Flow cytometric sorting of neuronal and glial nuclei from central nervous system tissue

2010 ◽  
Vol 226 (2) ◽  
pp. 552-558 ◽  
Author(s):  
Seiji Okada ◽  
Hirokazu Saiwai ◽  
Hiromi Kumamaru ◽  
Kensuke Kubota ◽  
Akihito Harada ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Central Nervous System Tissue ◽  
Flow Cytometric ◽  
Flow Cytometric Sorting

scholarly journals Optimized Flow Cytometric Analysis of Central Nervous System Tissue Reveals Novel Functional Relationships Among Cells Expressing CD133, CD15, and CD24

Stem Cells ◽  
2007 ◽  
Vol 25 (6) ◽  
pp. 1560-1570 ◽  
Author(s):  
David M. Panchision ◽  
Hui-Ling Chen ◽  
Francesca Pistollato ◽  
Daniela Papini ◽  
Hsiao-Tzu Ni ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Flow Cytometric Analysis ◽  
Central Nervous System Tissue ◽  
Functional Relationships ◽  
Flow Cytometric

Confocal laser microscopy of impregnated central nervous system tissue

1988 ◽  
Vol 46 ◽  
pp. 94-95
Author(s):  
J.N. Turner ◽  
M. Siemens ◽  
D. Szarowski ◽  
D.N. Collins
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Confocal Laser ◽  
High Contrast ◽  
Central Nervous System Tissue ◽  
Tissue Samples ◽  
Reflected Light

A classic preparation of central nervous system tissue (CNS) is the Golgi procedure popularized by Cajal. The method is partially specific as only a few cells are impregnated with silver chromate usualy after osmium post fixation. Samples are observable by light (LM) or electron microscopy (EM). However, the impregnation is often so dense that structures are masked in EM, and the osmium background may be undesirable in LM. Gold toning is used for a subtle but high contrast EM preparation, and osmium can be omitted for LM. We are investigating these preparations as part of a study to develop correlative LM and EM (particularly HVEM) methodologies in neurobiology. Confocal light microscopy is particularly useful as the impregnated cells have extensive three-dimensional structure in tissue samples from one to several hundred micrometers thick. Boyde has observed similar preparations in the tandem scanning reflected light microscope (TSRLM).

HISTOPATHOLOGY OF MULTIPLE SCLEROSIS LESIONS DETECTED BY MAGNETIC RESONANCE IMAGING IN UNFIXED POSTMORTEM CENTRAL NERVOUS SYSTEM TISSUE

Brain ◽  
1991 ◽  
Vol 114 (2) ◽  
pp. 1013-1023 ◽  
Author(s):  
J. NEWCOMBE ◽  
C. P. HAWKINS ◽  
C. L. HENDERSON ◽  
H. A. PATEL ◽  
M. N. WOODROOFE ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Multiple Sclerosis ◽  
Central Nervous System ◽  
Nervous System ◽  
Magnetic Resonance ◽  
Resonance Imaging ◽  
Central Nervous System Tissue

Rapid test for the preclinical postmortem diagnosis of BSE in central nervous system tissue

2001 ◽  
Vol 149 (19) ◽  
pp. 577-582 ◽  
Author(s):  
J. Grassi ◽  
S. Simon ◽  
C. Crminon ◽  
Y. Frobert ◽  
E. Comoy ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Rapid Test ◽  
Central Nervous System Tissue ◽  
Postmortem Diagnosis

Control Points To Reduce Movement of Central Nervous System Tissue during Beef Slaughter

2017 ◽  
Vol 80 (2) ◽  
pp. 355-360
Author(s):  
J. L. Aalhus ◽  
R. D. Thacker ◽  
I. L. Larsen ◽  
J. C. Roberts ◽  
M. A. Price ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cold Water ◽  
Effective Means ◽  
Hot Water ◽  
Spongiform Encephalopathy ◽  
Cross Contamination ◽  
Central Nervous System Tissue ◽  
Before And After ◽  
Disease Variant

ABSTRACT Consumption of central nervous system tissue (CNST) from cattle with bovine spongiform encephalopathy (BSE) is thought to cause the human neurological disease, variant Creutzfeldt-Jacob disease. To identify points of cross-contamination of beef carcasses with CNST, 55 young beef cattle were slaughtered and processed through a federally inspected multispecies abattoir. The objectives of this study were to evaluate CNST spread following the placement of a plug in the penetration site of the skull after captive bolt stunning, to evaluate cross-contamination of carcasses before and after splitting, to compare the effects of hot water pasteurization (84°C for 10 s) versus cold water wash (10°C for 30 s) for reducing CNST on the carcass, and to examine other possible sources of cross-contamination in the abattoir. Results indicated that the use of a plastic plug reduced CNST contamination near the bolt penetration site. This study also confirmed that carcass splitting resulted in an increase in CNST contamination at various areas of the carcass. Hot water pasteurization appeared to be an effective means of removing CNST contamination from carcasses in most of the areas sampled.

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