scholarly journals The RING finger ATPase Rad5p of Saccharomyces cerevisiae contributes to DNA double-strand break repair in a ubiquitin-independent manner

2005 ◽  
Vol 33 (18) ◽  
pp. 5878-5886 ◽  
Author(s):  
S. Chen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ring Finger ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Independent Manner ◽  
Dna Double Strand Break ◽  
Break Repair

scholarly journals Modulation of Saccharomyces cerevisiae DNA double-strand break repair by SRS2 and RAD51.

Genetics ◽  
1995 ◽  
Vol 139 (3) ◽  
pp. 1189-1199
Author(s):  
G T Milne ◽  
T Ho ◽  
D T Weaver
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Recombinational Repair ◽  
Multiple Alleles ◽  
Dna Double Strand Break ◽  
Repair Mechanisms ◽  
Break Repair ◽  
Insight Into

Abstract RAD52 function is required for virtually all DNA double-strand break repair and recombination events in Saccharomyces cerevisiae. To gain greater insight into the mechanism of RAD52-mediated repair, we screened for genes that suppress partially active alleles of RAD52 when mutant or overexpressed. Described here is the isolation of a phenotypic null allele of SRS2 that suppressed multiple alleles of RAD52 (rad52B, rad52D, rad52-1 and KlRAD52) and RAD51 (KlRAD51) but failed to suppress either a rad52 delta or a rad51 delta. These results indicate that SRS2 antagonizes RAD51 and RAD52 function in recombinational repair. The mechanism of suppression of RAD52 alleles by srs2 is distinct from that which has been previously described for RAD51 overexpression, as both conditions were shown to act additively with respect to the rad52B allele. Furthermore, overexpression of either RAD52 or RAD51 enhanced the recombination-dependent sensitivity of an srs2 delta RAD52 strain, suggesting that RAD52 and RAD51 positively influence recombinational repair mechanisms. Thus, RAD52-dependent recombinational repair is controlled both negatively and positively.

scholarly journals The Adenovirus E4orf6 Protein Inhibits DNA Double Strand Break Repair and Radiosensitizes Human Tumor Cells in an E1B-55K-independent Manner

2004 ◽  
Vol 280 (2) ◽  
pp. 1474-1481 ◽  
Author(s):  
Lori S. Hart ◽  
Steven M. Yannone ◽  
Christine Naczki ◽  
Joseph S. Orlando ◽  
Stephen B. Waters ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Human Tumor Cells ◽  
Independent Manner ◽  
Dna Double Strand Break ◽  
Break Repair
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