scholarly journals CHARACTERIZATION OF PATHOLOGY AND PARASITE LOAD IN OUTBRED AND INBRED MOUSE MODELS OF CHRONIC NEOSPORA CANINUM INFECTION

2004 ◽  
Vol 90 (3) ◽  
pp. 579-583 ◽  
Author(s):  
Esther Collantes-Fernández ◽  
Gema Álvarez-García ◽  
Valentín Pérez-Pérez ◽  
Juana Pereira-Bueno ◽  
Luis Miguel Ortega-Mora
Keyword(s):  
Mouse Models ◽  
Neospora Caninum ◽  
Inbred Mouse ◽  
Parasite Load ◽  
Caninum Infection

Characterization of an Outbred Pregnant Mouse Model of Neospora caninum Infection

2002 ◽  
Vol 88 (4) ◽  
pp. 691
Author(s):  
Helen E. Quinn ◽  
Catherine M. D. Miller ◽  
Cheryl Ryce ◽  
Peter A. Windsor ◽  
John T. Ellis
Keyword(s):  
Mouse Model ◽  
Neospora Caninum ◽  
Pregnant Mouse ◽  
Caninum Infection

CHARACTERIZATION OF AN OUTBRED PREGNANT MOUSE MODEL OF NEOSPORA CANINUM INFECTION

2002 ◽  
Vol 88 (4) ◽  
pp. 691-696 ◽  
Author(s):  
Helen E. Quinn ◽  
Catherine M. D. Miller ◽  
Cheryl Ryce ◽  
Peter A. Windsor ◽  
John T. Ellis
Keyword(s):  
Mouse Model ◽  
Neospora Caninum ◽  
Pregnant Mouse ◽  
Caninum Infection

RecNcMIC3-1-R is a microneme- and rhoptry-based chimeric antigen that protects against acute neosporosis and limits cerebral parasite load in the mouse model for Neospora caninum infection

Vaccine ◽  
2011 ◽  
Vol 29 (40) ◽  
pp. 6967-6975 ◽  
Author(s):  
Thierry Monney ◽  
David Rütti ◽  
Michelle Schorer ◽  
Karim Debache ◽  
Denis Grandgirard ◽  
...  
Keyword(s):  
Mouse Model ◽  
Neospora Caninum ◽  
Parasite Load ◽  
Caninum Infection

scholarly journals Temporal Distribution and Parasite Load Kinetics in Blood and Tissues during Neospora caninum Infection in Mice

2006 ◽  
Vol 74 (4) ◽  
pp. 2491-2494 ◽  
Author(s):  
Esther Collantes-Fernández ◽  
Inmaculada López-Pérez ◽  
Gema Álvarez-García ◽  
Luis M. Ortega-Mora
Keyword(s):  
Acute Phase ◽  
Neospora Caninum ◽  
Clinical Signs ◽  
Chronic Phase ◽  
Temporal Distribution ◽  
Parasite Load ◽  
Caninum Infection ◽  
Kinetics Of ◽  
The Brain

ABSTRACT The kinetics of Neospora caninum loads in mice inoculated with NC-Liv or NC-1 isolates were studied. The acute phase was characterized by parasitemia and the detection of parasite DNA in several organs, whereas during the chronic phase, the parasite was detected mainly in the brain. Mice infected with NC-Liv developed clinical signs, showing higher brain parasite burdens than NC-1-infected mice.

scholarly journals Microneme Protein 6 Is Involved in Invasion and Egress by Neospora caninum

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 201
Author(s):  
Xianmei Wang ◽  
Di Tang ◽  
Fei Wang ◽  
Gaowei Jin ◽  
Lifang Wang ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Parasite Load ◽  
Host Cells ◽  
Host Cell Invasion ◽  
Caninum Infection ◽  
Apicomplexan Parasites ◽  
Key Steps ◽  
Microneme Protein ◽  
Adhesion And Invasion ◽  
Microneme Proteins

Background: Neospora caninum, is the etiological agent of neosporosis, an infection that causes abortions in cattle and nervous system dysfunction in dogs. Invasion and egress are the key steps of the pathogenesis of N. caninum infection. Microneme proteins (MICs) play important roles in the recognition, adhesion, and invasion of host cells in other apicomplexan parasites. However, some MICs and their functions in N. caninum infection have rarely been reported. Methods: The homologous recombination strategy was used to investigate the function of MIC6 in N. caninum infection. Results: ΔNcMIC6 showed a smaller plaque size and weakened capacities of invasion and egress than Nc1. Transcription levels of the egress-related genes CDPK1, PLP1, and AMA1 of ΔNcMIC6 were downregulated. Due to the lack of NcMIC6, virulence of the pathogen in the infected mouse was weakened. The subcellular localization of NcMIC1 and NcMIC4 in ΔNcMIC6, however, did not change. Nevertheless, the transcription levels of MIC1 and MIC4 in ΔNcMIC6 were downregulated, and the expression and secretion of MIC1 and MIC4 in ΔNcMIC6 were reduced compared with that in Nc1. Furthermore, the absence of NcMIC6 weakened the virulence in mice and lower parasite load detected in mice brains. Conclusions: NcMIC6 is involved in host cell invasion and egress in N. caninum and may work synergistically with other MICs to regulate the virulence of the pathogen. These data lay a foundation for further research into the function and application of NcMIC6.

Pathological and immunological findings in placentas from pregnant BALB/c mice infected with Neospora caninum at early and late stages of gestation

2011 ◽  
Vol 56 (3) ◽  
Author(s):  
Inmaculada López-Pérez ◽  
Esther Collantes-Fernández ◽  
Silvia Rojo-Montejo ◽  
Vanesa Navarro-Lozano ◽  
Verónica Risco-Castillo ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Placental Tissue ◽  
Parasite Load ◽  
Late Gestation ◽  
Histopathological Analysis ◽  
Caninum Infection ◽  
Tnf Α ◽  
Ifn Γ ◽  
Late Stages

AbstractNeospora caninum is transmitted from a cow to its foetus by vertical transmission and the timing of infection in gestation is an important factor in determining the disease outcome. Few studies have explored the role of the placenta in the outcome of N. caninum infection during pregnancy. Here, we described the N. caninum presence, parasite load, local immune response, and histopathological lesions at the materno-foetal interface after infection of BALB/c mice at early and late stages of gestation. In mice infected at early gestation, N. caninum DNA was detected in foetoplacentary units 7 days post-infection (PI) and in the placenta, but not in viable foetuses on day 14 PI, indicating that the parasite was multiplying primarily in the placental tissues without reaching the foetus. Moreover, parasite DNA was detected in resorptions, suggesting that foetal death could be a consequence of infection. An increase in IFN-γ, TNF-α and IL-10 expression was observed in N. caninum PCR-positive placentas, which could favour N. caninum foetal transmission and be harmful to both the placenta and the foetus. Histopathological analysis revealed necrosis affecting both the maternal and foetal sides of the placenta. At late gestation, transmission occurred rapidly following infection (day 3 PI), but parasite were rarely found. In addition, an increase in cytokine expression was observed in spleen and placental tissues from infected animals, while a downregulation in IL-4 expression was only observed in the spleen. Finally, necrosis in the placenta was limited to the maternal side, suggesting that the parasite is mainly multiplying in the placental tissue at this stage. Thus, the results of the present study indicate that the placenta may be actively involved in N. caninum pathogenesis.

scholarly journals Histopathological findings and immunohistochemical staining of Neospora caninum infection in placentas of animals for slaughter

2018 ◽  
Vol 39 (5) ◽  
pp. 2287
Author(s):  
Allana Freitas Barros ◽  
Higor Ferreira Silva ◽  
Renata Mondêgo de Oliveira ◽  
Nathálya Dos Santos Martins ◽  
Mylena Andréa Oliveira Torres ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Neospora Caninum ◽  
Small Ruminants ◽  
Parasitic Disease ◽  
Histopathological Changes ◽  
Cattle Production ◽  
Caninum Infection ◽  
Bovine Neosporosis ◽  
The City

Bovine neosporosis is a parasitic disease caused by the protozoan Neospora caninum, which is one of the main pathogens that cause abortions in cattle worldwide. It is responsible for large losses in dairy and beef cattle, especially with regard to the expenses associated with decreased productivity and increased discard. In the State of Maranhão, the occurrence of this parasite has already been confirmed in the dairy cattle of three microregions that make up the state’s dairy industry and in small ruminants of the municipalities of Amarante do Maranhão and Buritirana. This fact justifies the importance of determining the presence of N. caninum in cattle slaughtered in the slaughterhouses of the Island of São Luís, to help control the parasitic cycle in the cattle production system of the region under study, and the importance of its diagnosis. The objective of the research was to conduct a histopathological and immunohistochemical characterization of bovine placentas infected with N. caninum collected in slaughterhouses. Sixteen placenta samples from cows were randomly selected at the slaughterhouses of the city of São Luís for histological analysis and immunohistochemistry. Results showed that 31% of the placentas presented histopathological changes such as: inflammatory process, edema, hemorrhage and presence of hemosiderin. All slides with histopathological changes showed immunolabeling for N. caninum.

scholarly journals Characterization of tumor‐associated macrophages in prostate cancer transgenic mouse models

The Prostate ◽  
2021 ◽  
Author(s):  
Amber E. Groot ◽  
Kayla V. Myers ◽  
Timothy E. G. Krueger ◽  
Ashley L. Kiemen ◽  
Natalia H. Nagy ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Transgenic Mouse ◽  
Mouse Models ◽  
Transgenic Mouse Models ◽  
Tumor Associated Macrophages

scholarly journals Neospora caninum Infection in a Litter of Labrador Retriever Dogs in Denmark

1995 ◽  
Vol 36 (3) ◽  
pp. 387-391
Author(s):  
A. Flagstad ◽  
Η. Ε. Jensen ◽  
Ι. Bjerkàs ◽  
Κ. Rasmussen
Keyword(s):  
Neospora Caninum ◽  
Labrador Retriever ◽  
Caninum Infection

Seroprevalence of and risk factors for Neospora caninum infection in yaks ( Bos grunniens ) in China

2017 ◽  
Vol 242 ◽  
pp. 22-23 ◽  
Author(s):  
Qing-Feng Meng ◽  
Gui-Zhe Yao ◽  
Si-Yuan Qin ◽  
Jian Wu ◽  
Xi-Chen Zhang ◽  
...  
Keyword(s):  
Risk Factors ◽  
Neospora Caninum ◽  
Caninum Infection ◽  
Bos Grunniens
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