scholarly journals Time course transcriptomic analysis of adipogenic differentiation of human preadipocytes isolated from subcutaneous white adipose tissue (WAT)

2016 ◽  
Author(s):  
SM Hartig
Keyword(s):  
Adipose Tissue ◽  
White Adipose Tissue ◽  
Time Course ◽  
Adipogenic Differentiation ◽  
Transcriptomic Analysis ◽  
Subcutaneous White Adipose Tissue ◽  
Human Preadipocytes

Acceptance of Murine Islet Allografts without Immunosuppression in the Inguinal Subcutaneous White Adipose Tissue Pretreated with bFGF

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 32-OR
Author(s):  
YUKI NAKAFUSA ◽  
NAOYOSHI NITTA ◽  
MASAFUNI NAKAMURA ◽  
YOHICHI YASUNAMI
Keyword(s):  
Adipose Tissue ◽  
White Adipose Tissue ◽  
Subcutaneous White Adipose Tissue ◽  
Islet Allografts

Irisin exerts dual effects on browning and adipogenesis of human white adipocytes

2016 ◽  
Vol 311 (2) ◽  
pp. E530-E541 ◽  
Author(s):  
Yuan Zhang ◽  
Chao Xie ◽  
Hai Wang ◽  
Robin M. Foss ◽  
Morgan Clare ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Uncoupling Protein ◽  
Adipogenic Differentiation ◽  
Mapk Signaling ◽  
Cellular Energy ◽  
Subcutaneous White Adipose Tissue ◽  
Basal Expression ◽  
White Adipocytes ◽  
Ucp1 Expression

To better understand the role of irisin in humans, we examined the effects of irisin in human primary adipocytes and fresh human subcutaneous white adipose tissue (scWAT). Human primary adipocytes derived from 28 female donors' fresh scWAT were used to examine the effects of irisin on browning and mitochondrial respiration, and preadipocytes were used to examine the effects of irisin on adipogenesis and osteogenesis. Cultured fragments of scWAT and perirenal brown fat were used for investigating signal transduction pathways that mediate irisin's browning effect by Western blotting to detect phosphorylated forms of p38, ERK, and STAT3 as well as uncoupling protein 1 (UCP1). Individual responses to irisin in scWAT were correlated with basal expression levels of brown/beige genes. Irisin upregulated the expression of browning-associated genes and UCP1 protein in both cultured primary mature adipocytes and fresh adipose tissues. It also significantly increased thermogenesis at 5 nmol/l by elevating cellular energy metabolism (OCR and ECAR). Treating human scWAT with irisin increased UCP1 expression by activating the ERK and p38 MAPK signaling. Blocking either pathway with specific inhibitors abolished irisin-induced UCP1 upregulation. However, our results showed that UCP1 in human perirenal adipose tissue was insensitive to irisin. Basal levels of brown/beige and FNDC5 genes correlated positively with the browning response of scWAT to irisin. In addition, irisin significantly inhibited adipogenic differentiation but promoted osteogenic differentiation. We conclude that irisin promotes “browning” of mature white adipocytes by increasing cellular thermogenesis, whereas it inhibits adipogenesis and promotes osteogenesis during lineage-specific differentiation. Our findings provide a rationale for further exploring the therapeutic use of irisin in obesity and exercise-associated bone formation.

Neonatal IL-4 exposure decreases adipogenesis of male rats into adulthood

2021 ◽  
Author(s):  
Tammy Ying ◽  
Thea N. Golden ◽  
Lan Cheng ◽  
Jeff Ishibashi ◽  
Patrick Seale ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Neonatal Period ◽  
Uncoupling Protein ◽  
Adipogenic Differentiation ◽  
Male Rats ◽  
Interleukin 4 ◽  
Sprague Dawley ◽  
Fat Cell ◽  
Subcutaneous White Adipose Tissue

The cytokine interleukin 4 (IL-4) can increase beige adipogenesis in adult rodents. However, neonatal animals use a distinct adipocyte precursor compartment for adipogenesis compared to adults. In this study, we address whether IL-4 can induce persistent effects on adipose tissue when administered subcutaneously in the interscapular region during the neonatal period in Sprague Dawley rats. We injected IL-4 into neonatal male rats during postnatal days 1-6, followed by analysis of adipose tissue and adipocyte precursors at 2 weeks and 10 weeks of age. Adipocyte precursors were cultured and subjected to differentiation in vitro. We found that a short and transient IL-4 exposure in neonates upregulated uncoupling protein 1 (Ucp1) mRNA expression and decreased fat cell size in subcutaneous white adipose tissue (WAT). Adipocyte precursors from mature rats that had been treated with IL-4 as neonates displayed a decrease in adiponectin (Adipoq) but no change in Ucp1 expression, as compared to controls. Thus, neonatal IL-4 induces acute beige adipogenesis and decreases adipogenic differentiation capacity long term. Overall, these findings indicate that the neonatal period is critical for adipocyte development and may influence the later onset of obesity.

scholarly journals Transforming Growth Factor-β3 Regulates Adipocyte Number in Subcutaneous White Adipose Tissue

Cell Reports ◽  
2018 ◽  
Vol 25 (3) ◽  
pp. 551-560.e5 ◽  
Author(s):  
Paul Petrus ◽  
Niklas Mejhert ◽  
Patricia Corrales ◽  
Simon Lecoutre ◽  
Qian Li ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Growth Factor ◽  
White Adipose Tissue ◽  
Transforming Growth Factor ◽  
Subcutaneous White Adipose Tissue

Abdominal Subcutaneous White Adipose Tissue in Surgically Slimmed Ex-Obese Shows the Same Molecular Features as Morbidly Obese Adipose Tissue

2011 ◽  
pp. OR11-4-OR11-4
Author(s):  
Raffaella Cancello ◽  
Alessandra Zulian ◽  
Davide Gentilini ◽  
Margherita Maffei ◽  
Alberto Della Barba ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
White Adipose Tissue ◽  
Morbidly Obese ◽  
Subcutaneous White Adipose Tissue ◽  
Molecular Features
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