scholarly journals Transient Expression of Heat Shock Protein (Hsp) 25 in the Dental Pulp and Enamel Organ during Odontogenesis in the Rat Incisor.

2000 ◽  
Vol 63 (4) ◽  
pp. 381-395 ◽  
Author(s):  
Hayato OHSHIMA ◽  
Hisao AJIMA ◽  
Yoshiro KAWANO ◽  
Kayoko NOZAWA-INOUE ◽  
Satoshi WAKISAKA ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Transient Expression ◽  
Dental Pulp ◽  
Enamel Organ ◽  
Rat Incisor

Promotion of the Differentiation of Dental Pulp Stem Cells into Oligodendrocytes by Knockdown of Heat-Shock Protein 27

2022 ◽  
Author(s):  
Shu-Lin Guo ◽  
Chih-Hui Chin ◽  
Chi-Jung Huang ◽  
Chih-Cheng Chien ◽  
Yih-Jing Lee
Keyword(s):  
Stem Cells ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Neurodegenerative Diseases ◽  
Dental Pulp ◽  
Potential Application ◽  
Neural Differentiation ◽  
Heat Shock Protein 27 ◽  
Dental Pulp Stem Cells ◽  
Immunofluorescent Staining

Stem cell-based therapy has been evaluated in many different clinical trials for various diseases. This capability was applied in various neurodegenerative diseases, such as Alzheimer’s disease, which is characterized by synaptic damage accompanied by neuronal loss. Dental pulp stem cells (DPSCs) are mesenchymal stem cells from the oral cavity and have been studied with potential application for regeneration of different tissues. Heat shock protein 27 (HSP27) is known to regulate neurogenesis in the process of neural differentiation of placenta-multipotent stem cells. Here, we hypothesize that HSP27 expression is also critical in neural differentiation of DPSCs. An evaluation of the possible role of HSP27 in differentiation of DPSCs was per-formed by gene knockdown and neural immunofluorescent staining. We found that HSP27 has a role in the differentiation of DPSCs and that knockdown of HSP27 in DPSCs renders cells to oligodendrocyte progenitors. In other words, shHSP27-DPSCs showed NG2-positive immunoreactivity and gave rise to oligodendrocytes or type-2 astrocytes. This neural differentiation of DPSCs may have clinical significance for treatment of patients with neurodegenerative diseases. In conclusion, our data provide an example of oligodendrocyte differentiation of a DPSCs model that may have potential application in human regenerative medicine.

scholarly journals Possible Role of Heat Shock Protein (Hsp) 25 in the Enamel Organ during Amelogenesis in the Rat Molar.

2001 ◽  
Vol 64 (4) ◽  
pp. 369-378 ◽  
Author(s):  
Yumiko OTSUKA ◽  
Kuniko NAKAKURA-OHSHIMA ◽  
Tadashi NODA ◽  
Takeyasu MAEDA ◽  
Hayato OHSHIMA
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Enamel Organ ◽  
Rat Molar

scholarly journals Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element

2001 ◽  
Vol 356 (2) ◽  
pp. 353-359 ◽  
Author(s):  
Alfredo MOLINA ◽  
Emmanuel Di MARTINO ◽  
Joseph A. MARTIAL ◽  
Marc MULLER
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Transient Expression ◽  
Heat Shock Response ◽  
Heat Shock Protein 70 ◽  
Zebrafish Embryos ◽  
Epithelioma Papulosum Cyprini ◽  
Hsp70 Promoter ◽  
Shock Response ◽  
Constitutive Factor

We reported previously that a tilapia (Oreochromis mossambicus) heat shock protein 70 (HSP70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell culture and in microinjected zebrafish embryos. Here we present the first functional analysis of a fish HSP70 promoter, the tiHSP70 promoter. Using transient expression experiments in carp EPC (epithelioma papulosum cyprini) cells and in microinjected zebrafish embryos, we show that a distal heat shock response element (HSE1) at approx. −800 is predominantly responsible for the heat shock response of the tiHSP70 promoter. This element specifically binds an inducible transcription factor, most probably heat shock factor, and a constitutive factor. The constitutive complex is not observed with the non-functional, proximal HSE3 sequence, suggesting that both factors are required for the heat shock response mediated by HSE1.

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