Molecular Cloning and Characterization of G Alpha Proteins from the Western Tarnished Plant Bug, Lygus hesperus

J. Hull; Meixian Wang

doi:10.3390/insects6010054

Characterization of Lygus hesperus (Hemiptera: Miridae) Feeding and Oviposition Injury on Celery Seedlings

Plant Health Progress ◽

10.1094/php-rs-15-0051 ◽

2016 ◽

Vol 17 (2) ◽

pp. 101-105 ◽

Cited By ~ 5

Author(s):

Shimat V. Joseph ◽

Ramon Ahedo ◽

Maria de la Fuente

Keyword(s):

Apium Graveolens ◽

Adult Density ◽

Lygus Hesperus ◽

Central Coast ◽

Tarnished Plant Bug ◽

Crown Area ◽

Diagnostic Symptoms ◽

Coast Region ◽

Dead Tissue

The western tarnished plant bug, Lygus hesperus Knight (Hemiptera: Miridae), can be a serious pest of celery, Apium graveolens dulce (Mill.) Pers. in the Central Coast region of California. Little is known about injury symptoms caused by L. hesperus adults on celery. When celery seedlings were exposed to L. hesperus adults, their crown area was found destroyed with necrotic dead tissue. Destroyed crowns of dead seedlings were observed when seedlings were exposed to five or more L. hesperus adults for ≥24 h but was not observed on seedlings exposed ≤12 h. In addition, elongated lesions were noticed on the celery petiole and were significantly greater when exposed to five or more L. hesperus adults for as little as 4 h. Number of elongated lesion injuries and number of eggs recovered from elongated lesions was correlated (r = 0.66; P < 0.001). Data provide key diagnostic symptoms of L. hesperus feeding and ovipositional injury on celery seedlings. Also, this study expands our understandings on the effects of L. hesperus adult density and duration of exposure which reinforces the need for regular scouting, and immediate management decisions and action to reduce losses from L. hesperus injury in both celery seedlings and mature plants in greenhouse and field settings, respectively Accepted for publication 27 April 2016. Published 4 May 2016.

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Molecular and functional characterization of multiple aquaporin water channel proteins from the western tarnished plant bug, Lygus hesperus

Insect Biochemistry and Molecular Biology ◽

10.1016/j.ibmb.2013.12.002 ◽

2014 ◽

Vol 45 ◽

pp. 125-140 ◽

Cited By ~ 22

Author(s):

Jeffrey A. Fabrick ◽

Jinxin Pei ◽

J. Joe Hull ◽

Andrea J. Yool

Keyword(s):

Functional Characterization ◽

Water Channel ◽

Lygus Hesperus ◽

Tarnished Plant Bug ◽

Channel Proteins ◽

Aquaporin Water Channel

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Molecular cloning and partial characterization of a trypsin-like protein in salivary glands of Lygus hesperus (Hemiptera: Miridae)

Insect Biochemistry and Molecular Biology ◽

10.1016/s0965-1748(01)00123-0 ◽

2002 ◽

Vol 32 (4) ◽

pp. 455-464 ◽

Cited By ~ 22

Author(s):

Fanrong Zeng ◽

Yu-Cheng Zhu ◽

Allen C. Cohen

Keyword(s):

Salivary Glands ◽

Molecular Cloning ◽

Partial Characterization ◽

Lygus Hesperus

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Molecular and Functional Characterization of Pyrokinin-like Peptides in the Western Tarnished Plant Bug Lygus hesperus (Hemiptera: Miridae)

Insects ◽

10.3390/insects12100914 ◽

2021 ◽

Vol 12 (10) ◽

pp. 914

Author(s):

J. Joe Hull ◽

Colin S. Brent ◽

Man-Yeon Choi ◽

Zsanett Mikó ◽

József Fodor ◽

...

Keyword(s):

Insect Cells ◽

Functional Characterization ◽

Biological Functions ◽

Lygus Hesperus ◽

Tarnished Plant Bug ◽

Cerebral Ganglia ◽

Peptide Expression

The pyrokinin (PK) family of insect neuropeptides, characterized by C termini consisting of either WFGPRLamide (i.e., PK1) or FXPRLamide (i.e., PK2), are encoded on the capa and pk genes. Although implicated in diverse biological functions, characterization of PKs in hemipteran pests has been largely limited to genomic, transcriptomic, and/or peptidomic datasets. The Lygus hesperus (western tarnished plant bug) PK transcript encodes a prepropeptide predicted to yield three PK2 FXPRLamide-like peptides with C-terminal sequences characterized by FQPRSamide (LyghePKa), FAPRLamide (LyghePKb), and a non-amidated YSPRF. The transcript is expressed throughout L. hesperus development with greatest abundance in adult heads. PRXamide-like immunoreactivity, which recognizes both pk- and capa-derived peptides, is localized to cells in the cerebral ganglia, gnathal ganglia/suboesophageal ganglion, thoracic ganglia, and abdominal ganglia. Immunoreactivity in the abdominal ganglia is largely consistent with capa-derived peptide expression, whereas the atypical fourth pair of immunoreactive cells may reflect pk-based expression. In vitro activation of a PK receptor heterologously expressed in cultured insect cells was only observed in response to LyghePKb, while no effects were observed with LyghePKa. Similarly, in vivo pheromonotropic effects were only observed following LyghePKb injections. Comparison of PK2 prepropeptides from multiple hemipterans suggests mirid-specific diversification of the pk gene.

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