scholarly journals Collagen Fibers, Reticular Fibers and Elastic Fibers. A Comprehensive Understanding from a Morphological Viewpoint.

2002 ◽  
Vol 65 (2) ◽  
pp. 109-126 ◽  
Author(s):  
Tatsuo USHIKI
Keyword(s):  
Collagen Fibers ◽  
Elastic Fibers ◽  
Comprehensive Understanding ◽  
Reticular Fibers

scholarly journals ULTRASTRUCTURAL STUDIES ON THE LYMPHATIC ANCHORING FILAMENTS

1968 ◽  
Vol 36 (1) ◽  
pp. 129-149 ◽  
Author(s):  
L. V. Leak ◽  
J. F. Burke
Keyword(s):  
Connective Tissue ◽  
Extracellular Space ◽  
Collagen Fibers ◽  
Capillary Wall ◽  
Surrounding Tissue ◽  
Elastic Fibers ◽  
Lymphatic Capillary ◽  
Ultrastructural Studies ◽  
Outer Leaflet ◽  
Tissue Area

The fine structure of the lymphatic capillary and the surrounding tissue areas was investigated. Instead of a continuous basal lamina (basement membrane) surrounding the capillary wall, these observations revealed the occurrence of numerous fine filaments that insert on the outer leaflet of the trilaminar unit membrane of the lymphatic endothelium. These filaments appear as individual units, or they are aggregated into bundles that are disposed parallel to the long axis of the lymphatic capillary wall and extend for long distances into the adjoining connective tissue area among the collagen fibers and connective tissue cells. The filaments measure about 100 A in width and have a hollow profile. They exhibit an irregular beaded pattern along their long axis and are densely stained with uranyl and lead. These filaments are similar to the microfibrils of the extracellular space and the filaments observed in the peripheral mantle of the elastic fibers. Infrequently, connections between these various elements are observed, suggesting that the lymphatic anchoring filaments may also contribute to the filamentous units of the extracellular space. It is suggested that these lymphatic anchoring filaments connect the small lymphatics to the surrounding tissues and represent the binding mechansim that is responsible for maintaining the firm attachment of the lymphatic capillary wall to the adjoining collagen fibers and cells of the connective tissue area.

scholarly journals Effect and Repair Mechanism of Nano Ag Sponge Dressing Combined with Gelatin-Bletilla Striata Gum/Salvia Miltiorrhiza on Refractory Orthopedic Wounds

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Chuanjie Jiao ◽  
Ming Deng ◽  
Yonggang Ma ◽  
Geliang Hu
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Healing Rate ◽  
Salvia Miltiorrhiza ◽  
Collagen Fibers ◽  
Hair Follicles ◽  
Elastic Fibers ◽  
Bletilla Striata ◽  
Ctrl Group ◽  
Tgf Β1

Objective. To explore the effect and mechanism of the sponge dressing on the healing of refractory orthopedic wound, and the gelatin-Bletilla striata gum/Salvia miltiorrhiza nano Ag (GBS-Ag) sponge dressing was prepared. Methods. GBS-Ag sponge dressing was prepared by the freeze-drying method. Twenty male SD rats were randomly divided into the control group (Ctrl group) and GBS-Ag group, with 10 rats in each group, and the rats in the two groups were established a model of back wound infection. The Ctrl group was treated with gauze, while the GBS-Ag group was treated with GBS-Ag sponge dressing. Wound healing rate, blood immune indexes, Ag content in each organ, morphological changes of wound, and expression of transforming growth factor-β1 (TGF-β1) in wound transformation were detected in the two groups of rats. Results. The mechanical properties of GBS-Ag sponge dressing were all in line with the standard, and it had good killing effect on the conventional strain after being incubated for 24 hours. Compared with the Ctrl group, the healing rate and lymphocyte percentage in the GBS-Ag group were significantly increased on day 4 and day 10 ( P < 0.05 ), while the total number of white blood cells and the percentage of neutrophils were significantly decreased ( P < 0.05 ). Compared with Ctrl group, the Ag content in liver, spleen, and kidney of rats in the GBS-Ag group was significantly increased ( P < 0.05 ). The histological results showed that the Ctrl group lacked collagen fibers in the dermis, and the angiogenesis was not rich, accompanied by a large number of inflammatory cell infiltration. The epidermal repair of rats in the GBS-Ag group was complete and partially keratinized, the dermis was rich in collagen fibers, with elastic fibers and new blood vessels, inflammatory cells were rare, and new hair follicles and thick-walled blood vessels were also observed. The expression of TGF-β1 protein in the wounds of rats in the GBS-Ag group was higher than that of the Ctrl group. Conclusion. GBS-Ag sponge dressing had multiple effects of sterilization and promoting wound healing, and its mechanism may be related to promoting the TGF-β1 protein expression.

scholarly journals Aging histological changes in the cartilages of the cricoarytenoid joint

2004 ◽  
Vol 19 (2) ◽  
pp. 136-140 ◽  
Author(s):  
Rogério Aparecido Dedivitis ◽  
Márcio Abrahão ◽  
Manoel de Jesus Simões ◽  
Osvaldo Alves Mora ◽  
Onivaldo Cervantes
Keyword(s):  
Hyaline Cartilage ◽  
Muscle Fibers ◽  
Collagen Fibers ◽  
The Other ◽  
Elastic Fibers ◽  
Lamellar Bone ◽  
Fat Tissue ◽  
Hematopoietic Tissue ◽  
Group Iii ◽  
Group I

PURPOSE: Analysis of ossification, bone marrow formation, perichondrium thickness, muscle fibers, collagen fibers and elastic fibers quantities of cricoid and arytenoid cartilages. Design: Correlation morphologic study. METHODS: Twenty-four cricoarytenoid joints were obtained from Caucasian male fresh cadavers divided into three groups with eight specimens in each: group I - adolescents, from 15 to 20; group II - adults, from 25 to 35; and group III - elderly, from 60 to 75. The specimens were stained with H-E; trichrome; Picrosirius; and elastic stain. Histometry was performed for quantitative analysis. Bonferroni Test, Fisher Test and the Variance Analysis were used. RESULTS: At the microscopic analysis, the group I specimens presented typical hyaline cartilage, thin perichondrium, bulky muscle fibers and were surrounded by collagen fibers. In group II, there were ossification in small well defined central areas of four specimens, with lamellar bone tissue. In two of these cases there were central bone cavity full of fat tissue. The other parameters were similar to group I. In group III, most part of hyaline cartilage was replaced by typical lamellar bone tissue with poorly outlined haversian systems. Hematopoietic tissue was noted in six cases and fat tissue in the other two. Perichondrium was thicker. Small muscle fibers were smaller and surrounded by collagen in great quantity. Elastic fibers were present in small quantity in the outer portion of perichondrium in all the groups. CONCLUSIONS: In spite of its lack in adolescence, ossification occurs in cricoid and arytenoid cartilages during adulthood and intensifies with age; bone marrow is formed in ossification tissue with hematopoietic tissue in group III; perichondrium becomes thicker in group III; muscle tissue atrophies in group III and is replaced by collagen fibers; these fibers thicken with age; and elastic fibers is always present in the perichondrium in low quantity.

scholarly journals Morphological analysis of the elastic and collagen fibers in the ram penis

2018 ◽  
Vol 38 (11) ◽  
pp. 2159-2165 ◽  
Author(s):  
Bruno Cesar Schimming ◽  
Gustavo N. Moraes
Keyword(s):  
Extracellular Matrix ◽  
Morphological Analysis ◽  
Sigmoid Flexure ◽  
Tunica Albuginea ◽  
Corpus Cavernosum ◽  
Reproductive Organs ◽  
Collagen Fibers ◽  
Elastic Fibers ◽  
Corpus Spongiosum ◽  
Domestic Ruminants

ABSTRACT: The penis represents the organ of the male’s copulation. It is essential to know the reproductive biology and the morphology of the reproductive organs to increase animal production. In order to contribute to this knowledge and provides information on the ram reproductive morphology, the purpose of this work was to describe the distribution, based on light microscopy, of the collagen and elastic fibers in the ram penis. For that, were collected transverse fragments of the penis (root, sigmoid flexure, body and glans) of seven rams. The specimens were fixed in paraformaldehyde for 24h and destined for the histological routine. The extracellular matrix of the ram penis was composed of collagen and elastic fibers. The penis was enveloped by the tunica albuginea, consisting essentially of collagen fibers, which were arranged in two layers: an outer longitudinal and an inner circular. This tunic emitted septa that penetrated the corpus cavernosum. The elastic fibers appeared transversely and longitudinally in the corpus cavernosum, corpus spongiosum, and next to the neurovascular bundle of the penis. This structure was not different to that reported for other domestic ruminants such as cattle and buffaloes.

Effects of castration on penile extracellular matrix morphology in domestic cats

2017 ◽  
Vol 19 (12) ◽  
pp. 1261-1266 ◽  
Author(s):  
Nathalia CS Borges ◽  
Marco A Pereira-Sampaio ◽  
Vivian Alves Pereira ◽  
Marcelo Abidu-Figueiredo ◽  
Maurício Alves Chagas
Keyword(s):  
Extracellular Matrix ◽  
Tunica Albuginea ◽  
Corpus Cavernosum ◽  
Collagen Fibers ◽  
Elastic Fibers ◽  
Corpus Spongiosum ◽  
Predisposing Factor ◽  
Luminal Area ◽  
Matrix Morphology ◽  
Student’S T

Objectives This study was undertaken to verify the possible modifications caused by hormonal deprivation in the extracellular matrix in the penises of neutered cats. Methods Twenty-seven penises from domestic shorthair cats were collected: 14 samples from intact cats and 13 from neutered cats. Sections were stained with Weigert’s resorcin-fuchsin, hematoxylin and eosin, and picrosirius red. Histomorphometric analysis was performed using light microscopy and image analysis software. The following parameters were analyzed: density of the elastic fibers and collagen fibers in the corpus spongiosum; density of the elastic fibers in the tunica albuginea of the corpus cavernosum and the tunica albuginea of the corpus spongiosum; luminal area of the urethra; area of the corpus spongiosum; area of the corpus cavernosum; and thickness of the urethral epithelium. The data were analyzed using the Shapiro–Wilk test to verify the normal distribution, and groups were compared using Student’s t-test; P <0.05 indicated statistically significant differences. Results Significant differences were observed between intact cats and neutered cats in the density of elastic fibers in the tunica albuginea of the corpus cavernosum (8.13% ± 1.38% vs 3.11% ± 0.66%), tunica albuginea of the corpus spongiosum (4.37% ± 1.08% vs 3.30% ± 1.01%) and corpus spongiosum (6.28% ± 3.03% vs 4.10% ± 2.19%), and density of collagen fibers in the corpus spongiosum (34.11% ± 10.86% vs 44.21% ± 12.72%). Conclusions and relevance The results show a significant decrease in the density of the elastic fibers and a significant increase of the density of the collagen fibers in the corpus spongiosum in neutered animals. This suggests that the compliance of the periurethral region is reduced, and these changes could be a predisposing factor for urethral obstructive disease.

scholarly journals The increased motion of lumbar induces ligamentum flavum hypertrophy in a rat model

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Baojian Wang ◽  
Chunyu Gao ◽  
Ping Zhang ◽  
Wu Sun ◽  
Jingru Zhang ◽  
...  
Keyword(s):  
Rat Model ◽  
Sham Group ◽  
Ligamentum Flavum ◽  
Collagen Fibers ◽  
Male Rats ◽  
Pcr Analysis ◽  
Elastic Fibers ◽  
Sham Operation ◽  
X Rays ◽  
Experimental Group

Abstract Background The purpose of this study was to establish a novel rat model for ligamentum flavum (LF) hypertrophy using increased motion of lumbar and to elucidate the etiology of (LFH). Methods A total number of 30 male rats were used. The increased motion of lumbar was induced by surgical resection of L5/6 posterior elements (n = 15). The other rats underwent a sham operation (n = 15). After 8 weeks, all rats were taken lateral plain X-rays. The LF from L5/6 in both groups were harvested to investigate histological, immunohistological, and real-time PCR analysis. Results According to radiological results, the disc height ratio, flexion ratio, and extension ratio were larger in the rats in the experimental group than that of in the sham group. The HE staining showed that the LF thickness in the experimental group significantly increased in comparison to the sham group. The Masson trichrome staining showed that the ratio of elastic fibers to collagen fibers in experimental group was lower than that in the sham group. The protein and gene expression of TGF-β1, TNF-α, IL-1β, and Col 1 were significantly higher in the experimental group than that in the sham group. Conclusion A relatively safe, simple, and rapid rat model of LFH using increased motion of lumbar was established. The increased motion of lumbar could lead to high expression of inflammatory and fibrotic factors in LF, causing the accumulation of collagen fibers and decreasing of elastic fibers.

scholarly journals Collagen Fibrillogenesis in the Mitral Valve: It’s a Matter of Compliance

2021 ◽  
Vol 8 (8) ◽  
pp. 98
Author(s):  
Richard L. Goodwin ◽  
Arash Kheradvar ◽  
Russell A. Norris ◽  
Robert L. Price ◽  
Jay D. Potts
Keyword(s):  
Mitral Valve ◽  
Triple Helix ◽  
Collagen Fibers ◽  
Elastic Fibers ◽  
Structural Components ◽  
Valvular Interstitial Cells ◽  
Chordae Tendineae ◽  
Valve Function ◽  
Dynamic Structures ◽  
Opening And Closing

Collagen fibers are essential structural components of mitral valve leaflets, their tension apparatus (chordae tendineae), and the associated papillary muscles. Excess or lack of collagen fibers in the extracellular matrix (ECM) in any of these structures can adversely affect mitral valve function. The organization of collagen fibers provides a sophisticated framework that allows for unidirectional blood flow during the precise opening and closing of this vital heart valve. Although numerous ECM molecules are essential for the differentiation, growth, and homeostasis of the mitral valve (e.g., elastic fibers, glycoproteins, and glycans), collagen fibers are key to mitral valve integrity. Besides the inert structural components of the tissues, collagen fibers are dynamic structures that drive outside-to-inside cell signaling, which informs valvular interstitial cells (VICs) present within the tissue environment. Diversity of collagen family members and the closely related collagen-like triple helix-containing proteins found in the mitral valve, will be discussed in addition to how defects in these proteins may lead to valve disease.

scholarly journals Sources of Arterial Vascularization of the Polar Owl’s Kidneys

2021 ◽  
Author(s):  
Marina V. Pervenetskaya ◽  
Lydmila V. Fomenko ◽  
Maksim V. Koshkarev
Keyword(s):  
Connective Tissue ◽  
Collagen Fibers ◽  
Renal Arteries ◽  
Muscle Membrane ◽  
Elastic Fibers ◽  
Elastic Membrane ◽  
High Concentration ◽  
Loose Connective Tissue ◽  
Internal Iliac ◽  
Oil Paints

The sources of vascularization of the kidneys of five polar owl carcasses were studied by filling the vessels with self-hardening plastic Belokril through the femoral artery. High-grade oil paints were added to the monomer to give the vessels the desired color. After the injection, the carcasses were placed in a high concentration caustic soda solution for three days. The resulting corrosion impression was washed under warm water and dried. It was identified that in the lumbar trunk, the main vessel was the descending aorta, from which extra- and intraorganic arteries departed for vascularizing the kidneys. Extraorganic arteries included external and internal iliac, sciatic and middle sacral arteries. Intraorganic arteries included cranial, middle, and caudal renal arteries. Inside the parenchyma of each lobe of the kidney, intraorganic arteries branched in the main type of caudomedial, dorsomedial and lateromedial directions and were subdivided into segmental, interlobular and perilobular arteries and intralobular capillaries. An asymmetry in the branching of the renal arteries was observed. During histological examination, we noted that the renal arteries were lined with endothelium on the inner side and the intima contained endotheliocytes with oval nuclei. Under the endothelial layer were loose collagen fibers running along the middle shell. There was no loose connective tissue between the inner and middle shells, so the subendothelial layer was very weak and there was no internal elastic membrane. The muscle membrane was well developed, with collagen and elastic fibers located between the muscle fibers. The outer shell was represented by loose connective tissue with the presence of arterial and venous vessels. The collagen fibers had a slightly convoluted course. Keywords: birds, polar owl, arteries, kidneys, parenchyma, capillaries, endotheliocytes, intima

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