scholarly journals REFINING SAMPLING PROTOCOLS FOR INVENTORYING INVERTEBRATE BIODIVERSITY: INFLUENCE OF DRIFT-FENCE LENGTH AND PITFALL TRAP DIAMETER ON SPIDERS

2005 ◽  
Vol 33 (3) ◽  
pp. 681-702 ◽  
Author(s):  
Karl E. C. Brennan ◽  
Jonathan D. Majer ◽  
Melinda L. Moir
Keyword(s):  
Pitfall Trap ◽  
Invertebrate Biodiversity ◽  
Drift Fence ◽  
Sampling Protocols

Optimising design and effort for environmental surveys using dung beetles (Coleoptera: Scarabaeidae)

2016 ◽  
Vol 149 (2) ◽  
pp. 214-226 ◽  
Author(s):  
Claudia Tocco ◽  
Danielle E.A. Quinn ◽  
John M. Midgley ◽  
Martin H. Villet
Keyword(s):  
Sampling Method ◽  
Ground Level ◽  
Dung Beetle ◽  
Pitfall Trap ◽  
Dung Beetles ◽  
Sampling Effort ◽  
Eastern Cape ◽  
Trap Design ◽  
Sampling Protocols ◽  
By Catch

AbstractIn biological monitoring, deploying an effective standardised quantitative sampling method, optimised by trap design and sampling effort, is an essential consideration. To exemplify this using dung beetle (Coleoptera: Scarabaeidae: Scarabaeinae and Aphodiinae) communities, three pitfall trap designs (un-baited (TN), baited at ground level (flat trap, TF), and baited above the trap (hanging trap, TH)), employed with varying levels of sampling effort (number of traps=1, 2, 3 … 10; number of days=1, 2, 3), were evaluated for sampling completeness and efficiency in the Eastern Cape, South Africa. Modelling and resampling simulation approaches were used to suggest optimal sampling protocols across environmentally diverse sites. Overall, TF recovered the greatest abundance and species richness of dung beetles, but behavioural guilds showed conflicting trends: endocoprids preferred TH while paracoprids and telocoprids preferred TF. Resampling simulation of trap type and the two components of sampling effort suggested that six TF traps left for three days was most efficient in obtaining a representative sample and allowed differentiation between trap types, allowing the improved efficiency to be recognised. The effect of trap type on non-target specimens, particularly ants, was also investigated. TF and TH caught almost no by-catch, which is ethically desirable.

scholarly journals AMPHIBIAN AND REPTILE COLONIZATION OF RECLAIMED COAL SPOIL GRASSLANDS

2014 ◽  
pp. 59-68 ◽  
Author(s):  
Michael J. Lannoo ◽  
Vanessa C.K. Terrell ◽  
Jaimie L. Klemish ◽  
Nathan J. Engbrecht ◽  
John A. May ◽  
...  
Keyword(s):  
Habitat Loss ◽  
Pitfall Trap ◽  
Major Malformation ◽  
Research Activities ◽  
Drift Fence ◽  
Reptile Species ◽  
Managed Landscapes ◽  
Industrial Landscapes ◽  
Post Industrial

While habitat loss is a major driver of amphibian and reptile declines globally, a subset of post-industrial landscapes, reclaimed and restored, are creating habitat for these animals. In a previous work, we showed that amphibians and reptiles use reclaimed and restored grasslands. In the present work we quantify captures at drift-fence/pitfall trap arrays over two consecutive years and show that several species of amphibians are not only successfully reproducing but that juveniles are being recruited into the population. In particular, 15,844 amphibians and 334 reptiles representing 25 species (14 amphibians, 11 reptiles) were captured at drift fences in 2009 and 2010. Nine additional reptile species were found opportunistically while conducting other research activities at the study site. Out of a total of 8,064 metamorphosing juveniles we detected 126 malformations, a 1.6% rate. The major malformation types were limbs missing (amelia) or foreshortened (ectromely), eye discolorations, and digits foreshortened (ectrodactyly) or small (brachydactyly). Our data show that reclaimed, restored, and properly managed landscapes can support reproducing populations of amphibians and reptiles with low malformation rates, including species in decline across other portions of their range.

Reduced sampling protocols in estimation of insulin sensitivity and glucose effectiveness using the minimal model in NIDDM

Diabetes ◽  
1993 ◽  
Vol 42 (11) ◽  
pp. 1635-1641 ◽  
Author(s):  
P. A. Coates ◽  
R. L. Ollerton ◽  
S. D. Luzio ◽  
I. S. Ismail ◽  
D. R. Owens
Keyword(s):  
Insulin Sensitivity ◽  
Minimal Model ◽  
Glucose Effectiveness ◽  
Sampling Protocols

scholarly journals Importance of between and within Subject Variability in Extracellular Vesicle Abundance and Cargo when Performing Biomarker Analyses

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 485
Author(s):  
Lauren A. Newman ◽  
Alia Fahmy ◽  
Michael J. Sorich ◽  
Oliver G. Best ◽  
Andrew Rowland ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Drug Exposure ◽  
Extracellular Vesicle ◽  
The Novel ◽  
Protein Markers ◽  
Diurnal Variability ◽  
Normal Ranges ◽  
Sampling Protocols ◽  
Subject Variability ◽  
Within Subject Variability

Small extracellular vesicles (sEV) have emerged as a potential rich source of biomarkers in human blood and present the intriguing potential for a ‘liquid biopsy’ to track disease and the effectiveness of interventions. Recently, we have further demonstrated the potential for EV derived biomarkers to account for variability in drug exposure. This study sought to evaluate the variability in abundance and cargo of global and liver-specific circulating sEV, within (diurnal) and between individuals in a cohort of healthy subjects (n = 10). We present normal ranges for EV concentration and size and expression of generic EV protein markers and the liver-specific asialoglycoprotein receptor 1 (ASGR1) in samples collected in the morning and afternoon. EV abundance and cargo was generally not affected by fasting, except CD9 which exhibited a statistically significant increase (p = 0.018). Diurnal variability was observed in the expression of CD81 and ASGR1, which significantly decreased (p = 0.011) and increased (p = 0.009), respectively. These results have potential implications for study sampling protocols and normalisation of biomarker data when considering the expression of sEV derived cargo as a biomarker strategy. Specifically, the novel finding that liver-specific EVs exhibit diurnal variability in healthy subjects should have broad implications in the study of drug metabolism and development of minimally invasive biomarkers for liver disease.

Diet composition of Steller sea lions (Eumetopias jubatus) in Frederick Sound, southeast Alaska: a comparison of quantification methods using scats to describe temporal and spatial variabilities

2015 ◽  
Vol 93 (5) ◽  
pp. 361-376 ◽  
Author(s):  
D.J. Tollit ◽  
M.A. Wong ◽  
A.W. Trites
Keyword(s):  
Pacific Salmon ◽  
Biomass Energy ◽  
Eumetopias Jubatus ◽  
Steller Sea Lions ◽  
Southeast Alaska ◽  
Merluccius Productus ◽  
Sea Lions ◽  
Sampling Protocols ◽  
Dietary Indices

We compared eight dietary indices used to describe the diet of Steller sea lions (Eumetopias jubatus (Schreber, 1776)) from 2001 to 2004 in Frederick Sound, southeast Alaska. Remains (n = 9666 items) from 59+ species categories were identified from 1684 fecal samples (scats) from 14 collection periods. The most frequently occurring prey were walleye pollock (Theragra chalcogramma (Pallas, 1814) = Gadus chalcogrammus Pallas, 1814; 95%), Pacific herring (Clupea pallasii Valenciennes in Cuvier and Valenciennes, 1847; 30%), Pacific hake (Merluccius productus (Ayres, 1855); 29%), and arrowtooth flounder (Atheresthes stomias (Jordan and Gilbert, 1880) = Reinhardtius stomias (Jordan and Gilbert, 1880); 21%). These species, along with Pacific salmon (genus Oncorhynchus Suckley, 1861) and skate (genus Raja L., 1758), accounted for 80%–90% of the reconstructed biomass and energy contribution, with pollock contributing 37%–60%. Overall, 80% of fish were 14–42 cm long and mainly pelagic, though 40% of scats contained benthic-associated prey. Steller sea lions switched from adult pollock to strong cohorts of juvenile pollock, and took advantage of spawning concentrations of salmon in autumn and herring in late spring and summer, as well as a climate-driven increase in hake availability. Observed temporal and site differences in diet confirm the need for robust long-term scat sampling protocols. All major indices similarly tracked key temporal changes, despite differences in occurrence and biomass-energy-based diet estimates linked to prey size and energy-density effects and the application of correction factors.

scholarly journals An improved computational method to assess pituitary responsiveness to secretagogue stimuli

2002 ◽  
pp. 323-332 ◽  
Author(s):  
A Sartorio ◽  
G De Nicolao ◽  
D Liberati
Keyword(s):  
Measurement Error ◽  
Measurement Errors ◽  
Pituitary Hormones ◽  
Computational Method ◽  
Kinetics Parameters ◽  
Thyrotrophin Releasing Hormone ◽  
Deconvolution Analysis ◽  
Releasing Hormone ◽  
Sampling Protocols ◽  
Peak Value

OBJECTIVE: The quantitative assessment of gland responsiveness to exogenous stimuli is typically carried out using the peak value of the hormone concentrations in plasma, the area under its curve (AUC), or through deconvolution analysis. However, none of these methods is satisfactory, due to either sensitivity to measurement errors or various sources of bias. The objective was to introduce and validate an easy-to-compute responsiveness index, robust in the face of measurement errors and interindividual variability of kinetics parameters. DESIGN: The new method has been tested on responsiveness tests for the six pituitary hormones (using GH-releasing hormone, thyrotrophin-releasing hormone, gonadotrophin-releasing hormone and corticotrophin-releasing hormone as secretagogues), for a total of 174 tests. Hormone concentrations were assayed in six to eight samples between -30 min and 120 min from the stimulus. METHODS: An easy-to-compute direct formula has been worked out to assess the 'stimulated AUC', that is the part of the AUC of the response curve depending on the stimulus, as opposed to pre- and post-stimulus spontaneous secretion. The weights of the formula have been reported for the six pituitary hormones and some popular sampling protocols. RESULTS AND CONCLUSIONS: The new index is less sensitive to measurement error than the peak value. Moreover, it provides results that cannot be obtained from a simple scaling of either the peak value or the standard AUC. Future studies are needed to show whether the reduced sensitivity to measurement error and the proportionality to the amount of released hormone render the stimulated AUC indeed a valid alternative to the peak value for the diagnosis of the different pathophysiological states, such as, for instance, GH deficits.

scholarly journals Evaluation of Two Surface Sampling Methods for Detection of Erwinia herbicola on a Variety of Materials by Culture and Quantitative PCR

2007 ◽  
Vol 73 (11) ◽  
pp. 3505-3510 ◽  
Author(s):  
Mark P. Buttner ◽  
Patricia Cruz ◽  
Linda D. Stetzenbach ◽  
Tracy Cronin
Keyword(s):  
Quantitative Pcr ◽  
Sampling Methods ◽  
Collection Efficiency ◽  
Test Chamber ◽  
Surface Sampling ◽  
Laminate Glass ◽  
Erwinia Herbicola ◽  
Surface Samples ◽  
Sampling Protocols ◽  
Surface Materials

ABSTRACT This research was designed to evaluate surface sampling protocols for use with culture and quantitative PCR (QPCR) amplification assay for detection of the gram-negative bacterial biothreat simulant Erwinia herbicola on a variety of surface materials. Surfaces selected for evaluation were wood laminate, glass and computer monitor screens, metal file cabinets, plastic arena seats, nylon seat cushions, finished concrete flooring, and vinyl tile flooring. Laboratory and test chamber studies were performed to evaluate two sampling methods, a sponge and a macrofoam swab, for detection of E. herbicola on surface materials. In laboratory trials, seven materials were inoculated with a known concentration of E. herbicola cells and samples were collected from the surfaces of the materials to determine sampling efficiencies. Culture analysis was ineffective for assessing E. herbicola collection efficiency because very few culturable cells were obtained from surface samples. QPCR demonstrated that E. herbicola DNA was present in high concentrations on all of the surface samples, and sampling efficiencies ranged from 0.7 to 52.2%, depending on the sampling method and the surface material. The swab was generally more efficient than the sponge for collection of E. herbicola from surfaces. Test chamber trials were also performed in which E. herbicola was aerosolized into the chamber and allowed to settle onto test materials. Surface sampling results supported those obtained in laboratory trials. The results of this study demonstrate the capabilities of QPCR to enhance the detection and enumeration of biocontaminants on surface materials and provide information on the comparability of sampling methods.

THE EFFECT OF SOD TYPE ON THE OCCURRENCE OF GROUND BEETLES (COLEOPTERA: CARABIDAE) IN A PEST MANAGEMENT APPLE ORCHARD

1984 ◽  
Vol 116 (2) ◽  
pp. 165-171 ◽  
Author(s):  
N. J. Holliday ◽  
E. A. C. Hagley
Keyword(s):  
Pest Management ◽  
Pitfall Trap ◽  
Common Species ◽  
Ground Beetles ◽  
Apple Orchard ◽  
Soil Samples ◽  
Soil Sampling ◽  
Pitfall Trapping ◽  
Before And After ◽  
Trap Catches

AbstractThe effects on carabids of natural, fescue, and rye sod types and of tillage were investigated in a pest management apple orchard. Carabids were sampled before and after the treatments by pitfall trapping and by two types of soil sampling. There were no significant effects of sod type on pitfall trap catches; however the abundance of all common species in soil samples was significantly affected by sod types. Usually in soil samples carabids were most abundant in natural sod and least abundant in tilled plots; numbers were intermediate in fescue and rye. Sod type did not affect structure or diversity of the carabid fauna.

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