COMPARISON OF AGAROSE GEL AND CAPILLARY ZONE ELECTROPHORESIS METHODS USING PLASMA FROM GREEN TURTLES (CHELONIA MYDAS)

2020 ◽  
Vol 51 (1) ◽  
pp. 123
Author(s):  
Madison Toonder ◽  
Justin R. Perrault ◽  
Carolyn Cray
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Chelonia Mydas ◽  
Agarose Gel ◽  
Green Turtles ◽  
Zone Electrophoresis ◽  
Capillary Zone

Measurement of serum monoclonal components: comparison between densitometry and capillary zone electrophoresis

2006 ◽  
Vol 44 (5) ◽  
Author(s):  
Michele Mussap ◽  
Francesco Pietrogrande ◽  
Silvia Ponchia ◽  
Piero Maria Stefani ◽  
Roberto Sartori ◽  
...  
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Quantitative Measurement ◽  
Protein Quantification ◽  
M Protein ◽  
Agarose Gel ◽  
Altman Analysis ◽  
Monoclonal Gammopathies ◽  
M Proteins ◽  
Zone Electrophoresis ◽  
Capillary Zone

AbstractQuantitative measurement of serum monoclonal protein (M-protein) is one of the most important tools for monitoring disease activity in monoclonal gammopathies. The aims of this study were to evaluate serum M-protein quantification by capillary zone electrophoresis (CZE) and to compare results with those obtained by densitometric scanning of high-resolution agarose gel electrophoresis (HRE-AGE). The evaluation was carried out on 82 samples from patients with various monoclonal gammopathies. All the suspected M-proteins were confirmed and characterised by immunofixation on agarose gel (IFE). CZE was performed on a Paragon CZE™ 2000 system (Beckman Coulter). Passing-Bablok regression was: y (CZE)=1.27×(HRE-AGE)–5.21g/L. The correlation coefficient was 0.92. Bland-Altman analysis demonstrated a mean difference of −1.83g/L (95% CI −0.76 to −2.90) with clear evidence of a concentration-related bias. Densitometry gave higher values at low M-spikes (<20g/L), whereas CZE gave higher values at large M-spikes (>20g/L). The concentration-related bias was found to be independent of the immunoglobulin isotype. In conclusion, to compare previous results obtained by M-protein densitometric scanning with those obtained by direct measurement of CZE peaks, the calculation of a univocal transforming factor appears to be unreliable.

A comparison of resolution of DNA fragments between agarose gel and capillary zone electrophoresis in agarose solutions

1995 ◽  
Vol 16 (1) ◽  
pp. 1345-1353 ◽  
Author(s):  
George H. Weiss ◽  
Mark Garner ◽  
Elena Yarmola ◽  
Petr Boček ◽  
Andreas Chrambach
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Agarose Gel ◽  
Dna Fragments ◽  
Zone Electrophoresis ◽  
Capillary Zone
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