scholarly journals Scanning Electron Microscopy of Storage Cells filled with Dense Matrix. An application technique of an osmium digestion method to plant cells.

1995 ◽  
Vol 64 (2) ◽  
pp. 336-337
Author(s):  
Toshiaki MATSUDA ◽  
Hiromichi HARA ◽  
Kouichi KASHIWABA ◽  
Nobuo CHONAN
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Plant Cells ◽  
Dense Matrix ◽  
Digestion Method ◽  
Application Technique ◽  
Storage Cells ◽  
Scanning Electron

Scanning electron microscopy of plant cells using a variable-pressure SEM and cryogenic techniques

1993 ◽  
Vol 51 ◽  
pp. 260-261
Author(s):  
M. Yamada ◽  
K. Ueda ◽  
K. Kuboki ◽  
H. Matsushima ◽  
S. Joens
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Saturated Vapor ◽  
Plant Cells ◽  
Variable Pressure ◽  
Specimen Preparation ◽  
Operating Pressure ◽  
Vapor Pressures ◽  
Low Temperature Microscopy ◽  
Scanning Electron

Use of variable Pressure SEMs is spreading among electron microscopists The variable Pressure SEM does not necessarily require specimen Preparation such as fixation, dehydration, coating, etc which have been required for conventional scanning electron microscopy. The variable Pressure SEM allows operating Pressure of 1˜270 Pa in specimen chamber It does not allow microscopy of water-containing specimens under a saturated vapor Pressure of water. Therefore, it may cause shrink or deformation of water-containing soft specimens such as plant cells due to evaporation of water. A solution to this Problem is to lower the specimen temperature and maintain saturated vapor Pressures of water at low as shown in Fig. 1 On this technique, there is a Published report of experiment to have sufficient signal to noise ratio for scondary electron imaging at a relatively long working distance using an environmental SEM. We report here a new low temperature microscopy of soft Plant cells using a variable Pressure SEM (Hitachi S-225ON).

Mucilaginous film production by plant cells immobilised in a polyurethane or nylon matrix

1985 ◽  
Vol 63 (12) ◽  
pp. 2357-2363 ◽  
Author(s):  
M. J. C. Rhodes ◽  
R. J. Robins ◽  
R. J. Turner ◽  
J. I. Smith
Keyword(s):  
Thin Film ◽  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscope ◽  
Plant Cells ◽  
The Matrix ◽  
Nylon Fibre ◽  
Scanning Electron

The surface features of plant cells immobilised in a matrix of either reticulated polyurethane foam or nylon fibre have been examined with the scanning electron microscope. It has been found that both cells and matrix are enveloped in a thin film, the appearance of which is very dependent on the method by which material is prepared for scanning electron microscopy. The structure is severely damaged by fixation and dehydration. Only in specimens examined in the frozen hydrated state is a structure seen compatible with that observed with the light microscope. From the way the appearance of the film is affected by different methods of preparation for the scanning electron microscope, it is suggested that the film is a hydrated mucilage. The importance of this film for the retention of cells within the matrix is discussed.

scholarly journals A Simple Technique For The Removal Of Plant Cell Protoplasm To Facilitate Scanning : Electron Microscopy Of Fungal Haustoria And Plant Cell Wall Features

2001 ◽  
Vol 9 (3) ◽  
pp. 14-15 ◽  
Author(s):  
B. A. Richardson ◽  
C. W. Mims
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Wall ◽  
Plant Cell ◽  
Host Plants ◽  
Plant Cell Wall ◽  
Simple Technique ◽  
Plant Cells ◽  
Host Cell Wall ◽  
Scanning Electron

Several years ago Honegger (1985) described a simple technique for removing plant cell protoplasm in order to reveal details of interfaces between plant cells and fungal structures. This technique involves the use of Ariel a commercially available washing powder (Proctor and Gamble) containing a Bacillus substilis derived protease. We since have used this technique with excellent results to examine not only the morphology of fungal haustoria inside leaf cells of various host plants but also features of the inner surface of the host cell wall with scanning electron microscopy (SEM). Here we describe the procedure we have used to prepare samples for study and provide examples of the types of images we have obtained from our samples.

Field emission scanning electron microscopy of microtubule arrays in higher plant cells

PROTOPLASMA ◽  
1996 ◽  
Vol 195 (1-4) ◽  
pp. 168-182 ◽  
Author(s):  
Peter A. Vesk ◽  
Maret Vesk ◽  
Brian E. S. Gunning
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Field Emission ◽  
Plant Cells ◽  
Higher Plant ◽  
Scanning Electron

Methods for Viewing by Scanning Electron Microscopy the Interior Organization of Protoplasts of Plant Cells

1972 ◽  
Vol 30 ◽  
pp. 238-239 ◽  
Author(s):  
Walter J. Humphreys ◽  
Tomasz J. Wodzicki
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Liquid Nitrogen ◽  
Three Dimensional ◽  
Plant Cells ◽  
Osmic Acid ◽  
Razor Blade ◽  
Specimen Holder ◽  
Shallow Wells ◽  
Scanning Electron

The three-dimensional organization of protoplasts of plant cells may be studied by scanning electron microscopy by exposing the cell interiors by freeze-fracturing followed by freeze-drying. Tissue blocks (1 mm3) are double fixed in glutaraldehyde and osmic acid, washed in several changes of distilled water, and positioned in shallow wells drilled into the centers of specimen stubs used for scanning electron microscopy (Kent Cambridge type). Each specimen is frozen into place with a small droplet of water by plunging the specimen holder into liquid nitrogen. While under liquid nitrogen 0. 1-0. 2 mm of tissue left projecting out of the well is cut off level with the specimen holder surface by a single thrust of a pre-cooled razor blade.

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