scholarly journals Ionizing Radiation Induces a Stress Response in Primary Cultures of Rainbow Trout Skin

2004 ◽  
Vol 162 (2) ◽  
pp. 226-232 ◽  
Author(s):  
F. M. Lyng ◽  
M. Lyons-Alcantara ◽  
P. Olwell ◽  
S. Ní Shuilleabháin ◽  
C. Seymour ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Stress Response ◽  
Ionizing Radiation ◽  
Primary Cultures

Cytoskeletal Reorganization and Altered Phagocytotic Ability in Primary Cultures of Rainbow Trout Hemopoietic Tissue Exposed to Low-Level Ionizing Radiation

2005 ◽  
Vol 164 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Peter M. Olwell ◽  
David C. Cottell ◽  
Sharon Ní Shúilleabháin ◽  
Paola Maderna ◽  
Colin Seymour ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Ionizing Radiation ◽  
Primary Cultures ◽  
Cytoskeletal Reorganization ◽  
Low Level ◽  
Hemopoietic Tissue

scholarly journals Effect of ionizing radiation on human skeletal muscle precursor cells

2013 ◽  
Vol 47 (4) ◽  
pp. 376-381 ◽  
Author(s):  
Mihaela Jurdana ◽  
Maja Cemazar ◽  
Katarina Pegan ◽  
Tomaz Mars
Keyword(s):  
Skeletal Muscle ◽  
Stress Response ◽  
Ionizing Radiation ◽  
Human Skeletal Muscle ◽  
Long Term Effects ◽  
Post Irradiation ◽  
Acute Response ◽  
Proliferation Capacity ◽  
Myoblast Proliferation

Abstract Background. Long term effects of different doses of ionizing radiation on human skeletal muscle myoblast proliferation, cytokine signalling and stress response capacity were studied in primary cell cultures. Materials and methods. Human skeletal muscle myoblasts obtained from muscle biopsies were cultured and irradiated with a Darpac 2000 X-ray unit at doses of 4, 6 and 8 Gy. Acute effects of radiation were studied by interleukin - 6 (IL-6) release and stress response detected by the heat shock protein (HSP) level, while long term effects were followed by proliferation capacity and cell death. Results. Compared with non-irradiated control and cells treated with inhibitor of cell proliferation Ara C, myoblast proliferation decreased 72 h post-irradiation, this effect was more pronounced with increasing doses. Post-irradiation myoblast survival determined by measurement of released LDH enzyme activity revealed increased activity after exposure to irradiation. The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6. Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70). Conclusions. Our results show that myoblasts are sensitive to irradiation in terms of their proliferation capacity and capacity to secret IL-6. Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.

scholarly journals ABC transporters in gills of rainbow trout (Oncorhynchus mykiss)

2020 ◽  
Vol 223 (15) ◽  
pp. jeb221069
Author(s):  
Christian Kropf ◽  
Karl Fent ◽  
Stephan Fischer ◽  
Ayako Casanova ◽  
Helmut Segner
Keyword(s):  
Rainbow Trout ◽  
Abc Transporter ◽  
Abc Transporters ◽  
Functional Activity ◽  
Organic Molecules ◽  
Expression Patterns ◽  
Primary Cultures ◽  
Gene Transcript ◽  
Rainbow Trout Oncorhynchus Mykiss

ABSTRACTFish gills are a structurally and functionally complex organ at the interface between the organism and the aquatic environment. Gill functions include the transfer of organic molecules, both natural ones and xenobiotic compounds. Whether the branchial exchange of organic molecules involves active transporters is currently not known. Here, we investigated the presence, diversity and functional activity of ATP-binding cassette (ABC) transporters in gills of juvenile rainbow trout. By means of RT-qPCR, gene transcripts of members from the abcb, abcc and abcg subfamilies were identified. Comparisons with mRNA profiles from trout liver and kidney revealed that ABC transporters known to have an apical localization in polarized epithelia, especially abcc2 and abcb1, were under-represented in the gills. In contrast, ABC transporters with mainly basolateral localization showed comparable gene transcript levels in the three organs. The most prominent ABC transporter in gills was an abcb subfamily member, which was annotated as abcb5 based on the synteny and phylogeny. Functional in vivo assays pointed to a role of branchial ABC transporters in branchial solute exchange. We further assessed the utility of primary gill cell cultures to characterize transporter-mediated branchial exchange of organic molecules, by examining ABC transporter gene transcript patterns and functional activity in primary cultures. The gill cultures displayed functional transport activity, but the ABC mRNA expression patterns were different to those of the intact gills. Overall, the findings of this study provide evidence for the presence of functional ABC transporter activity in gills of fish.

Stress response genes induced in mammalian cells by ionizing radiation

1993 ◽  
Vol 1 (2) ◽  
pp. 81-93 ◽  
Author(s):  
Zvi Fuks ◽  
Adriana Haimovitz-Friedman ◽  
Dennis E. Hallahan ◽  
Donald W. Kufe ◽  
Ralph R. Weichselbaum
Keyword(s):  
Stress Response ◽  
Ionizing Radiation ◽  
Mammalian Cells ◽  
Stress Response Genes

scholarly journals Evidence of major genes affecting stress response in rainbow trout using Bayesian methods of complex segregation analysis1

2009 ◽  
Vol 87 (11) ◽  
pp. 3490-3505 ◽  
Author(s):  
R. L. Vallejo ◽  
C. E. Rexroad ◽  
J. T. Silverstein ◽  
L. L. G. Janss ◽  
G. M. Weber
Keyword(s):  
Rainbow Trout ◽  
Stress Response ◽  
Bayesian Methods ◽  
Major Genes

scholarly journals Development of a monoclonal antibody for ELISA of CYP1A in primary cultures of rainbow trout Oncorhynchus mykiss hepatocytes

Biomarkers ◽  
1997 ◽  
Vol 2 (5) ◽  
pp. 287-294 ◽  
Author(s):  
S. Scholz ◽  
I. Behn ◽  
H. Honeck ◽  
C. Hauck ◽  
T. Braunbeck ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Primary Cultures ◽  
Rainbow Trout Oncorhynchus Mykiss
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