scholarly journals Age-related NADPH-diaphorase positive bodies in the lumbosacral spinal cord of aged rats

2006 ◽  
Vol 69 (5) ◽  
pp. 297-310 ◽  
Author(s):  
Huibing Tan ◽  
Jianwen He ◽  
Songyan Wang ◽  
Kazuho Hirata ◽  
Zhengwei Yang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Nadph Diaphorase ◽  
Aged Rats ◽  
Lumbosacral Spinal Cord ◽  
Age Related

Localization of NADPH diaphorase in the lumbosacral spinal cord and dorsal root ganglia of the cat

1994 ◽  
Vol 339 (1) ◽  
pp. 62-75 ◽  
Author(s):  
Margaret A. Vizzard ◽  
Susan L. Erdman ◽  
Vickie L. Erickson ◽  
Robert J. Stewart ◽  
James R. Roppolo ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Nadph Diaphorase ◽  
Lumbosacral Spinal Cord

Age-related change in the neuropeptide Y and NADPH-diaphorase-positive neurons in the cerebral cortex and striatum of aged rats

1997 ◽  
Vol 223 (3) ◽  
pp. 157-160 ◽  
Author(s):  
Youngbuhm Huh ◽  
Changok Kim ◽  
Wonkyu Lee ◽  
Junghye Kim ◽  
Heekyung Ahn
Keyword(s):  
Cerebral Cortex ◽  
Neuropeptide Y ◽  
Nadph Diaphorase ◽  
Aged Rats ◽  
Age Related

scholarly journals Spacing spheroids, curl-up and coiled neuritic plexus of the sacral spinal cord of aged dog: Hypothesis based on continued NADPH diaphorase histological observations

2020 ◽  
Author(s):  
Yunge Jia ◽  
Yinhua Li ◽  
Wei Hou ◽  
Huibing Tan
Keyword(s):  
Spinal Cord ◽  
Nadph Diaphorase ◽  
Vacuolar Degeneration ◽  
Horizontal Section ◽  
Lumbosacral Spinal Cord ◽  
Pelvic Organs ◽  
Backward Reasoning ◽  
And Function ◽  
Aged Dog ◽  
Sacral Spinal Cord

ABSTRACTNADPH diaphorase (N-d) neurons distribute in spinal cord and function for visceral sensation and autonomic regulation. N-d positive neurons innervate pelvic organs. In previous investigation, we report that aging-related N-d body (ANB) in the lumbosacral spinal cord in aged rat and megaloneurite in the sacral spinal cord in aged dog. This article was a continued data report of aging-related N-d alterations in aged dog. N-d positivity in aged spinal cord has revealed a certain of morphological profiles in the spinal cord of several species. However, we still found some denoted N-d neurodegenerative changes that we failed to notice in our previous studies when re-examination of the sacral spinal cord of aged dog. In the horizontal section, spacing spheroids in the superficial laminae of the dorsal horn, curl-up and coiled neurites in the intermediate zone were detected in the sacral spinal cord. The ANB and vacuolar neurite were also detected. Vacuolar degeneration also occurred in the dorsal ganglia at the sacral segment. The curl-up and coiled neurites did not occur in the lumbothoracic segment, but the ANB and vacuolar neurite were scatteringly detected in in the lumbothoracic segment of aged dog. The results suggested that the N-d sensory inputs interrupted and disconnected with integration of autonomic centers and output circuits of regulating urogenital organs during the aging. These specialized profiles were speculated that the N-d neurite deterioration of visceral sensory circuit implicated dysfunction of pelvic organs in the aging. Megaloneurite and fiber dilation may make backward reasoning to N-d fiber architecture under normal condition.

scholarly journals A comparative assessment of aging-related NADPH diaphorase positivity in the spinal cord and medullary oblongata between pigeon and murine

2019 ◽  
Author(s):  
Yunge Jia ◽  
Wei Hou ◽  
Yinhua Li ◽  
Xiaoxin Wen ◽  
Chenxu Rao ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Morphological Changes ◽  
Cell Types ◽  
Central Canal ◽  
Ventral Horn ◽  
Nadph Diaphorase ◽  
Aged Rats ◽  
Column Of Terni ◽  
The Central Nervous System ◽  
Rats And Mice

AbstractNADPH diaphorase (N-d) positive neurons has been examined in many animals. N-d neurodegenerative neurites were detected in some animal models. However, detailed information of N-d positivity and aging related changes was still lack in the spinal cord and medulla oblongata of pigeons. In this study, we evaluated the N-d positivity and aging alterations in the spinal cord and medullary oblongata of the pigeon compared with rat and mouse. In pigeons, N-d neurons were more numerous in the dorsal horn, around the central canal and in the column of Terni in the thoracic and lumbar segments and scattered neurons occurred in the ventral horn of spinal segments. N-d neurons also occurred in the white matter of spinal cord. Morphometrical analysis demonstrated in the lumbosacral, cervical and thoracic regions. Compared with young pigeons, the size of N-d soma was significantly altered in aged pigeons. Meanwhile, the dramatic morphological changes occurred in the lumbar to sacral segments. The most important findings of this study were aging-related N-d positive bodies (ANB) in aged pigeons, mainly in the nucleus cuneatus externus (CuE), occasionally in the nuclei gracilis et cuneatus. ANBs were identified in the gracile nuclei in spinal cord in the aged rats and mice. ANBs were also detected in the CuE spinal nucleus in the aged rats. Immunohistochemistry also showed that the aging changes occurred in the cell types and neuropeptides in aged animals. The results suggested the weak inflammation and neuronal dysfunction in the spinal cord in aged pigeons. Our results suggested that the ANB could be considered as aging marker in the central nervous system.

Novel Morphological Alteration and Neural Pathway of NADPH Diaphorase Positivity in the Spinal Cord with Disc Herniation of Aged Dog: A Case Report

2020 ◽  
Author(s):  
Yinhua Li ◽  
Yunge Jia ◽  
Wei Hou ◽  
Huibing Tan
Keyword(s):  
Spinal Cord ◽  
Disc Herniation ◽  
Nadph Diaphorase ◽  
Spinal Trigeminal Nucleus ◽  
Trigeminal Nucleus ◽  
Neural Pathway ◽  
Lumbosacral Spinal Cord ◽  
Spinal Disc ◽  
Collateral Pathway ◽  
Spinal Disc Herniation

Neuronal lesion or injury is a traditional approach to investigate neural circuit. Is any new neural pathway or new neurodegeneration related central nerve system injury? Spinal disc herniation can cause the spinal cord injury. However, the histological examination is still lack. It happened that a case of spinal disc herniation of a 10-year old dog was examined with NADPH diaphorase (N-d) histology. We did not find the N-d neurodegenerative aberrant in the tissue of the mid-rostral lumber segment besides the metamorphoses by the compression of the disc herniation. However, the severe neuropathological changes majorly occurred in the lumbosacral spinal cord. We found more diverse neurodegenerative alterations: the aging-related N-d body (ANB), megaloneurite and N-d homogeneous formazan globule in the lumbosacral spinal cord. We also found that a new circuit pathway (intermedial collateral pathway) showed by a megaloneurite between the lateral collateral pathway and the medial collateral pathway. The enormous notch caused by spinal disc herniation located at the mid-rostral lumber segments. The aging-related neurodegeneration occurred the specific lumbosacral segments. The homogeneous formazan globule was round or oval homogeneous N-d positivity which distributed in the gray matter and dorsal column. In the medulla oblongata, ANBs were revealed in the gracile nucleus, nucleus reticularis lateralis (ventrolateral spinal trigeminal nucleus) and middle of the spinal trigeminal nucleus.

scholarly journals Dendranthema zawadskii var. lucidum (Nakai) J.H. Park Extract Inhibits Cellular Senescence in Human Dermal Fibroblasts and Aging-Related Inflammation in Rats

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 801
Author(s):  
Jehun Choi ◽  
Gwi-Yeong Jang ◽  
Jeonghoon Lee ◽  
Hae-Young Chung ◽  
Hyung-Jun Noh ◽  
...  
Keyword(s):  
Cellular Senescence ◽  
Cell Cycle Progression ◽  
Inflammatory Responses ◽  
Dermal Fibroblast ◽  
Dermal Fibroblasts ◽  
Dependent Manner ◽  
Aged Rats ◽  
Age Related ◽  
Dendranthema Zawadskii ◽  
Beta Galactosidase

Senescence is the phenomenon by which physiological functions of organisms degenerate with time. Cellular senescence is marked by an inhibition of cell cycle progression. Beta-galactosidase accumulates in the lysosomes of aged cells. In this study, human dermal fibroblast cells (HDFs) were treated with 0.5 μM doxorubicin for 4 h to induce cellular senescence. Senescence-associated beta-galactosidase (SA-β-gal) activity was then measured 72 h after treatment with aerial parts of Dendranthema zawadskii var. lucidum (Nakai) J.H. Park (DZ) extract. Treatment with DZ extract significantly decreased SA-β-gal activity in a dose-dependent manner in HDFs. Additionally, DZ extract treatment reduced age-related oxidative stress and inflammation in the aortas of aged rats. The reactive oxygen species (ROS) levels in aortas of aged control rats were higher than those in young rats. However, DZ extract-fed aged rats showed significantly lower ROS levels than the aged control rats. When the aged rats were treated with DZ extract at either 0.2 or 1.0 mg∙kg−1∙day−1, NF-κB levels in aorta tissue decreased significantly compared to those in aorta tissue of the aged control rats without DZ treatment. In addition, DZ extract-fed aged rat aortas showed significant reductions in expression of iNOS and COX-2 induced by NF-κB translocation. Therefore, these results suggest that DZ effectively inhibited senescence-related NF-κB activation and inflammation. DZ extract may have a role in the prevention of the vascular inflammatory responses that occur during vascular aging.

scholarly journals Rostrocaudal Distribution of the C-Fos-Immunopositive Spinal Network Defined by Muscle Activity during Locomotion

2021 ◽  
Vol 11 (1) ◽  
pp. 69
Author(s):  
Natalia Merkulyeva ◽  
Vsevolod Lyakhovetskii ◽  
Aleksandr Veshchitskii ◽  
Oleg Gorskii ◽  
Pavel Musienko
Keyword(s):  
Spinal Cord ◽  
Locomotor Activity ◽  
Control Systems ◽  
Muscle Activity ◽  
Knee Extensors ◽  
Emg Activity ◽  
Lumbosacral Spinal Cord ◽  
Adductor Muscles ◽  
Spinal Network ◽  
Hip Flexor

The optimization of multisystem neurorehabilitation protocols including electrical spinal cord stimulation and multi-directional tasks training require understanding of underlying circuits mechanisms and distribution of the neuronal network over the spinal cord. In this study we compared the locomotor activity during forward and backward stepping in eighteen adult decerebrated cats. Interneuronal spinal networks responsible for forward and backward stepping were visualized using the C-Fos technique. A bi-modal rostrocaudal distribution of C-Fos-immunopositive neurons over the lumbosacral spinal cord (peaks in the L4/L5 and L6/S1 segments) was revealed. These patterns were compared with motoneuronal pools using Vanderhorst and Holstege scheme; the location of the first peak was correspondent to the motoneurons of the hip flexors and knee extensors, an inter-peak drop was presumably attributed to the motoneurons controlling the adductor muscles. Both were better expressed in cats stepping forward and in parallel, electromyographic (EMG) activity of the hip flexor and knee extensors was higher, while EMG activity of the adductor was lower, during this locomotor mode. On the basis of the present data, which showed greater activity of the adductor muscles and the attributed interneuronal spinal network during backward stepping and according with data about greater demands on postural control systems during backward locomotion, we suppose that the locomotor networks for movements in opposite directions are at least partially different.

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