Presumptive Identification of a Novel Adenovirus in a Harris Hawk (Parabuteo unicinctus), a Bengal Eagle Owl (Bubo bengalensis), and a Verreaux's Eagle Owl (Bubo lacteus)

2006 ◽  
Vol 20 (2) ◽  
pp. 105-112 ◽  
Author(s):  
Petra Zsivanovits ◽  
Deborah J. Monks ◽  
Neil A. Forbes ◽  
Krisztina Ursu ◽  
Rüdiger Raue ◽  
...  
2011 ◽  
Author(s):  
Desiree I. Sharpe ◽  
Erin N. Colbert-White ◽  
E. Monteen McCord

2011 ◽  
Author(s):  
James F. Dwyer ◽  
James C. Bednarz
Keyword(s):  

2020 ◽  
Author(s):  
Denver W. Holt ◽  
Regan Berkley ◽  
Caroline Deppe ◽  
Paula L. Enríquez ◽  
Julie L. Petersen ◽  
...  
Keyword(s):  

2020 ◽  
Author(s):  
Denver W. Holt ◽  
Regan Berkley ◽  
Caroline Deppe ◽  
Paula L. Enríquez ◽  
Julie L. Petersen ◽  
...  
Keyword(s):  

Author(s):  
Jorge Tobajas ◽  
Carlos Rouco ◽  
Javier Fernandez-de-Simon ◽  
Francisco Díaz-Ruiz ◽  
Francisca Castro ◽  
...  

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 252
Author(s):  
Valerie E. Ryman ◽  
Felicia M. Kautz ◽  
Steve C. Nickerson

Staphylococcus aureus is one of the most concerning mastitis-causing pathogens in dairy cattle. Using basic microbiological techniques, S. aureus is typically identified by colony characteristics and hemolysis on blood agar where isolates without hemolysis are typically considered to be coagulase-negative staphylococci (CNS) isolates. Herein, we present a decade-long case study where suspected S. aureus isolates from one Georgia dairy farm were further tested to confirm presumptive identification. Presumptive identification of bacterial growth from 222 mammary secretions from bred Holstein heifers and lactating cows was conducted at the time of collection. Presumptive identification of S. aureus on blood agar was based on observation of colony morphology, color, and presence or absence of a broad zone of incomplete hemolysis and a smaller zone of complete hemolysis at 48 h. Those without hemolysis were presumptively characterized as CNS. All isolates were further plated on mannitol salt agar and a coagulase test was performed. A positive for both of these tests together was deemed to be S. aureus. A selection of isolates was tested using API® Staph to biochemically confirm S. aureus identification. Data showed that 63.96% of isolates presumed to be CNS isolates were identified as S. aureus, 9.46% of isolates presumed to be CNS isolates were identified as coagulase-positive staphylococci (CPS) species (but not S. aureus), and 26.58% of samples that were presumed to be CNS isolates were identified correctly.


1986 ◽  
Vol 49 (6) ◽  
pp. 471-476 ◽  
Author(s):  
PETER J. SLADE ◽  
MOHAMMED A. FALAH ◽  
AHMED M. R. AI-GHADY

A total of 139 replicate samples of water were tested for Aeromonas hydrophila and coliforms. These consisted of 95 replicates from bottled mineral water, 13 replicates from flower petal infusions and 31 samples of domestic municipality supplies. Of these, 59 (43%) were positive for A. hydrophila, 15 (11%) were positive for coliforms and 11 (8%) positive for both A. hydrophila and coliforms. Most of the isolates of A. hydrophila came from various batches of one brand of bottled mineral water, none of which contained coliforms. The organism was isolated more frequently from newer samples, particularly those bottled for 59 d or less. Samples of treated water from one municipality were free from coliforms and A. hydrophila. Chlorinated water from another town was free from coliforms, but some samples contained A. hydrophila. In unchlorinated water from a third municipal source, there was a high degree of correlation between incidence of A. hydrophila and presence of coliforms. A selective method, using media without antibiotics, for isolation of A. hydrophila was used. A novel medium for the presumptive identification of A. hydrophila, gelatin arginine dihydrolase (GAD) medium, was assessed, with confirmation of suspected isolates using the API 20E system. Of 109 isolates from two selective agars identified with the organism on API strips, 18 (16.5%) were falsely gelatinase negative in GAD medium, of which 9 (8.3%) also gave false-negative arginine dihydrolase reactions. Of those presumptively identified as A. hydrophila in GAD, 4/95 isolates (4.2% false-positives) were not confirmed.


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