scholarly journals Selective expression of L-serine synthetic enzyme 3PGDH in Schwann cells, perineuronal glia, and endoneurial fibroblasts along rat sciatic nerves and its upregulation after crush injury.

2003 ◽  
Vol 66 (5) ◽  
pp. 429-436 ◽  
Author(s):  
Noboru Yamashita ◽  
Kazuhisa Sakai ◽  
Shigeki Furuya ◽  
Masahiko Watanabe
Keyword(s):  
Schwann Cells ◽  
Crush Injury ◽  
Selective Expression ◽  
Sciatic Nerves

scholarly journals Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Petr Dubový ◽  
Ilona Klusáková ◽  
Ivana Hradilová Svíženská
Keyword(s):  
Schwann Cells ◽  
Nerve Injury ◽  
Inflammatory Cytokines ◽  
Close Contact ◽  
Crush Injury ◽  
Sciatic Nerve Crush ◽  
Nerve Crush ◽  
Sciatic Nerves ◽  
Anti Inflammatory ◽  
Nerve Crush Injury

Activated Schwann cells distal to nerve injury upregulate inflammatory mediators, including cytokines. The goal of the present study was to investigate expression of proinflammatory (IL-1β, TNFα) and anti-inflammatory cytokines (IL-4, IL-10) in activated Schwann cells in relation to growing axons distal to crush injury of rat sciatic nerves. Seven days from sciatic nerve crush, transverse cryostat sections were cut 5 mm distal to lesion and incubated for double immunostaining to indicate Schwann cells (GFAP or S100b) and individual investigated cytokines or to demonstrate growing axons (GAP43). The Schwann cells of naïve sciatic nerves and those removed from sham-operated rats displayed similar weak immunoreactivity for the investigated cytokines. In contrast, increased intensity of cytokine immunofluorescence was found in Schwann cells distal to crush lesion. The cytokine-positive Schwann cells were found in close contact with growing axons detected by immunostaining for GAP43. The results of immunohistochemical analysis distal to nerve crush injury suggest that inflammatory profiling of Schwann cells including upregulation of both pro- and anti-inflammatory cytokines does not prevent growth of axons distal to nerve crush injury.

scholarly journals The dynamic changes of main cell types in the microenvironment of sciatic nerves following sciatic nerve injury and the influence of let-7 on their distribution

RSC Advances ◽  
2018 ◽  
Vol 8 (72) ◽  
pp. 41181-41191 ◽  
Author(s):  
Tianmei Qian ◽  
Pan Wang ◽  
Qianqian Chen ◽  
Sheng Yi ◽  
Qianyan Liu ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Nerve Injury ◽  
Cell Types ◽  
Sciatic Nerve Injury ◽  
Dynamic Changes ◽  
Main Cell ◽  
Sciatic Nerves

Schwann cells (SCs), fibroblasts and macrophages are the main cells in the peripheral nerve stumps.

scholarly journals Knockdown of Dock7 <i>in vivo</i> specifically affects myelination by Schwann cells and increases myelin thickness in sciatic nerves without affecting axon thickness

2012 ◽  
Vol 02 (03) ◽  
pp. 210-216 ◽  
Author(s):  
Tomohiro Torii ◽  
Yuki Miyamoto ◽  
Motoshi Nagao ◽  
Naoko Onami ◽  
Hideki Tsumura ◽  
...  
Keyword(s):  
Schwann Cells ◽  
Myelin Thickness ◽  
Sciatic Nerves

scholarly journals Immunocytochemical localization of S-100b protein in degenerating and regenerating rat sciatic nerves.

1989 ◽  
Vol 37 (4) ◽  
pp. 441-446 ◽  
Author(s):  
A Spreca ◽  
M G Rambotti ◽  
M Rende ◽  
C Saccardi ◽  
M C Aisa ◽  
...  
Keyword(s):  
Protein Content ◽  
Schwann Cells ◽  
Immune Reaction ◽  
Cell Types ◽  
Distal Stump ◽  
Over Expression ◽  
Regeneration Period ◽  
S 100 ◽  
Similar Images ◽  
Sciatic Nerves

We studied the cellular and subcellular distribution of S-100b protein in normal, crushed, and transected rat sciatic nerves by an immunocytochemical procedure. In uninjured nerves, S-100b protein was restricted to the cytoplasm and membranes of Schwann cells, with no reaction product present in the nucleus or in axons. Similar images were seen from the first to the thirtieth day after the crush in activated Schwann cells during the degeneration period, i.e., up to the seventh post-lesion day, and in normal Schwann cells reappearing during the regeneration period, i.e., after the seventh post-lesion day, in the zone of the crush and proximal and distal to it. By the technique employed, there seemed to be no differences in the intensity of the immune reaction product in normal and activated Schwann cells. Also, similar images were seen in the proximal stump of transected nerves. Only a slight S-100b protein immune reaction product could be observed in the rare activated Schwann cells present in the distal stump around the seventh post-lesion day, the majority of cell types being represented by fibroblasts and elongated cells at this stage and thereafter. By immunochemical assays, similar results as those presented here have been reported and interpreted as indicative of the presence of S-100 protein in axons or, alternatively, of axonal control over expression of S-100 protein in Schwann cells. Our immunocytochemical data clearly show that the strong reduction in the S-100 protein content of the distal stump of transected nerves is owing to the paucity of Schwann cells and to the decrease in the S-100 protein content of these cells, rather than to degeneration of axons.

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