subsequently diminish. 2. The speed of the toxic action varies as a constant power of the concentration. 3. The temperature coefficient of the toxic action is between 1.5 and 2. 4. An hypothesis is advanced to account for the action of the heavy metals, by means of which the experimental results may be accounted for. It is assumed that the metal is activated by a chemical combination with a cell constituent. This active compound alters the velocity constants of the normal respiratory reactions, and thus causes the observed changes in the rate of carbon dioxide production.

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