Differential Expression of Oxidative Stress and Extracellular Matrix Remodeling Genes in Low- or High-Dose-Rate Photon-Irradiated Skin

2011 ◽  
Vol 176 (2) ◽  
pp. 187-197 ◽  
Author(s):  
Xiao Wen Mao ◽  
Tsehay Mekonnen ◽  
Ann R. Kennedy ◽  
Daila S. Gridley
Keyword(s):  
Oxidative Stress ◽  
Extracellular Matrix ◽  
Dose Rate ◽  
Differential Expression ◽  
High Dose Rate ◽  
Extracellular Matrix Remodeling ◽  
High Dose ◽  
Matrix Remodeling

scholarly journals Differential expression of immune-associated cancer regulatory genes in low- versus high-dose-rate irradiated AKR/J mice

Genomics ◽  
2011 ◽  
Vol 97 (6) ◽  
pp. 358-363 ◽  
Author(s):  
Suk Chul Shin ◽  
Kyung-Mi Lee ◽  
Yu Mi Kang ◽  
Kwanghee Kim ◽  
Seon Ah Lim ◽  
...  
Keyword(s):  
Dose Rate ◽  
Differential Expression ◽  
High Dose Rate ◽  
Regulatory Genes ◽  
High Dose

Abstract P137: Mir-122 is Associated With Stress-response Protein, Hemeoxygenase-1, for Regulation of Extracellular Matrix Remodeling in Renal Hypertension

Hypertension ◽  
2016 ◽  
Vol 68 (suppl_1) ◽  
Author(s):  
Biswa P Das Purkayastha ◽  
Lu Ren ◽  
Sathnur Pushpakumar ◽  
Utpal Sen
Keyword(s):  
Oxidative Stress ◽  
Extracellular Matrix ◽  
Stress Response ◽  
Mesangial Cells ◽  
Renal Hypertension ◽  
Regulation Of Gene Expression ◽  
Micro Rna ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Ang Ii

Oxidative stress is a major contributing factor in hypertension-induced kidney injury. Hemeoxygenase-1 (Ho-1) is stress response protein constitutively expressed by the proximal tubular epithelial cells in response to oxidative stress. MicroRNAs are single stranded RNA involved in the regulation of gene expression. MicroRNA-122 has been shown to regulate Ho-1 expression in hepatitis; however whether miR-122 regulates Ho-1 in hypertensive kidney is not known. The purpose of the study was to investigate the miRNA-122 Ho-1 regulation and determine its role in extracellular matrix remodeling in renal hypertension. In vitro experiments were done using mesangial cells, treated with/without 200 μM of Angiotensin-II (Ang-II). Ho-1 was induced by ~3.5 folds with Ang-II treatment. miR-122, Ho-1 regulator, was downregulated by >15 times in Ang-II treated cells. In vivo experiments were performed on WT (C57BL6/J) mice aged 12-14 wk and 75-78 wk. The animals were treated with Ang-II (1000ng/kg/min) for 4 weeks. Ho-1 is ~6 folds less in kidney of aged mice as compared to that in the young mice. Hypertension increases miR-122 expression to a greater extent (~5 folds) in aged animals. In Ho-1 knocked down mesangial cells, the extracellular matrix component, Collagen 1A1 (Col1a1), was increased by ~2 folds. In contrast, vascular endothelial growth factor ( Vegf ) and hypoxia-inducible factor ( Hif1 α ) were downregulated in Ho-1 depleted cells. In conclusion, micro RNA, miR-122, transcriptionally regulates Ho-1 as a repressor in kidney and thus affects Ho-1 mediated regulation of the extracellular remodeling in hypertension-induced renal damage.

scholarly journals The Plant Proteinase Inhibitor CrataBL Plays a Role in Controlling Asthma Response in Mice

2018 ◽  
Vol 2018 ◽  
pp. 1-15 ◽  
Author(s):  
Anelize Sartori Santos Bortolozzo ◽  
Adriana Palmeira Dias Rodrigues ◽  
Fernanda Magalhães Arantes-Costa ◽  
Beatriz Mangueira Saraiva-Romanholo ◽  
Flávia Castro Ribas de Souza ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Extracellular Matrix ◽  
Pulmonary Inflammation ◽  
Lung Mechanics ◽  
Elastic Fibers ◽  
Extracellular Matrix Remodeling ◽  
Alveolar Wall ◽  
Polymorphonuclear Cells ◽  
Matrix Remodeling ◽  
And Oxidative Stress

Background. CrataBL is a protein isolated from Crataeva tapia bark. It has been shown to exhibit several biological properties, including anti-inflammatory, analgesic, antitumor, and insecticidal activities. There are no studies evaluating the role of CrataBL in experimental asthma models. Aim. To evaluate the effects of CrataBL on lung mechanics, inflammation, remodeling, and oxidative stress activation of mice with allergic pulmonary inflammation. Materials and Methods. BALB/c mice (6-7 weeks old, 25-30g) were divided into four groups: nonsensitized and nontreated mice (C group, n=8); ovalbumin- (OVA-) sensitized and nontreated mice (OVA group, n=8); nonsensitized and CrataBL-treated mice (C+CR group, n=8); OVA-sensitized and CrataBL-treated mice (OVA+CR group, n=8). We evaluated hyperresponsiveness to methacholine, bronchoalveolar lavage fluid (BALF), pulmonary inflammation, extracellular matrix remodeling, and oxidative stress markers. Results. CrataBL treatment in OVA-sensitized mice (OVA+CR group) attenuated the following variables compared to OVA-sensitized mice without treatment (OVA group) (all p<0.05): (1) respiratory system resistance (Rrs) and elastance (Ers) after methacholine challenge; (2) total cells, macrophages, polymorphonuclear cells, and lymphocytes in BALF; (3) eosinophils and volume fraction of collagen and elastic fibers in the airway and alveolar wall according to histopathological and morphometry analysis; (4) IL-4-, IL-5-, IL-13-, IL-17-, IFN-γ-, MMP-9-, TIMP-1-, TGF-β-, iNOS-, and NF-kB-positive cells and volume of 8-iso-PGF2α in airway and alveolar septa according to immunohistochemistry; and (5) IL-4, IL-5, and IFN-γ according to an ELISA. Conclusion. CrataBL contributes to the control of hyperresponsiveness, pulmonary inflammation, extracellular matrix remodeling, and oxidative stress responses in an animal model of chronic allergic pulmonary inflammation.

Differential expression of cancer pathway-related genes in low-versus high-dose-rate-irradiated AKR/J mice

2012 ◽  
Vol 167 (11) ◽  
pp. 863-870
Author(s):  
Jin Jong Bong ◽  
Yu Mi Kang ◽  
Suk Chul Shin ◽  
Moo Hyun Choi ◽  
Seung Jin Choi ◽  
...  
Keyword(s):  
Dose Rate ◽  
Differential Expression ◽  
High Dose Rate ◽  
High Dose

Differential Expression of Matrix Metalloproteinases and Tissue Inhibitors and Extracellular Matrix Remodeling in Aortic Regurgitant Hearts

Cardiology ◽  
2009 ◽  
Vol 113 (3) ◽  
pp. 161-168 ◽  
Author(s):  
Sharada L. Truter ◽  
Daniel F. Catanzaro ◽  
Phyllis G. Supino ◽  
Anuj Gupta ◽  
John Carter ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Matrix Metalloproteinases ◽  
Differential Expression ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Tissue Inhibitors
Sign in / Sign up

Export Citation Format

Share Document