scholarly journals Effect of Modification of the NI Artificial Diet on the Biological Fitness Parameters of Mass Reared Western Tarnished Plant Bug,Lygus hesperus

2011 ◽  
Vol 11 (149) ◽  
pp. 1-10 ◽  
Author(s):  
Maribel Portilla ◽  
Gordon Snodgrass ◽  
Doug Streett
Keyword(s):  
Artificial Diet ◽  
Lygus Hesperus ◽  
Tarnished Plant Bug ◽  
Fitness Parameters

scholarly journals Transcriptome-Based Identification of ABC Transporters in the Western Tarnished Plant Bug Lygus hesperus

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e113046 ◽  
Author(s):  
J. Joe Hull ◽  
Kendrick Chaney ◽  
Scott M. Geib ◽  
Jeffrey A. Fabrick ◽  
Colin S. Brent ◽  
...  
Keyword(s):  
Abc Transporters ◽  
Lygus Hesperus ◽  
Tarnished Plant Bug

Adverse Effects of Raw Soybean Extract in Artificial Diet on Survival and Growth of Lygus hesperus Knight

2005 ◽  
Vol 40 (4) ◽  
pp. 390-400 ◽  
Author(s):  
Allen C. Cohen ◽  
Fanrong Zeng ◽  
Patrick Crittenden
Keyword(s):  
Egg Production ◽  
Artificial Diet ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Biomass Accumulation ◽  
Standard Diet ◽  
Total Biomass ◽  
Lygus Hesperus ◽  
Heat Treated ◽  
Soybean Extract

Bioassays were conducted to examine the effects of raw soybean extract in artificial diet on the development, survival, adult weight and biomass accumulation of Lygus hesperus Knight. The diet containing raw soybean extract significantly reduced survival of L. hesperus. Total biomass per rearing unit and survival from eggs to adults were significantly less for L. hesperus fed diet containing raw soybean extract than it was for those fed heat-treated extract, diet with extraction buffer but no extract, or control (standard) diet. Development period, weight of individual adults, and egg production were not significantly different among the four treatments. However, development time was greater for the L. hesperus exposed to raw soy extract, and egg production, as well as individual adult weights, were consistently lower than those fed on the other treatments. Raw and autoclaved soy extracts were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), revealing differences in protein banding patterns, including those from total protein and glycoprotein profiles. Activity of soy trypsin inhibitor (STI) also was measured in raw and heated samples, and it was determined that autoclaving greatly decreased the inhibition activity of this protein. The heat-based deactivation of STI, and the disappearance of most protein bands are most likely associated with the denaturation of proteins and formation of large aggregates that fail to migrate in an electrophoretic field.

scholarly journals Sequencing and De Novo Assembly of the Western Tarnished Plant Bug (Lygus hesperus) Transcriptome

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e55105 ◽  
Author(s):  
J. Joe Hull ◽  
Scott M. Geib ◽  
Jeffrey A. Fabrick ◽  
Colin S. Brent
Keyword(s):  
De Novo Assembly ◽  
De Novo ◽  
Lygus Hesperus ◽  
Tarnished Plant Bug

New Oligidic Production Diet for Lygus hesperus Knight and L. lineolaris (Palisot de Beauvois)2,3

2000 ◽  
Vol 35 (3) ◽  
pp. 301-310 ◽  
Author(s):  
Allen Carson Cohen
Keyword(s):  
Egg Production ◽  
Feeding Habits ◽  
Lima Bean ◽  
Production Costs ◽  
Semisolid Slurry ◽  
Lygus Hesperus ◽  
Tarnished Plant Bug ◽  
Liquid Feeding ◽  
Bean Flour ◽  
The Cost

A new oligidic (undefined) diet for rearing Lygus hesperus Knight (Heteroptera: Miridae) and L. lineolaris (Palisot de Beauvois) is described. The diet (referred to as Nl diet) is a semisolid slurry that accommodates the solid-to-liquid feeding habits of Lygus spp. The Nl diet consists of an “entomophage component” (cooked, whole chicken eggs, chicken egg yolks, sugar, and yeast) combined with plant components (soy bean flour, wheat germ, lima bean meal, and soy lecithin). Biological fitness estimates for L. hesperus indicated that mean biomass production per cage, adult wet and dry weights, survival to the adult stage, and egg production were significantly greater for the Nl diet than for the existing standard, Debolt (1982) diet. The ingredients in the Nl diet cost about ⅛ those in the Debolt diet, and preparation requires less than ½ of the labor. The cost of diet for production per 1000 eggs was approximately $0.004 compared to $0.04 for an equal number of eggs from Debolt diet. Recent work, started after the currently reported bioassays with L. hesperus, indicates that the Nl diet also supports development and reproduction in the tarnished plant bug, L. lineolaris.This diet was used to rear and 15 generations of L. hesperus and is currently being used to support production colonies; it has also been used to rear L. lineolaris for 5 generations, thus far. The L. lineolaris colony started from field collected populations has been reared continuously and exclusively on the Nl diet and is currently in a log phase of population growth. This diet should be beneficial in providing a great reduction of Lygus production costs while producing a high quality, vigorously reproducing insect.

Regulatory roles of biogenic amines and juvenile hormone in the reproductive behavior of the western tarnished plant bug (Lygus hesperus)

2015 ◽  
Vol 186 (2) ◽  
pp. 169-179 ◽  
Author(s):  
Colin S. Brent ◽  
Katelyn Miyasaki ◽  
Connor Vuong ◽  
Brittany Miranda ◽  
Bronwen Steele ◽  
...  
Keyword(s):  
Juvenile Hormone ◽  
Biogenic Amines ◽  
Reproductive Behavior ◽  
Lygus Hesperus ◽  
Tarnished Plant Bug
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