scholarly journals Cerebellar Purkinje Cell-Specific Protein-Like Immunoreactivity in Noradrenalin-Chromaffin Cells and Ganglion Cells but Not in Adrenaline-Chromaffin Cells in the Rat Adrenal Medulla

1985 ◽  
Vol 48 (4) ◽  
pp. 421-426 ◽  
Author(s):  
Hisatake KONDO ◽  
Hirofumi KURAMOTO ◽  
Toshihiko IWANAGA ◽  
Tsuneo FUJITA
Keyword(s):  
Purkinje Cell ◽  
Adrenal Medulla ◽  
Chromaffin Cells ◽  
Ganglion Cells ◽  
Specific Protein ◽  
Cerebellar Purkinje Cell

scholarly journals Immunohistochemical evidence for the presence of a vasoactive intestinal peptide, neuropeptide Y and substance P in rat adrenal medulla after heat stress

2012 ◽  
Vol 64 (1) ◽  
pp. 7-13
Author(s):  
Dragana Petrovic-Kosanovic ◽  
Vesna Koko
Keyword(s):  
Heat Stress ◽  
Substance P ◽  
Neuropeptide Y ◽  
Vasoactive Intestinal Peptide ◽  
Adrenal Medulla ◽  
Chromaffin Cells ◽  
Ganglion Cells ◽  
Nerve Fibers ◽  
Acute Heat Stress ◽  
Immunohistochemical Evidence

Immunohistochemistry revealed the presence of VIP-, NPY- and SP-immunoreactivity in the rat adrenal medulla. VIP- and NPY-immunoreactivity was detected in chromaffin and ganglion cells and in nerve fibers, but SP-immunoreactivity was found only in chromaffin cells. After acute heat stress, VIP- and NPY- immunoreactivities in cells and nerve fibers were reduced, probably as a result of the release of these peptides with catecholamines. The absence of SP-immunoreactive ganglion cells in the adrenal medulla suggests that the SP-immunoreactive nerve fibers are extrinsic in origin.

Differential expression of the noradrenaline transporter in adrenergic chromaffin cells, ganglion cells and nerve fibres of the rat adrenal medulla

2001 ◽  
Vol 21 (1) ◽  
pp. 95-104 ◽  
Author(s):  
Jacqueline K. Phillips ◽  
Ratna Dubey ◽  
Erekle Sesiashvilvi ◽  
Mamoru Takeda ◽  
David L. Christie ◽  
...  
Keyword(s):  
Differential Expression ◽  
Adrenal Medulla ◽  
Chromaffin Cells ◽  
Ganglion Cells ◽  
Nerve Fibres ◽  
Noradrenaline Transporter

Morphological Observations on the Granule Types in Rabbit Extra-Adrenal Chromaffin Cells.

1975 ◽  
Vol 33 ◽  
pp. 318-319
Author(s):  
Joe A. Mascorro ◽  
Robert D. Yates
Keyword(s):  
Chromaffin Cells ◽  
Sodium Phosphate ◽  
Ganglion Cells ◽  
Ultrastructural Level ◽  
Osmic Acid ◽  
Adrenal Chromaffin Cells ◽  
Potassium Iodate ◽  
Perfusion Fluid ◽  
New Zealand White Rabbits ◽  
Adrenal Chromaffin

Extra-adrenal chromaffin organs (abdominal paraganglia) constitute rich sources of catecholamines. It is believed that these bodies contain norepinephrine exclusively. However, the present workers recently observed epinephrine type granules in para- ganglion cells. This report investigates catecholamine containing granules in rabbit paraganglia at the ultrastructural level.New Zealand white rabbits (150-170 grams) were anesthetized with 50 mg/kg Nembutal (IP) and perfused with 3% glutaraldehyde buffered with 0.2M sodium phosphate, pH 7.3. The retroperitoneal tissue blocks were removed and placed in perfusion fluid for 4 hours. The abdominal paraganglia were dissected from the blocks, diced, washed in phosphate buffer and fixed in 1% osmic acid buffered with phosphate. In other animals, the glutaraldehyde perfused tissue blocks were immersed for 1 hour in 3% glutaraldehyde/2.5% potassium iodate buffered as before. The paraganglia were then diced, separated into two vials and washed in the buffer. A portion of this tissue received osmic acid fixation.

Immunogold labeling of the calcium binding protein synexin in isolated adrenal chromaffin granules and chromaffin cells

1990 ◽  
Vol 48 (3) ◽  
pp. 952-953
Author(s):  
Gemma A.J. Kuijpers ◽  
Harvey B. Pollard
Keyword(s):  
Adrenal Medulla ◽  
Calcium Binding ◽  
Chromaffin Cells ◽  
Cell Activation ◽  
Structural Information ◽  
Dependent Manner ◽  
Chromaffin Granules ◽  
Immuno Electron Microscopy ◽  
Ultrathin Sections ◽  
Adrenal Chromaffin

Exocytotic fusion of granules in the adrenal medulla chromaffin cell is triggered by a rise in the concentration of cytosolic Ca2+ upon cell activation. The protein synexin, annexin VII, was originally found in the adrenal medulla and has been shown to cause aggregation and to support fusion of chromaffin granules in a Ca2+-dependent manner. We have previously suggested that synexin may there fore play a role in the exocytotic fusion process. In order to obtain more structural information on synexin, we performed immuno-electron microscopy on frozen ultrathin sections of both isolated chromaffin granules and chromaffin cells.Chromaffin granules were isolated from bovine adrenal medulla, and synexin was isolated from bovine lung. Granules were incubated in the presence or absence of synexin (24 μg per mg granule protein) and Ca2+ (1 mM), which induces maximal granule aggregation, in 0.3M sucrose-40m MMES buffer(pH 6.0). Granules were pelleted, washed twice in buffer without synexin and fixed with 2% glutaraldehyde- 2% para formaldehyde in 0.1 M phosphate buffer (GA/PFA) for 30 min. Chromaffin cells were isolated and cultured for 3-5 days, and washed and incubated in Krebs solution with or without 20 uM nicotine. Cells were fixed 90 sec after on set of stimulation with GA/PFA for 30 min. Fixed granule or cell pellets were washed, infiltrated with 2.3 M sucrose in PBS, mounted and frozen in liquid N2.

scholarly journals Erratum: Cerebellar Purkinje cell p75 neurotrophin receptor and autistic behavior

2014 ◽  
Vol 4 (10) ◽  
pp. e476-e476
Author(s):  
L T Lotta ◽  
K Conrad ◽  
D Cory-Slechta ◽  
N F Schor
Keyword(s):  
Purkinje Cell ◽  
Cerebellar Purkinje Cell ◽  
Neurotrophin Receptor ◽  
P75 Neurotrophin Receptor ◽  
Autistic Behavior

The Transcription Factor E2F-1 in SV40 T Antigen-Induced Cerebellar Purkinje Cell Degeneration

1998 ◽  
Vol 12 (1-2) ◽  
pp. 16-28 ◽  
Author(s):  
Maria C. Athanasiou ◽  
Wael Yunis ◽  
Natalie Coleman ◽  
Robert Ehlenfeldt ◽  
H.Brent Clark ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Purkinje Cell ◽  
T Antigen ◽  
Cerebellar Purkinje Cell ◽  
Sv40 T Antigen ◽  
Cell Degeneration ◽  
Purkinje Cell Degeneration ◽  
Transcription Factor E2f
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