scholarly journals Optimized Flow Cytometric Analysis of Central Nervous System Tissue Reveals Novel Functional Relationships Among Cells Expressing CD133, CD15, and CD24

Stem Cells ◽  
2007 ◽  
Vol 25 (6) ◽  
pp. 1560-1570 ◽  
Author(s):  
David M. Panchision ◽  
Hui-Ling Chen ◽  
Francesca Pistollato ◽  
Daniela Papini ◽  
Hsiao-Tzu Ni ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Flow Cytometric Analysis ◽  
Central Nervous System Tissue ◽  
Functional Relationships ◽  
Flow Cytometric

Flow cytometric sorting of neuronal and glial nuclei from central nervous system tissue

2010 ◽  
Vol 226 (2) ◽  
pp. 552-558 ◽  
Author(s):  
Seiji Okada ◽  
Hirokazu Saiwai ◽  
Hiromi Kumamaru ◽  
Kensuke Kubota ◽  
Akihito Harada ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Central Nervous System Tissue ◽  
Flow Cytometric ◽  
Flow Cytometric Sorting

Multiparameter flow cytometric analysis of neoplasms of the central nervous system

Neurosurgery ◽  
1990 ◽  
pp. 83 ◽  
Author(s):  
A J Appley ◽  
P L Fitzgibbons ◽  
P T Chandrasoma ◽  
D R Hinton ◽  
M L Apuzzo
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
The Central Nervous System

In situ cell kinetics studies on human neuroectodermal tumors with bromodeoxyuridine labeling

1986 ◽  
Vol 64 (3) ◽  
pp. 453-459 ◽  
Author(s):  
Takao Hoshino ◽  
Tadashi Nagashima ◽  
Judith A. Murovic ◽  
Charles B. Wilson ◽  
Michael S. B. Edwards ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Single Cell ◽  
Flow Cytometric Analysis ◽  
S Phase ◽  
Cell Suspensions ◽  
Phase Fraction ◽  
Flow Cytometric ◽  
Neuroectodermal Tumors

✓ Thirty-eight patients undergoing surgical removal of neuroectodermal tumors of the central nervous system were given a 1-hour intravenous infusion of bromodeoxyuridine (BUdR), 150 to 200 mg/sq m, to label tumor cells in the deoxyribonucleic acid (DNA) synthesis phase (S-phase). The excised tumor specimens were divided into two portions: one was fixed with 70% ethanol and embedded in paraffin and the other was digested with an enzyme cocktail to make a single-cell suspension. The paraffin-embedded tissues were stained by an indirect peroxidase method using anti-BUdR monoclonal antibody (MA) as the first antibody. Single-cell suspensions were reacted with fluorescein isothiocyanate (FITC)-conjugated anti-BUdR MA's for flow cytometric analysis. S-phase cells that had incorporated BUdR into their DNA were well stained by both methods. The percentage of BUdR-labeled cells, or S-phase fraction, was calculated in tissue sections by microscopic examination and in single-cell suspensions by flow cytometric analysis. The biological malignancy of the tumors was reflected in the S-phase fractions, which were 5% to 20% for glioblastoma multiforme, medulloblastoma, and highly anaplastic astrocytoma, but less than 1% in most moderately anaplastic astrocytomas, ependymomas, and mixed gliomas. Two juvenile pilocytic astrocytomas and two low-grade astrocytomas from children had high S-phase fractions despite the fairly benign and slow-growing nature of these tumors. These results indicate that the S-phase fraction obtained immunocytochemically with anti-BUdR MA's may provide useful information in estimating the biological malignancy of human central nervous system tumors in situ.

Confocal laser microscopy of impregnated central nervous system tissue

1988 ◽  
Vol 46 ◽  
pp. 94-95
Author(s):  
J.N. Turner ◽  
M. Siemens ◽  
D. Szarowski ◽  
D.N. Collins
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Confocal Laser ◽  
High Contrast ◽  
Central Nervous System Tissue ◽  
Tissue Samples ◽  
Reflected Light

A classic preparation of central nervous system tissue (CNS) is the Golgi procedure popularized by Cajal. The method is partially specific as only a few cells are impregnated with silver chromate usualy after osmium post fixation. Samples are observable by light (LM) or electron microscopy (EM). However, the impregnation is often so dense that structures are masked in EM, and the osmium background may be undesirable in LM. Gold toning is used for a subtle but high contrast EM preparation, and osmium can be omitted for LM. We are investigating these preparations as part of a study to develop correlative LM and EM (particularly HVEM) methodologies in neurobiology. Confocal light microscopy is particularly useful as the impregnated cells have extensive three-dimensional structure in tissue samples from one to several hundred micrometers thick. Boyde has observed similar preparations in the tandem scanning reflected light microscope (TSRLM).

HISTOPATHOLOGY OF MULTIPLE SCLEROSIS LESIONS DETECTED BY MAGNETIC RESONANCE IMAGING IN UNFIXED POSTMORTEM CENTRAL NERVOUS SYSTEM TISSUE

Brain ◽  
1991 ◽  
Vol 114 (2) ◽  
pp. 1013-1023 ◽  
Author(s):  
J. NEWCOMBE ◽  
C. P. HAWKINS ◽  
C. L. HENDERSON ◽  
H. A. PATEL ◽  
M. N. WOODROOFE ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Multiple Sclerosis ◽  
Central Nervous System ◽  
Nervous System ◽  
Magnetic Resonance ◽  
Resonance Imaging ◽  
Central Nervous System Tissue

Rapid test for the preclinical postmortem diagnosis of BSE in central nervous system tissue

2001 ◽  
Vol 149 (19) ◽  
pp. 577-582 ◽  
Author(s):  
J. Grassi ◽  
S. Simon ◽  
C. Crminon ◽  
Y. Frobert ◽  
E. Comoy ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Rapid Test ◽  
Central Nervous System Tissue ◽  
Postmortem Diagnosis
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