Measurement of serum monoclonal components: comparison between densitometry and capillary zone electrophoresis

2006 ◽  
Vol 44 (5) ◽  
Author(s):  
Michele Mussap ◽  
Francesco Pietrogrande ◽  
Silvia Ponchia ◽  
Piero Maria Stefani ◽  
Roberto Sartori ◽  
...  
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Quantitative Measurement ◽  
Protein Quantification ◽  
M Protein ◽  
Agarose Gel ◽  
Altman Analysis ◽  
Monoclonal Gammopathies ◽  
M Proteins ◽  
Zone Electrophoresis ◽  
Capillary Zone

AbstractQuantitative measurement of serum monoclonal protein (M-protein) is one of the most important tools for monitoring disease activity in monoclonal gammopathies. The aims of this study were to evaluate serum M-protein quantification by capillary zone electrophoresis (CZE) and to compare results with those obtained by densitometric scanning of high-resolution agarose gel electrophoresis (HRE-AGE). The evaluation was carried out on 82 samples from patients with various monoclonal gammopathies. All the suspected M-proteins were confirmed and characterised by immunofixation on agarose gel (IFE). CZE was performed on a Paragon CZE™ 2000 system (Beckman Coulter). Passing-Bablok regression was: y (CZE)=1.27×(HRE-AGE)–5.21g/L. The correlation coefficient was 0.92. Bland-Altman analysis demonstrated a mean difference of −1.83g/L (95% CI −0.76 to −2.90) with clear evidence of a concentration-related bias. Densitometry gave higher values at low M-spikes (<20g/L), whereas CZE gave higher values at large M-spikes (>20g/L). The concentration-related bias was found to be independent of the immunoglobulin isotype. In conclusion, to compare previous results obtained by M-protein densitometric scanning with those obtained by direct measurement of CZE peaks, the calculation of a univocal transforming factor appears to be unreliable.

scholarly journals Detection and identification of low-concentration M proteins with CZE/IS; a 'dry' analysis

2005 ◽  
Vol 42 (2) ◽  
pp. 133-135 ◽  
Author(s):  
Irene MLW Keularts ◽  
Marlene H Beunis ◽  
John van Oord ◽  
Johannes W Janssen
Keyword(s):  
Capillary Zone Electrophoresis ◽  
M Protein ◽  
Light Chains ◽  
Detection Accuracy ◽  
Low Concentration ◽  
Detection And Identification ◽  
M Proteins ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Protein Spectra

The accuracy of detection and identification of low-concentration M proteins using capillary zone electrophoresis/immunosubtraction (CZE/IS) plots was systematically studied. To this end, 49 CZE plots (protein spectra; session I) and CZE/IS plots (spectra with corresponding immunosubtraction plots; session II) were judged by participants from 12 Dutch laboratories. Plots were based on mixed sera with a low concentration (0.4, 1 or 4 g/L) of one M protein, as well as normal sera, sera with an oligoclonal or polyclonal increase in immunoglobulins, and sera containing free monoclonal light chains. At 0.4 g/L, nine out of 12 (9/12) different M proteins were hard to detect. At 1 g/L, 6/12 M proteins were detected by >85% of the participants. At 4 g/L, 10/12 M proteins were detected by all participants. Normal spectra were always judged correctly. At 1 g/L and 4 g/L, detected M proteins were identified correctly by 92% of the participants, versus only 63% at 0.4 g/L. The detection accuracy was only slightly (non-significantly) improved by the application of IS plots. Our data indicate that CZE/IS is applicable for most M proteins at concentrations ≥4 g/L.

scholarly journals Inability to Measure M-Protein With Capillary Zone Electrophoresis (CAPPILLARYS 2) in Tracings With NonDiscernable Peaks

2014 ◽  
Vol 29 (5) ◽  
pp. 343-346
Author(s):  
Richard S. P. Huang ◽  
Amitava Dasgupta ◽  
Andy N. D. Nguyen ◽  
Amer Wahed
Keyword(s):  
Capillary Zone Electrophoresis ◽  
M Protein ◽  
Zone Electrophoresis ◽  
Capillary Zone

A comparison of resolution of DNA fragments between agarose gel and capillary zone electrophoresis in agarose solutions

1995 ◽  
Vol 16 (1) ◽  
pp. 1345-1353 ◽  
Author(s):  
George H. Weiss ◽  
Mark Garner ◽  
Elena Yarmola ◽  
Petr Boček ◽  
Andreas Chrambach
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Agarose Gel ◽  
Dna Fragments ◽  
Zone Electrophoresis ◽  
Capillary Zone
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