DNA Strand Breaks Signal the Induction of DNA Double-Strand Break Repair in Saccharomyces cerevisiae

2005 ◽  
Vol 164 (6) ◽  
pp. 781-790 ◽  
Author(s):  
Rakesh Kumar Singh ◽  
Malini Krishna
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Strand Break ◽  
Dna Strand Breaks ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Strand Breaks ◽  
Dna Double Strand Break ◽  
Break Repair ◽  
Dna Strand

scholarly journals Regulation of Error-Prone DNA Double-Strand Break Repair and Its Impact on Genome Evolution

Cells ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 1657 ◽  
Author(s):  
Terrence Hanscom ◽  
Mitch McVey
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Dna Lesions ◽  
Double Strand Break Repair ◽  
End Joining ◽  
Genetic Changes ◽  
Strand Breaks ◽  
Dna Double Strand Break ◽  
Alternative End Joining ◽  
Break Repair

Double-strand breaks are one of the most deleterious DNA lesions. Their repair via error-prone mechanisms can promote mutagenesis, loss of genetic information, and deregulation of the genome. These detrimental outcomes are significant drivers of human diseases, including many cancers. Mutagenic double-strand break repair also facilitates heritable genetic changes that drive organismal adaptation and evolution. In this review, we discuss the mechanisms of various error-prone DNA double-strand break repair processes and the cellular conditions that regulate them, with a focus on alternative end joining. We provide examples that illustrate how mutagenic double-strand break repair drives genome diversity and evolution. Finally, we discuss how error-prone break repair can be crucial to the induction and progression of diseases such as cancer.

scholarly journals Modulation of Saccharomyces cerevisiae DNA double-strand break repair by SRS2 and RAD51.

Genetics ◽  
1995 ◽  
Vol 139 (3) ◽  
pp. 1189-1199
Author(s):  
G T Milne ◽  
T Ho ◽  
D T Weaver
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Recombinational Repair ◽  
Multiple Alleles ◽  
Dna Double Strand Break ◽  
Repair Mechanisms ◽  
Break Repair ◽  
Insight Into

Abstract RAD52 function is required for virtually all DNA double-strand break repair and recombination events in Saccharomyces cerevisiae. To gain greater insight into the mechanism of RAD52-mediated repair, we screened for genes that suppress partially active alleles of RAD52 when mutant or overexpressed. Described here is the isolation of a phenotypic null allele of SRS2 that suppressed multiple alleles of RAD52 (rad52B, rad52D, rad52-1 and KlRAD52) and RAD51 (KlRAD51) but failed to suppress either a rad52 delta or a rad51 delta. These results indicate that SRS2 antagonizes RAD51 and RAD52 function in recombinational repair. The mechanism of suppression of RAD52 alleles by srs2 is distinct from that which has been previously described for RAD51 overexpression, as both conditions were shown to act additively with respect to the rad52B allele. Furthermore, overexpression of either RAD52 or RAD51 enhanced the recombination-dependent sensitivity of an srs2 delta RAD52 strain, suggesting that RAD52 and RAD51 positively influence recombinational repair mechanisms. Thus, RAD52-dependent recombinational repair is controlled both negatively and positively.

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