scholarly journals Fine structure of the taste bud in guinea pigs. II. Localization of spot 35 protein, a cerebellar Purkinje cell-specific protein, as revealed by electron-microscopic immunocytochemistry.

1991 ◽  
Vol 54 (1) ◽  
pp. 113-118 ◽  
Author(s):  
Sumio YOSHIE ◽  
Chikashi WAKASUGI ◽  
Yoshimi TERAKI ◽  
Toshihiko IWANAGA ◽  
Tsuneo FUJITA
Keyword(s):  
Fine Structure ◽  
Purkinje Cell ◽  
Guinea Pigs ◽  
Protein A ◽  
Specific Protein ◽  
Taste Bud ◽  
Cerebellar Purkinje Cell ◽  
Electron Microscopic ◽  
Electron Microscopic Immunocytochemistry

scholarly journals Fine structure of the taste bud in guinea pigs. I. Cell characterization and innervation patterns.

1990 ◽  
Vol 53 (1) ◽  
pp. 103-119 ◽  
Author(s):  
Sumio YOSHIE ◽  
Chikashi WAKASUGI ◽  
Yoshimi TERAKI ◽  
Tsuneo FUJITA
Keyword(s):  
Fine Structure ◽  
Guinea Pigs ◽  
Taste Bud ◽  
Cell Characterization ◽  
Innervation Patterns

Fluorescent and Electron Microscopic Studies Suggest That Confronting Cisternae Arise from Nuclear Envelope in Mitotic Cells

1998 ◽  
Vol 4 (S2) ◽  
pp. 1028-1029
Author(s):  
J. R. Palisano ◽  
J. L. Thacker ◽  
C. S. Piromalli ◽  
A. M. Morrison ◽  
J. E. Tate
Keyword(s):  
Monoclonal Antibody ◽  
Nuclear Envelope ◽  
Hela Cells ◽  
Uv Light ◽  
Fluorescent Microscopy ◽  
Protein A ◽  
Specific Protein ◽  
Electron Microscopic ◽  
Lamin B ◽  
Microscopic Studies

The origin and role of confronting cisternae (CC) have been clouded in mystery since their discovery in 1955. Early investigations suggested that CC arose from either nuclear envelope (NE) or by stacking of endoplasmic reticulum (ER) cisternae. Electron microscopic studies of mitotic HeLa cells suggest that CC are fragments of NE (Fig. 1) that arise as portions of the NE fold back on one another. Because lamin B is a NE specific protein, a monoclonal antibody to lamin B was used to probe the origin of CC. The monoclonal antibody to lamin B was then detected by utilizing a secondary antibody conjugated to the fluorochrome rhodamine which fluoresces red when exposed to UV light. Fluorescent microscopy of interphase HeLa cells demonstrates that only the NE fluoresces red when the cells are probed with a monoclonal antibody to lamin B (Fig. 2). There is no label localized in the cytoplasm which supports the evidence that lamin B is only localized to the NE in interphase cells.

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