Cytoskeletal Reorganization and Altered Phagocytotic Ability in Primary Cultures of Rainbow Trout Hemopoietic Tissue Exposed to Low-Level Ionizing Radiation

2005 ◽  
Vol 164 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Peter M. Olwell ◽  
David C. Cottell ◽  
Sharon Ní Shúilleabháin ◽  
Paola Maderna ◽  
Colin Seymour ◽  
...  
2004 ◽  
Vol 162 (2) ◽  
pp. 226-232 ◽  
Author(s):  
F. M. Lyng ◽  
M. Lyons-Alcantara ◽  
P. Olwell ◽  
S. Ní Shuilleabháin ◽  
C. Seymour ◽  
...  

2020 ◽  
Vol 223 (15) ◽  
pp. jeb221069
Author(s):  
Christian Kropf ◽  
Karl Fent ◽  
Stephan Fischer ◽  
Ayako Casanova ◽  
Helmut Segner

ABSTRACTFish gills are a structurally and functionally complex organ at the interface between the organism and the aquatic environment. Gill functions include the transfer of organic molecules, both natural ones and xenobiotic compounds. Whether the branchial exchange of organic molecules involves active transporters is currently not known. Here, we investigated the presence, diversity and functional activity of ATP-binding cassette (ABC) transporters in gills of juvenile rainbow trout. By means of RT-qPCR, gene transcripts of members from the abcb, abcc and abcg subfamilies were identified. Comparisons with mRNA profiles from trout liver and kidney revealed that ABC transporters known to have an apical localization in polarized epithelia, especially abcc2 and abcb1, were under-represented in the gills. In contrast, ABC transporters with mainly basolateral localization showed comparable gene transcript levels in the three organs. The most prominent ABC transporter in gills was an abcb subfamily member, which was annotated as abcb5 based on the synteny and phylogeny. Functional in vivo assays pointed to a role of branchial ABC transporters in branchial solute exchange. We further assessed the utility of primary gill cell cultures to characterize transporter-mediated branchial exchange of organic molecules, by examining ABC transporter gene transcript patterns and functional activity in primary cultures. The gill cultures displayed functional transport activity, but the ABC mRNA expression patterns were different to those of the intact gills. Overall, the findings of this study provide evidence for the presence of functional ABC transporter activity in gills of fish.


1967 ◽  
Vol 24 (12) ◽  
pp. 2539-2548 ◽  
Author(s):  
A. H. Berst ◽  
A. A. Wainio

Of 2614 adult rainbow trout sampled from the Nottawasaga River of Georgian Bay during the period of 1961–67 inclusive, approximately 7.1% had one or more lamprey marks. The trout ranged in fork length from 10 to 33 inches (25 to 84 cm). However, lamprey marks were present only on fish over 16 inches (40 cm) in length. Multiple scarring was found on 30% of the trout that were marked. The incidence of marked fish reached a maximum of 17.2% in the spring of 1962, then declined to 1.3% in the fall of the same year. This decline was coincident with a reduction in the lamprey population after experimental lampricide treatment of the Nottawasaga River and adjacent lamprey spawning streams during 1960 and 1961. The incidence of marked fish remained at a relatively low level for a period of 4 years, then increased substantially, after the presumed recruitment of metamorphosed lampreys during 1966 and 1967.


1990 ◽  
Vol 29 (4) ◽  
pp. 323-327 ◽  
Author(s):  
N. I. Yusifov ◽  
A. M. Kuzin ◽  
F. A. Agaev ◽  
S. G. Alieva

Dose-Response ◽  
2020 ◽  
Vol 18 (4) ◽  
pp. 155932582096959
Author(s):  
Somya Z. Mansour ◽  
Fatma S. M. Moawed ◽  
Monda M. M. Badawy ◽  
Hebatallah E. Mohamed

Bisphenol A (BPA) is a low molecular weight chemical compound that has a deleterious effect on the endocrine system. It was used in plastics manufacturing with injurious effects on different body systems. Occupational exposure to low-level ionizing radiation (<1 Gy) is shown to attenuate an established inflammatory process and therefore enhance cell protection. Therefore, the objective of this study was to investigate the protective effect of boswellic acid (BA) accompanied by whole-body low-dose gamma radiation (γ-R) against BPA-induced lung toxicity in male albino rats. BPA intoxication induced with 500 mg/kg BW. Rats received 50 mg BA/kg BW by gastric gavage concomitant with 0.5 Gy γ-R over 4 weeks. The immunoblotting and biochemical results revealed that BA and/or γ-R inhibited BPA-induced lung toxicity by reducing oxidative damage biomolecules; (MDA and NADPH oxidase gene expression), inflammatory indices (MPO, TNF-α, IL-6, and gene expression of CXCR-4). Moreover, BA and or/γ-R ameliorated the lung inflammation via regulation of the JNK/ERK/c-Fos and Nrf2/ HO-1 signaling pathways. Interestingly, our data demonstrated that BA in synergistic interaction with γ-R is efficacious control against BPA-induced lung injury via anti-oxidant mediated anti-inflammatory activities.


2012 ◽  
Vol 751 (1) ◽  
pp. 7-14 ◽  
Author(s):  
Russel J. Reiter ◽  
Ahmet Korkmaz ◽  
Shuran Ma ◽  
Sergio Rosales-Corral ◽  
Dun-Xian Tan
Keyword(s):  

Biomarkers ◽  
1997 ◽  
Vol 2 (5) ◽  
pp. 287-294 ◽  
Author(s):  
S. Scholz ◽  
I. Behn ◽  
H. Honeck ◽  
C. Hauck ◽  
T. Braunbeck ◽  
...  

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