scholarly journals Applications of smartphone-based near-infrared (NIR) imaging, measurement, and spectroscopy technologies to point-of-care (POC) diagnostics

2021 ◽  
Vol 22 (3) ◽  
pp. 171-189
Author(s):  
Wenjing Huang ◽  
Shenglin Luo ◽  
Dong Yang ◽  
Sheng Zhang
Keyword(s):  
Near Infrared ◽  
Point Of Care ◽  
Nir Imaging

scholarly journals Recent Progress on Near-Infrared Photoacoustic Imaging: Imaging Modality and Organic Semiconducting Agents

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1693 ◽  
Author(s):  
Doyoung Jung ◽  
Suhyeon Park ◽  
Changho Lee ◽  
Hyungwoo Kim
Keyword(s):  
Recent Progress ◽  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Imaging Modality ◽  
Point Of Care ◽  
Tissue Imaging ◽  
Nir Imaging ◽  
Precise Diagnosis ◽  
Deep Tissue Imaging ◽  
Review Reports

Over the past few decades, the photoacoustic (PA) effect has been widely investigated, opening up diverse applications, such as photoacoustic spectroscopy, estimation of chemical energies, or point-of-care detection. Notably, photoacoustic imaging (PAI) has also been developed and has recently received considerable attention in bio-related or clinical imaging fields, as it now facilitates an imaging platform in the near-infrared (NIR) region by taking advantage of the significant advancement of exogenous imaging agents. The NIR PAI platform now paves the way for high-resolution, deep-tissue imaging, which is imperative for contemporary theragnosis, a combination of precise diagnosis and well-timed therapy. This review reports the recent progress on NIR PAI modality, as well as semiconducting contrast agents, and outlines the trend in current NIR imaging and provides further direction for the prospective development of PAI systems.

The Role of NIR Fluorescence in MDR Cancer Treatment: From Targeted Imaging to Phototherapy

2020 ◽  
Vol 27 (33) ◽  
pp. 5510-5529
Author(s):  
Zengtao Wang ◽  
Qingqing Meng ◽  
Shaoshun Li
Keyword(s):  
Cancer Treatment ◽  
Near Infrared ◽  
Combined Therapy ◽  
Efflux Pumps ◽  
Cross Resistance ◽  
Great Promise ◽  
Drug Efflux ◽  
Nir Dyes ◽  
Nir Imaging ◽  
Drug Efflux Pumps

Background: Multidrug Resistance (MDR) is defined as a cross-resistance of cancer cells to various chemotherapeutics and has been demonstrated to correlate with drug efflux pumps. Visualization of drug efflux pumps is useful to pre-select patients who may be insensitive to chemotherapy, thus preventing patients from unnecessary treatment. Near-Infrared (NIR) imaging is an attractive approach to monitoring MDR due to its low tissue autofluorescence and deep tissue penetration. Molecular NIR imaging of MDR cancers requires stable probes targeting biomarkers with high specificity and affinity. Objective: This article aims to provide a concise review of novel NIR probes and their applications in MDR cancer treatment. Results: Recently, extensive research has been performed to develop novel NIR probes and several strategies display great promise. These strategies include chemical conjugation between NIR dyes and ligands targeting MDR-associated biomarkers, native NIR dyes with inherent targeting ability, activatable NIR probes as well as NIR dyes loaded nanoparticles. Moreover, NIR probes have been widely employed for photothermal and photodynamic therapy in cancer treatment, which combine with other modalities to overcome MDR. With the rapid advancing of nanotechnology, various nanoparticles are incorporated with NIR dyes to provide multifunctional platforms for controlled drug delivery and combined therapy to combat MDR. The construction of these probes for MDR cancers targeted NIR imaging and phototherapy will be discussed. Multimodal nanoscale platform which integrates MDR monitoring and combined therapy will also be encompassed. Conclusion: We believe these NIR probes project a promising approach for diagnosis and therapy of MDR cancers, thus holding great potential to reach clinical settings in cancer treatment.

scholarly journals Performance Evaluation of Dorsal Vein Network of Hand Imaging Using Relative Total Variation-Based Regularization for Smoothing Technique in a Miniaturized Vein Imaging System: A Pilot Study

2021 ◽  
Vol 18 (4) ◽  
pp. 1548 ◽  
Author(s):  
Kyuseok Kim ◽  
Hyun-Woo Jeong ◽  
Youngjin Lee
Keyword(s):  
Image Quality ◽  
Total Variation ◽  
Near Infrared ◽  
Imaging System ◽  
Histogram Equalization ◽  
Evaluation Performance ◽  
Visual Evaluation ◽  
Évaluation Performance ◽  
Nir Imaging ◽  
Relative Total Variation

Vein puncture is commonly used for blood sampling, and accurately locating the blood vessel is an important challenge in the field of diagnostic tests. Imaging systems based on near-infrared (NIR) light are widely used for accurate human vein puncture. In particular, segmentation of a region of interest using the obtained NIR image is an important field, and research for improving the image quality by removing noise and enhancing the image contrast is being widely conducted. In this paper, we propose an effective model in which the relative total variation (RTV) regularization algorithm and contrast-limited adaptive histogram equalization (CLAHE) are combined, whereby some major edge information can be better preserved. In our previous study, we developed a miniaturized NIR imaging system using light with a wavelength of 720–1100 nm. We evaluated the usefulness of the proposed algorithm by applying it to images acquired by the developed NIR imaging system. Compared with the conventional algorithm, when the proposed method was applied to the NIR image, the visual evaluation performance and quantitative evaluation performance were enhanced. In particular, when the proposed algorithm was applied, the coefficient of variation was improved by a factor of 15.77 compared with the basic image. The main advantages of our algorithm are the high noise reduction efficiency, which is beneficial for reducing the amount of undesirable information, and better contrast. In conclusion, the applicability and usefulness of the algorithm combining the RTV approach and CLAHE for NIR images were demonstrated, and the proposed model can achieve a high image quality.

Development of a Novel SNAP-Epitope Tag/Near-Infrared Imaging Assay to Quantify G Protein-Coupled Receptor Degradation in Human Cells

2021 ◽  
pp. 247255522097979
Author(s):  
Kyung-Soon Lee ◽  
Edelmar Navaluna ◽  
Nicole M. Marsh ◽  
Eric M. Janezic ◽  
Chris Hague
Keyword(s):  
G Protein ◽  
Adrenergic Receptor ◽  
Near Infrared ◽  
Human Cells ◽  
Receptor Subtypes ◽  
G Protein Coupled Receptor ◽  
Functional Responses ◽  
Epitope Tag ◽  
Nir Imaging ◽  
G Protein Coupled

We have developed a novel reporter assay that leverages SNAP-epitope tag/near-infrared (NIR) imaging technology to monitor G protein-coupled receptor (GPCR) degradation in human cell lines. N-terminal SNAP-tagged GPCRs were subcloned and expressed in human embryonic kidney (HEK) 293 cells and then subjected to 24 h of cycloheximide (CHX)-chase degradation assays to quantify receptor degradation half-lives ( t1/2) using LICOR NIR imaging–polyacrylamide gel electrophoresis (PAGE) analysis. Thus far, we have used this method to quantify t1/2 for all nine adrenergic (ADRA1A, ADRA1B, ADRA1D, ADRA2A, ADRA2B, ADRA2C, ADRB1, ADRB2, ADRB3), five somatostatin (SSTR1, SSTR2, SSTR3, SSTR4, SSTR5), four chemokine (CXCR1, CXCR2, CXCR3, CXCR5), and three 5-HT2 (5HT2A, 5HT2B, 5HT2C) receptor subtypes. SNAP-GPCR-CHX degradation t1/2 values ranged from 0.52 h (ADRA1D) to 5.5 h (SSTR3). On the contrary, both the SNAP-tag alone and SNAP-tagged and endogenous β-actin were resistant to degradation with CHX treatment. Treatment with the proteasome inhibitor bortezomib produced significant but variable increases in SNAP-GPCR protein expression levels, indicating that SNAP-GPCR degradation primarily occurs through the proteasome. Remarkably, endogenous β2-adrenergic receptor/ADRB2 dynamic mass redistribution functional responses to norepinephrine were significantly decreased following CHX treatment, with a time course equivalent to that observed with the SNAP-ADRB2 degradation assay. We subsequently adapted this assay into a 96-well glass-bottom plate format to facilitate high-throughput GPCR degradation screening. t1/2 values quantified for the α1-adrenergic receptor subtypes (ADRA1A, ADRA1B, ADR1D) using the 96-well-plate format correlated with t1/2 values quantified using NIR-PAGE imaging analysis. In summary, this novel assay permits precise quantitative analysis of GPCR degradation in human cells and can be readily adapted to quantify degradation for any membrane protein of interest.

A Comparison of Near-Infrared Imaging and Computerized Tomography Scan for Detecting Maxillary Sinusitis

2021 ◽  
pp. 000348942110606
Author(s):  
Mehdi Abouzari ◽  
Brooke Sarna ◽  
Joon You ◽  
Adwight Risbud ◽  
Kotaro Tsutsumi ◽  
...  
Keyword(s):  
Maxillary Sinus ◽  
Computerized Tomography ◽  
Sensitivity And Specificity ◽  
Near Infrared ◽  
Maxillary Sinusitis ◽  
Tertiary Care ◽  
Pixel Intensity ◽  
Sinus Disease ◽  
Nir Imaging ◽  
Average Pixel Intensity

Objective: To investigate the use of near-infrared (NIR) imaging as a tool for outpatient clinicians to quickly and accurately assess for maxillary sinusitis and to characterize its accuracy compared to computerized tomography (CT) scan. Methods: In a prospective investigational study, NIR and CT images from 65 patients who presented to a tertiary care rhinology clinic were compared to determine the sensitivity and specificity of NIR as an imaging modality. Results: The sensitivity and specificity of NIR imaging in distinguishing normal versus maxillary sinus disease was found to be 90% and 84%, normal versus mild maxillary sinus disease to be 76% and 91%, and mild versus severe maxillary sinus disease to be 96% and 81%, respectively. The average pixel intensity was also calculated and compared to the modified Lund-Mackay scores from CT scans to assess the ability of NIR imaging to stratify the severity of maxillary sinus disease. Average pixel intensity over a region of interest was significantly different ( P < .001) between normal, mild, and severe disease, as well as when comparing normal versus mild ( P < .001, 95% CI 42.22-105.39), normal versus severe ( P < .001, 95% CI 119.43-174.14), and mild versus severe ( P < .001, 95% CI 41.39-104.56) maxillary sinus disease. Conclusion: Based on this data, NIR shows promise as a tool for identifying patients with potential maxillary sinus disease as well as providing information on severity of disease that may guide administration of appropriate treatments.

scholarly journals Imaging of β-amyloid plaques by near infrared fluorescent tracers: a new frontier for chemical neuroscience

2015 ◽  
Vol 44 (7) ◽  
pp. 1807-1819 ◽  
Author(s):  
Matteo Staderini ◽  
María Antonia Martín ◽  
Maria Laura Bolognesi ◽  
J. Carlos Menéndez
Keyword(s):  
Near Infrared ◽  
Amyloid Plaques ◽  
Fluorescent Tracers ◽  
Non Invasive ◽  
Nir Imaging ◽  
New Frontier ◽  
Invasive Method ◽  
Β Amyloid

Near infrared (NIR) imaging is a promising and non-invasive method to visualize amyloid plaquesin vivo.

scholarly journals Recording microscale variations in snowpack layering using near-infrared photography

2010 ◽  
Vol 56 (195) ◽  
pp. 75-80 ◽  
Author(s):  
Ken D. Tape ◽  
Nick Rutter ◽  
Hans-Peter Marshall ◽  
Richard Essery ◽  
Matthew Sturm
Keyword(s):  
Near Infrared ◽  
Digital Camera ◽  
Post Processing ◽  
Snow Layer ◽  
Avalanche Forecasting ◽  
The North ◽  
Nir Imaging ◽  
Snow Stratigraphy ◽  
Wind Events ◽  
Lateral Variations

AbstractDeposition of snow from precipitation and wind events creates layering within seasonal snowpacks. The thickness and horizontal continuity of layers within seasonal snowpacks can be highly variable, due to snow blowing around topography and vegetation, and this has important implications for hydrology, remote sensing and avalanche forecasting. In this paper, we present practical field and post-processing protocols for recording lateral variations in snow stratigraphy using near-infrared (NIR) photography. A Fuji S9100 digital camera, modified to be sensitive to NIR wavelengths, was mounted on a rail system that allowed for rapid imaging of a 10 m long snow trench excavated on the north side of Toolik Lake, Alaska (68°38′ N, 149°36′ W). Post-processing of the images included removal of lens distortion and vignetting. A tape measure running along the base of the trench provided known locations (control points) that permitted scaling and georeferencing. Snow layer heights estimated from the NIR images compared well with manual stratigraphic measurements made at 0.2 m intervals along the trench (n = 357, R2 = 0.97). Considerably greater stratigraphic detail was captured by the NIR images than in the manually recorded profiles. NIR imaging of snow trenches using the described protocols is an efficient tool for quantifying continuous microscale variations in snow layers and associated properties.

scholarly journals X-Ray and Near-Infrared Spectroscopy of Dim X-Ray Point Sources Constituting the Galactic Ridge X-Ray Emission

2014 ◽  
Vol 1 (1) ◽  
pp. 222-226
Author(s):  
Kumiko Morihana ◽  
Masahiro Tsujimoto ◽  
Ken Ebisawa
Keyword(s):  
Near Infrared ◽  
Cataclysmic Variables ◽  
Point Sources ◽  
X Ray ◽  
Nir Imaging ◽  
Group A ◽  
Field L ◽  
Imaging And Spectroscopy ◽  
Group B ◽  
Thermal Sources

We present the results of X-ray and Near-Infrared observations of the Galactic Ridge X-ray Emission (GRXE). We extracted 2,002 X-ray point sources in the <em>Chandra</em> Bulge Field (l =0°.113, b = 1°.424) down to ~10<sup>-14.8</sup> ergscm<sup>-2</sup>s<sup>-1</sup> in 2-8 keV band with the longest observation (900 ks) of the GRXE. Based on X-ray brightness and hardness, we classied the X-ray point sources into three groups: A (hard), B (soft and broad spectrum), and C (soft and peaked spectrum). In order to know populations of the X-ray point sources, we carried out NIR imaging and spectroscopy observation. We identied 11% of X-ray point sources with NIR and extracted NIR spectra for some of them. Based on X-ray and NIR properties, we concluded that non-thermal sources in the group A are mostly active galactic nuclei and the thermal sources are mostly white dwarf binaries such as cataclysmic variables (CVs) and Pre-CVs. We concluded that the group B and C sources are X-ray active stars in flare and quiescence, respectively.

In-vivo near-infrared optical imaging of growing osteosarcoma cell lesions xenografted in mice: dual-channel quantitative evaluation of volume and mineralization

2011 ◽  
Vol 52 (9) ◽  
pp. 978-988 ◽  
Author(s):  
Hitoshi Nakayama ◽  
Tomoyuki Kawase ◽  
Kazuhiro Okuda ◽  
Larry F Wolff ◽  
Hiromasa Yoshie
Keyword(s):  
Optical Imaging ◽  
Near Infrared ◽  
Imaging Technique ◽  
Ex Vivo ◽  
Acquisition Time ◽  
Osteosarcoma Cell ◽  
Dual Channel ◽  
Nir Imaging ◽  
Ex Vivo Imaging

Background In a previous study using a rodent osteosarcoma-grafted rat model, in which cell-dependent mineralization was previously demonstrated to proportionally increase with growth, we performed a quantitative analysis of mineral deposit formation using 99mTc-HMDP and found some weaknesses, such as longer acquisition time and narrower dynamic ranges (i.e. images easily saturated). The recently developed near-infrared (NIR) optical imaging technique is expected to non-invasively evaluate changes in living small animals in a quantitative manner. Purpose To test the feasibility of NIR imaging with a dual-channel system as a better alternative for bone scintigraphy by quantitatively evaluating mineralization along with the growth of osteosarcoma lesions in a mouse-xenograft model. Material and Methods The gross volume and mineralization of osteosarcoma lesions were evaluated in living mice simultaneously with dual-channels by NIR dye-labeled probes, 2-deoxyglucose (DG) and pamidronate (OS), respectively. To verify these quantitative data, retrieved osteosarcoma lesions were then subjected to ex-vivo imaging, weighing under wet conditions, microfocus-computed tomography (μCT) analysis, and histopathological examination. Results Because of less scattering and no anatomical overlapping, as generally shown, specific fluorescence signals targeted to the osteosarcoma lesions could be determined clearly by ex-vivo imaging. These data were well positively correlated with the in-vivo imaging data ( r > 0.8, P < 0.02). Other good to excellent correlations ( r > 0.8, P < 0.02) were observed between DG accumulation and tumor gross volume and between OS accumulation and mineralization volume. Conclusion This in-vivo NIR imaging technique using DG and OS is sensitive to the level to simultaneously detect and quantitatively evaluate the growth and mineralization occuring in this type of osteosarcoma lesions of living mice without either invasion or sacrifice. By possible mutual complementation, this dual imaging system might be useful for accurate diagnosis even in the presence of overlapping tissues.

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