In VitroandIn VivoStudies of Boron Neutron Capture Therapy: Boron Uptake/Washout and Cell Death

2011 ◽  
Vol 175 (4) ◽  
pp. 452-462 ◽  
Author(s):  
C Ferrari ◽  
J Bakeine ◽  
F Ballarini ◽  
A Boninella ◽  
S Bortolussi ◽  
...  
Keyword(s):  
Cell Death ◽  
Boron Neutron Capture Therapy ◽  
Neutron Capture ◽  
Neutron Capture Therapy ◽  
Boron Uptake ◽  
Boron Neutron Capture

Boron uptake measurements in a rat model for Boron Neutron Capture Therapy of lung tumours

2011 ◽  
Vol 69 (2) ◽  
pp. 394-398 ◽  
Author(s):  
S. Bortolussi ◽  
J.G. Bakeine ◽  
F. Ballarini ◽  
P. Bruschi ◽  
M.A. Gadan ◽  
...  
Keyword(s):  
Rat Model ◽  
Boron Neutron Capture Therapy ◽  
Neutron Capture ◽  
Neutron Capture Therapy ◽  
Boron Uptake ◽  
Lung Tumours ◽  
Boron Neutron Capture

scholarly journals YC-1 sensitizes the antitumor effects of boron neutron capture therapy in hypoxic tumor cells

2020 ◽  
Vol 61 (4) ◽  
pp. 524-534
Author(s):  
Takaomi Harada ◽  
Katsumi Hirose ◽  
Yuki Wada ◽  
Mariko Sato ◽  
Koji Ichise ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Boron Neutron Capture Therapy ◽  
Neutron Capture ◽  
Neutron Capture Therapy ◽  
Boron Uptake ◽  
Antitumor Effects ◽  
Hypoxic Conditions ◽  
Boron Neutron Capture ◽  
Hypoxic Tumor ◽  
In Cells

Abstract The uptake of boron into tumor cells is a key factor in the biological effects of boron neutron capture therapy (BNCT). The uptake of boron agents is suppressed in hypoxic conditions, but the mechanism of hypoxia-induced modulation of suppression of boron uptake is not clear. Therefore, we evaluated whether hypoxia-inducible factor 1α (HIF-1α) contributes to attenuation of the antitumor effects of BNCT in hypoxic tumor cells. We also tested whether YC-1, a HIF-1α-targeting inhibitor, has therapeutic potential with BNCT. To elucidate the mechanism of attenuation of the effects of BNCT caused by hypoxia, deferoxamine (DFO) was used in experiments. Cells were incubated in normal oxygen, hypoxic conditions (1% O2) or 5 μM DFO for 24 h. Then, cells were treated with 10B-boronophenylalanine (BPA) for 2 h and boron accumulation in cells was evaluated. To clarify the relationship between HIF-1α and L-type amino acid transporter 1 (LAT1), gene expression was evaluated by a using HIF-1α gene knockdown technique. Finally, to improve attenuation of the effects of BNCT in hypoxic cells, BNCT was combined with YC-1. Boron uptake was continuously suppressed up to 2 h after administration of BPA by 5 μM DFO treatment. In cells treated with 5 μM DFO, LAT1 expression was restored in HIF-1α-knocked down samples in all cell lines, revealing that HIF-1α suppresses LAT1 expression in hypoxic cells. From the results of the surviving fraction after BNCT combined with YC-1, treatment with YC-1 sensitized the antitumor effects of BNCT in cells cultured in hypoxia.

scholarly journals Analysis of Radiation Interactions and Biological Effects for Boron Neutron Capture Therapy

2018 ◽  
Vol 35 (3) ◽  
pp. 203-207
Author(s):  
Ren-Tai Chiang
Keyword(s):  
Cell Death ◽  
Cancer Cells ◽  
Blood Supply ◽  
Boron Neutron Capture Therapy ◽  
Neutron Capture ◽  
Biological Effects ◽  
Neutron Capture Therapy ◽  
Boron Neutron Capture ◽  
Cancer Tumor ◽  
Ionizing Radiations

 The direct and indirect ionizing radiation sources for boron neutron capture therapy (BNCT)are identi?ed. The mechanisms of physical, chemical and biological radiation interactions for BNCT are systematically described and analyzed. The relationship between the effect of biological radiation and radiation dose are illustrated and analyzed for BNCT. If the DNAs in chromosomes are damaged by ion- izing radiations, the instructions that control the cell function and reproduction are also damaged. This radiation damage may be reparable, irreparable, or incorrectly repaired. The irreparable damage can result in cell death at next mitosis while incorrectly repaired damage can result in mutation. Cell death leads to variable degrees of tissue dysfunction, which can affect the whole organism’s functions. Can- cer cells cannot live without oxygen and nutrients via the blood supply. A cancer tumor can be shrunk by damaging angiogenic factors and/or capillaries via ionizing radiations to decrease blood supply into the cancer tumor. The collisions between ionizing radiations and the target nuclei and the absorption of the ultraviolet, visible light, infrared and microwaves from bremsstrahlung in the tumor can heat up and damage cancer cells and function as thermotherapy. The cancer cells are more chemically and biologically sensitive at the BNCT-induced higher temperatures since free-radical-induced chemical re- actions are more random and vigorous at higher temperatures after irradiation, and consequently the cancer cells are harder to divide or even survive due to more cell DNA damage. BNCT is demonstrated via a recent clinical trial that it is quite effective in treating recurrent nasopharyngeal cancer.

Toward clinical application of prompt gamma spectroscopy forin vivomonitoring of boron uptake in boron neutron capture therapy

2001 ◽  
Vol 28 (5) ◽  
pp. 787-795 ◽  
Author(s):  
P. M. Munck af Rosenschöld ◽  
W. F. A. R. Verbakel ◽  
C. P. Ceberg ◽  
F. Stecher-Rasmussen ◽  
B. R. R. Persson
Keyword(s):  
Clinical Application ◽  
Boron Neutron Capture Therapy ◽  
Neutron Capture ◽  
Gamma Spectroscopy ◽  
Prompt Gamma ◽  
Neutron Capture Therapy ◽  
Boron Uptake ◽  
Boron Neutron Capture

Boron uptake in normal melanocytes and melanoma cells and boron biodistribution study in mice bearing B16F10 melanoma for boron neutron capture therapy

2012 ◽  
Vol 51 (3) ◽  
pp. 319-329 ◽  
Author(s):  
Fernanda Faião-Flores ◽  
Paulo Rogério Pinto Coelho ◽  
João Dias Toledo Arruda-Neto ◽  
Maria Aparecida Pires Camillo ◽  
Silvya Stuchi Maria-Engler ◽  
...  
Keyword(s):  
Boron Neutron Capture Therapy ◽  
Neutron Capture ◽  
Melanoma Cells ◽  
Biodistribution Study ◽  
Neutron Capture Therapy ◽  
Boron Uptake ◽  
B16f10 Melanoma ◽  
Boron Neutron Capture
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