Factors Influencing the Attenuation of Serotype 1 Marek'S Disease Virus by Serial Cell Culture Passage and Evaluation of Attenuated Strains for Protection and Replication

2009 ◽  
Vol 4 (1) ◽  
pp. e12-e13
Author(s):  
Ekaterina Dudnikova ◽  
Anatoly Vlasov ◽  
Svetlana Norkina ◽  
Dmitry Kireev ◽  
Richard L. Witter
Keyword(s):  
Cell Culture ◽  
Disease Virus ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Factors Influencing ◽  
Culture Passage ◽  
Serotype 1 ◽  
Cell Culture Passage

Genome Analysis of Marek's Disease Virus Strain CVI-988: Effect of Cell Culture Passage on the Inverted Repeat Regions

1999 ◽  
Vol 43 (2) ◽  
pp. 182 ◽  
Author(s):  
B. J. L. Hooft van Iddekinge ◽  
L. Stenzler ◽  
K. A. Schat ◽  
H. Boerrigter ◽  
G. Koch
Keyword(s):  
Cell Culture ◽  
Disease Virus ◽  
Virus Strain ◽  
Genome Analysis ◽  
Inverted Repeat ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Culture Passage ◽  
Cell Culture Passage

Expression of Marek's disease virus (MDV) serotype 2 gene which has partial homology with MDV serotype 1 pp38 gene

1995 ◽  
Vol 35 (2) ◽  
pp. 223-229 ◽  
Author(s):  
Mitsuru Ono ◽  
Ken Maeda ◽  
Yasushi Kawaguchi ◽  
Hyung-Kwan Jang ◽  
Yukinobu Tohya ◽  
...  
Keyword(s):  
Disease Virus ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Serotype 1 ◽  
Pp38 Gene ◽  
Partial Homology

scholarly journals Quantitative profiling of the shedding rate of the three Marek's disease virus (MDV) serotypes reveals that challenge with virulent MDV markedly increases shedding of vaccinal viruses

2007 ◽  
Vol 88 (8) ◽  
pp. 2121-2128 ◽  
Author(s):  
Aminul Islam ◽  
Stephen W. Walkden-Brown
Keyword(s):  
Disease Virus ◽  
Broiler Chickens ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Commercial Broiler ◽  
Virulent Virus

The shedding profile of Marek's disease virus serotype 1 (MDV1, virulent), serotype 2 (MDV2, vaccinal) and herpesvirus of turkeys (HVT, vaccinal) in commercial broiler chickens was determined by measuring the daily rate of production of feather dander from chickens housed in isolators and by quantifying the viral load of each of these serotypes in the dander using quantitative real-time PCR (qPCR). MDV1 and HVT viruses were detectable in dander filtered from isolator exhaust air from day 7 and MDV2 from day 12 after infection and thereafter until the end of the experiment at 61 days of age of the chickens. There was no difference in shedding rate among the three MDV1 isolates. Daily shedding of MDV1 increased sharply between days 7 and 28 and stabilized thereafter at about 109 virus copies per chicken per day, irrespective of vaccination status. Challenge with the three different MDV1 isolates markedly increased shedding of the vaccinal viruses HVT and MDV2 in dander by 38- and 75-fold, respectively. These results demonstrate the utility of qPCR for the differentiation and quantification of different MDV serotypes in feather dander and have significant implications for the routine monitoring of Marek's disease using qPCR assays of dust, for epidemiological modelling of the behaviour and spread of MDVs in chicken populations and for studies into the evolution of virulence in MDV1 in the face of blanket vaccination with imperfect vaccines that ameliorate disease but do not prevent infection and replication of virulent virus.

Sign in / Sign up

Export Citation Format

Share Document