scholarly journals The dynamic changes of main cell types in the microenvironment of sciatic nerves following sciatic nerve injury and the influence of let-7 on their distribution

RSC Advances ◽  
2018 ◽  
Vol 8 (72) ◽  
pp. 41181-41191 ◽  
Author(s):  
Tianmei Qian ◽  
Pan Wang ◽  
Qianqian Chen ◽  
Sheng Yi ◽  
Qianyan Liu ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Nerve Injury ◽  
Cell Types ◽  
Sciatic Nerve Injury ◽  
Dynamic Changes ◽  
Main Cell ◽  
Sciatic Nerves

Schwann cells (SCs), fibroblasts and macrophages are the main cells in the peripheral nerve stumps.

Transplantation of Schwann cells in a collagen tube for the repair of large, segmental peripheral nerve defects in rats

2013 ◽  
Vol 119 (3) ◽  
pp. 720-732 ◽  
Author(s):  
Yerko A. Berrocal ◽  
Vania W. Almeida ◽  
Ranjan Gupta ◽  
Allan D. Levi
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Nerve Injury ◽  
Peripheral Nerve Injury ◽  
Fluorescent Protein ◽  
Control Groups ◽  
Myelinated Axons ◽  
Segmental Nerve ◽  
Green Fluorescent

Object Segmental nerve defects pose a daunting clinical challenge, as peripheral nerve injury studies have established that there is a critical nerve gap length for which the distance cannot be successfully bridged with current techniques. Construction of a neural prosthesis filled with Schwann cells (SCs) could provide an alternative treatment to successfully repair these long segmental gaps in the peripheral nervous system. The object of this study was to evaluate the ability of autologous SCs to increase the length at which segmental nerve defects can be bridged using a collagen tube. Methods The authors studied the use of absorbable collagen conduits in combination with autologous SCs (200,000 cells/μl) to promote axonal growth across a critical size defect (13 mm) in the sciatic nerve of male Fischer rats. Control groups were treated with serum only–filled conduits of reversed sciatic nerve autografts. Animals were assessed for survival of the transplanted SCs as well as the quantity of myelinated axons in the proximal, middle, and distal portions of the channel. Results Schwann cell survival was confirmed at 4 and 16 weeks postsurgery by the presence of prelabeled green fluorescent protein–positive SCs within the regenerated cable. The addition of SCs to the nerve guide significantly enhanced the regeneration of myelinated axons from the nerve stump into the proximal (p < 0.001) and middle points (p < 0.01) of the tube at 4 weeks. The regeneration of myelinated axons at 16 weeks was significantly enhanced throughout the entire length of the nerve guide (p < 0.001) as compared with their number in a serum–only filled tube and was similar in number compared with the reversed autograft. Autotomy scores were significantly lower in the animals whose sciatic nerve was repaired with a collagen conduit either without (p < 0.01) or with SCs (p < 0.001) when compared with a reversed autograft. Conclusions The technique of adding SCs to a guidance channel significantly enhanced the gap distance that can be repaired after peripheral nerve injury with long segmental defects and holds promise in humans. Most importantly, this study represents some of the first essential steps in bringing autologous SC-based therapies to the domain of peripheral nerve injuries with long segmental defects.

MIF/CD74 axis participates in inflammatory activation of Schwann cells following sciatic nerve injury

2019 ◽  
Vol 50 (4) ◽  
pp. 355-367 ◽  
Author(s):  
Honghua Song ◽  
Ziwen Zhu ◽  
Yue Zhou ◽  
Nan Du ◽  
Tiancheng Song ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Schwann Cells ◽  
Nerve Injury ◽  
Sciatic Nerve Injury ◽  
Inflammatory Activation

VULNERABILITY OF THE GRADUALLY ELONGATED NERVE TO COMPRESSION INJURY

Hand Surgery ◽  
2001 ◽  
Vol 06 (01) ◽  
pp. 29-35 ◽  
Author(s):  
Kazuo Ikeda ◽  
Mitsuteru Yokoyama ◽  
Katsuro Tomita ◽  
Shigenori Tanaka
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Injury ◽  
Control Group ◽  
Severe Damage ◽  
Compression Injury ◽  
Sciatic Nerves

The purpose of this study is to clarify the vulnerability of the gradually elongated peripheral nerve. Rabbit's sciatic nerves were gradually elongated to 30 mm at the rate of 2.0 mm/day and 4.0 mm/day. Immediately after elongation, the sciatic nerve was exposed and compressed for 30 minutes at various forces, 15, 30 and 60 g/0.1 cm2. Immediately after elongation and compression, 2, 4 and 8 weeks after compression, each group was electrophysiologically and histologically estimated — 15 g/0.1 cm2 caused no damage to the control group, neurapraxia to the 2.0 mm/day group, and axonotmesis to the 4.0 mm/day group; 30 g/0.1 cm2 caused neurapraxia to the control group and axonotmesis to the 2.0 mm/day group; 60 g/0.1 cm2 caused axonotmesis to the control group and slowly recovered axonotmesis to the 2.0 mm/day group. This study shows that though mild compression, does not cause nerve injury to the intact nerve, it can sometimes cause severe damage to the gradual elongated nerve.

GDNF mRNA in Schwann cells and DRG satellite cells after chronic sciatic nerve injury

Neuroreport ◽  
1996 ◽  
Vol 7 (4) ◽  
pp. 857-860 ◽  
Author(s):  
H. Hammarberg ◽  
F. Piehl ◽  
S. Cullheim ◽  
J. Fjell ◽  
T Hökfelt ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Schwann Cells ◽  
Nerve Injury ◽  
Satellite Cells ◽  
Sciatic Nerve Injury

Dynamic changes of ICAM-1 expression in peripheral nervous system following sciatic nerve injury

2011 ◽  
Vol 33 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Junling Yang ◽  
Yangyang Gu ◽  
Xiaodong Huang ◽  
Aiguo Shen ◽  
Chun Cheng
Keyword(s):  
Nervous System ◽  
Sciatic Nerve ◽  
Peripheral Nervous System ◽  
Nerve Injury ◽  
Sciatic Nerve Injury ◽  
Dynamic Changes

scholarly journals Decellularized peripheral nerve grafts by a modified protocol for repair of rat sciatic nerve injury

2021 ◽  
Vol 16 (6) ◽  
pp. 1086
Author(s):  
Arash Zaminy ◽  
Sara Sayad-Fathi ◽  
FarshadMoharrami Kasmaie ◽  
Zohreh Jahromi ◽  
Adib Zendedel
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Injury ◽  
Sciatic Nerve Injury ◽  
Nerve Grafts ◽  
Rat Sciatic Nerve ◽  
Peripheral Nerve Grafts

scholarly journals Augmenting Peripheral Nerve Regeneration Using Rat Hair Follicle Stem Cells (rHFSCs) in Rats

2021 ◽  
Author(s):  
Leila Beigom Hejazian ◽  
◽  
Zeinab Akbarnejad ◽  
Fatemeh Moghani Ghoroghi ◽  
Banafshe Esmaeilzade ◽  
...  
Keyword(s):  
Stem Cells ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Hair Follicle ◽  
Nerve Injury ◽  
Peripheral Nerve Injury ◽  
Sciatic Nerve Injury ◽  
Double Labeling ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

Introduction: Nowadays, cell therapy is the most advanced treatment of peripheral nerve injury. The aim of this study was to determine the effects of transplantation of hair follicle stem cells on the regeneration of the sciatic nerve injury in rats. Methods: The bulge region of the rat whisker was isolated and cultured. Morphological and biological features of the cultured bulge cells were observed by light microscopy and immunocytochemistry methods. Percentages of CD34, K15 and Nestin cell markers expression were demonstrated by flow cytometry. Rats were randomly divided into 3 groups: Injury group, epineurium group, and epineurium-with-cell group, that rat hair follicular stem cells (rHFSCs) were injected into the site of nerve cut. HFSCs were labeled with BrdU, and double-labeling immunofluorescence was performed to study survival and differentiation of the grafted cells. After 8 weeks, electrophysiological, histological and immunocytochemical analysis assessments were performed. Results: The results of this study show that rat hair follicle stem cells are suitable for cell culture, proliferation and differentiation. The results suggest that transplantation of rat hair follicle stem cells had the potential capability of regenerating sciatic nerve injury; moreover, evidence of electrophysiology and histology show that Epineurium with cell repair was more effective than the other experimental group (p<0.05). Conclusion: The achieved results propose that hair follicle stem cell would improve axonal growth and functional recovery after peripheral nerve injury.

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