Field Presence of Glyphosate-Resistant Horseweed (Conyza Canadensis), Common Lambsquarters (Chenopodium Album), and Giant Ragweed (Ambrosia Trifida) Biotypes with Elevated Tolerance to Glyphosate

2008 ◽  
Vol 22 (3) ◽  
pp. 544-548 ◽  
Author(s):  
Andrew M. Westhoven ◽  
Vince M. Davis ◽  
Kevin D. Gibson ◽  
Stephen C. Weller ◽  
William G. Johnson

Late-season field surveys conducted in Indiana from 2003 to 2005 showed that common lambsquarters and giant ragweed plants were present in 11 and 22%, respectively, of randomly sampled soybean fields that also contained glyphosate-resistant horseweed. In the fall of 2005 and 2006, seed from 13 common lambsquarters and 22 giant ragweed populations were collected from previously surveyed fields that had confirmed glyphosate-sensitive or -resistant horseweed. The objective of this study was to determine whether the presence of glyphosate-resistant horseweed was correlated with the presence of common lambsquarters and giant ragweed biotypes with elevated tolerance to glyphosate. Through a series of greenhouse screens, 57% of common lambsquarters and 31% of giant ragweed populations collected from fields that had glyphosate-resistant horseweed expressed elevated levels of glyphosate tolerance. However, elevated tolerance to glyphosate was expressed by 33% of giant ragweed and 100% of common lambsquarters populations collected in fields that had glyphosate-sensitive horseweed. Therefore, under the parameters of this experiment and through different types of analyses, we concluded there was not a strong correlation between the late-season presence of glyphosate-resistant horseweed and common lambsquarters and giant ragweed populations with elevated glyphosate tolerance in the same field. A number of the weed populations expressed significant stunting from exposure to glyphosate, but were able to resume growth. Thus, researchers should evaluate plant regrowth in addition to biomass suppression when making assessments of glyphosate resistance in weed populations through greenhouse and field screening.

2008 ◽  
Vol 22 (4) ◽  
pp. 628-634 ◽  
Author(s):  
Andrew M. Westhoven ◽  
Jeff M. Stachler ◽  
Mark M. Loux ◽  
William G. Johnson

Field studies were conducted in 2006 and 2007 to evaluate strategies for management of four glyphosate-tolerant common lambsquarters populations in glyphosate-resistant soybeans. Treatments consisted of several different preplant herbicide combinations followed by one or two postemergence applications of 0.84 to 3.36 kg ae/ha of glyphosate. Preplant application of a combination of glyphosate, 2,4-D ester and residual herbicides resulted in the most effective control of all populations, and allowed use of a single postemergence glyphosate application at the lowest rate. Late-season control of common lambsquarters ranged from 66 to 95% where no preplant herbicides were applied, 72 to 97% for preplant application of glyphosate and 2,4-D, and 96 to 100% for the combination of glyphosate, 2,4-D, flumioxazin, and cloransulam-methyl. Individual plants survived and produced seed following single postemergence glyphosate applications of 3.36 kg ae/ha, and multiple glyphosate applications totaling 2.5 kg ae/ha. Multiple postemergence treatments were more effective than single postemergence treatments for reduction of common lambsquarters population density and seed production. The progeny of plants that survived high rates of glyphosate in the field were screened for their response to glyphosate in the greenhouse. Progeny from one of three populations exhibited increased glyphosate tolerance from 1 yr of selection pressure.


Weed Science ◽  
2013 ◽  
Vol 61 (2) ◽  
pp. 289-295 ◽  
Author(s):  
Jessica R. Schafer ◽  
Steven G. Hallett ◽  
William G. Johnson

Root colonization by soil microorganisms has been shown to increase the activity of glyphosate in resistant and susceptible biotypes of giant ragweed and a susceptible common lambsquarters biotype, but not in horseweed biotypes. The objective of this study was to investigate the colonization of roots in glyphosate-resistant and -susceptible giant ragweed and horseweed biotypes, and glyphosate-tolerant and -susceptible biotypes of common lambsquarters after a sublethal glyphosate application. The three weed species were grown separately in sterile and unsterile field soil and treated with glyphosate at two sublethal rates. Soil microbes were isolated from the roots onto sterile media 3 d after the glyphosate treatment. The susceptible biotypes of giant ragweed and horseweed grown in unsterile soil were colonized by more soil microbes at the higher rate of glyphosate, compared to the resistant biotype grown in unsterile soil. Oomycetes were isolated separately on a selective media and they were also more prevalent in the roots of the susceptible biotypes of each weed species grown in the unsterile soil when glyphosate was applied at the highest rate. Therefore, the ability of these three weed species to tolerate a glyphosate application may involve differences in the susceptibility to soil microbial colonization, especially oomycetes.


2020 ◽  
Vol 34 (4) ◽  
pp. 607-612 ◽  
Author(s):  
Jessica Quinn ◽  
Nader Soltani ◽  
Jamshid Ashigh ◽  
David C. Hooker ◽  
Darren E. Robinson ◽  
...  

AbstractHorseweed is a competitive summer or winter annual weed that produces up to 230,000 small seeds per plant that are capable of traveling more than 500 km via wind. Giant ragweed is a tall, highly competitive summer annual weed. Glyphosate-resistant (GR) horseweed and GR giant ragweed pose significant challenges for producers in the United States and Ontario, Canada. It is thought that an integrated weed management (IWM) system involving herbicide rotation is required to control GR biotypes. Halauxifen-methyl is a new selective broadleaf POST herbicide registered for use in cereal crops; there is limited information on its efficacy on horseweed and giant ragweed. The purpose of this research was to determine the efficacy of halauxifen-methyl applied POST, alone and in a tank mix, for the control of GR horseweed and GR giant ragweed in wheat across southwestern Ontario. For each weed species, an efficacy study consisting of six field experiments was conducted over a 2-yr period (2018, 2019). At 8 wk after application (WAA), halauxifen-methyl, fluroxypyr/halauxifen-methyl, fluroxypyr/halauxifen-methyl + MCPA EHE, fluroxypyr + MCPA ester, 2,4-D ester, clopyralid, and pyrasulfotole/bromoxynil + ammonium sulfate controlled GR horseweed >95%. Fluroxypyr and MCPA provided only 86% and 37% control of GR horseweed, respectively. At 8 WAA, fluroxypyr, fluroxypyr/halauxifen-methyl, fluroxypyr/halauxifen-methyl + MCPA EHE, fluroxypyr + MCPA ester, fluroxypyr/halauxifen-methyl + MCPA EHE + pyroxsulam, 2,4-D ester, clopyralid, and thifensulfuron/tribenuron + fluroxypyr + MCPA ester controlled GR giant ragweed 87%, 88%, 90%, 94%, 96%, 96%, 98%, and 93%, respectively. Halauxifen-methyl and pyroxsulam provided only 45% and 28% control of GR giant ragweed, respectively. Halauxifen-methyl applied alone POST in the spring controlled GR horseweed but not GR giant ragweed in winter wheat.


2012 ◽  
Vol 26 (4) ◽  
pp. 657-660 ◽  
Author(s):  
Andrew P. Robinson ◽  
David M. Simpson ◽  
William G. Johnson

The introduction of 2,4-D-resistant crops stacked with glyphosate resistance will enable the use of 2,4-D and glyphosate for weed control in corn, cotton, and soybean. Because there is little reported on the effectiveness of 2,4-D plus glyphosate on summer annual weed control, the objective was to evaluate 2,4-D and glyphosate tank mixtures on summer annual weed control. Six rates of 2,4-D (0, 280, 420, 560, 840, and 1,120 g ae ha−1) and three rates of glyphosate (0, 840, and 1,120 g ae ha−1) were applied to common lambsquarters, common waterhemp, giant ragweed, giant foxtail, and velvetleaf. Glyphosate at 840 g ha−1controlled all weeds 94 to 100%. Giant ragweed was controlled 99 to 100% by 2,4-D alone when rates were 280 g ha−1or higher. Common lambsquarters, common waterhemp, and velvetleaf control increased as 2,4-D rates increased, with 1,120 g ha−1providing 90 to 94% control.


2007 ◽  
Vol 21 (1) ◽  
pp. 241-248 ◽  
Author(s):  
Dana B. Harder ◽  
Christy L. Sprague ◽  
Christina D. Difonzo ◽  
Karen A. Renner ◽  
Eric J. Ott ◽  
...  

Control of common lambsquarters with glyphosate in Michigan soybean fields has been inconsistent. Stem-boring insects and evidence of insect tunneling were found inside the stems of common lambsquarters plants not controlled with glyphosate. In 2004 and 2005, field surveys and studies were conducted to identify and evaluate the prevalence of stem-boring insects in common lambsquarters in Michigan and Indiana soybean fields to determine whether tunneling by insects occurred before or following POST glyphosate applications and to evaluate the effect of glyphosate rate, application timing, and insect tunneling on the control of common lambsquarters with glyphosate. Two insect species, the beet petiole borer (Cosmobaris americana) from the Curculionidae family and an unidentified leafminer fly larvae from the Agromyzidae family were found inside common lambsquarters stems. Leafminer larvae were present in Michigan soybean fields in mid- to late-June, when most POST glyphosate applications are made in Michigan and Indiana; however, beet petiole borer larvae were not found in common lambsquarters stems until mid-July and would only be present in common lambsquarters plants if glyphosate applications occurred at that time. Results from three field experiments in East Lansing, MI, demonstrated the variability in common lambsquarters control. Control ranged from 79 to 98%, 75 to 99%, and 49 to 97% from glyphosate applied at 0.84 kgae/ha to 10-, 25-, and 46-cm common lambsquarters, respectively. In general, applying glyphosate to common lambsquarters plants 10 cm or less, or increasing the glyphosate rate beyond 0.84 kgae/ha, improved common lambsquarters control. Insect tunneling by leafminer and beet petiole borer larvae did not contribute to reduced common lambsquarters control with glyphosate applied to 10- and 25-cm common lambsquarters.


Weed Science ◽  
2011 ◽  
Vol 59 (4) ◽  
pp. 506-511 ◽  
Author(s):  
Chad B. Brabham ◽  
Corey K. Gerber ◽  
William G. Johnson

Glyphosate-resistant giant ragweed has become an increasing problem, and the potential spread of these biotypes is a threat to production agriculture and to the long-term utility of glyphosate and glyphosate-resistant crops. The fate of glyphosate resistance in a giant ragweed population is dependent on the fitness of the resistant biotype. Our objective was to determine the fitness of glyphosate-resistant giant ragweed in the absence and presence of glyphosate. We compared the growth and seed production of glyphosate-resistant (GR) and glyphosate-susceptible (GS) giant ragweed under field conditions in the absence of glyphosate. A greenhouse study was also conducted to determine the survival of GR and GS giant ragweed and their open-pollinated progeny from the field study under glyphosate-induced selection pressure. In the absence of glyphosate, GR giant ragweed displayed rapid, early season growth, but 50 d after planting, the biotypes were similar in height, shoot weight, and leaf area. During reproduction, the GR biotype flowered earlier and produced 25% less seed than the GS biotype. In the presence of glyphosate, an outcrossing rate of 31% was detected between GR and GS biotypes because 61% of progeny were resistant to glyphosate at 840 g ae ha−1. A second application 14 d later at 2,520 g ae ha−1completely removed the GS alleles from the population, whereas several homozygous and heterozygous GR plants survived and produced seed. These results indicate GR will persist in the population when subjected to glyphosate and that glyphosate resistance in giant ragweed has the potential to spread rapidly in our current agricultural ecosystem.


Weed Science ◽  
2017 ◽  
Vol 65 (5) ◽  
pp. 588-602 ◽  
Author(s):  
Zahoor A. Ganie ◽  
Mithila Jugulam ◽  
Amit J. Jhala

Glyphosate and 2,4-D have been commonly used for control of common and giant ragweed before planting of corn and soybean in the midwestern United States. Because these herbicides are primarily applied in early spring, environmental factors such as temperature may influence their efficacy. The objectives of this study were to (1) evaluate the influence of temperature on the efficacy of 2,4-D or glyphosate for common and giant ragweed control and the level of glyphosate resistance and (2) determine the underlying physiological mechanisms (absorption and translocation). Glyphosate-susceptible (GS) and glyphosate-resistant (GR) common and giant ragweed biotypes from Nebraska were used for glyphosate dose–response studies, and GR biotypes were used for 2,4-D dose–response studies conducted at two temperatures (day/night [d/n]; low temperature [LT]: 20/11 C d/n; high temperature [HT]: 29/17 C d/n). Results indicate improved efficacy of 2,4-D or glyphosate at HT compared with LT for common and giant ragweed control regardless of susceptibility or resistance to glyphosate. The level of glyphosate resistance decreased in both the species at HT compared with LT, primarily due to more translocation at HT. More translocation of 2,4-D in GR common and giant ragweed at HT compared with LT at 96 h after treatment could be the reason for improved efficacy. Similarly, higher translocation in common ragweed and increased absorption and translocation in giant ragweed resulted in greater efficacy of glyphosate at HT compared with LT. It is concluded that the efficacy of 2,4-D or glyphosate for common and giant ragweed control can be improved if applied at warm temperatures (29/17 C d/n) due to increased absorption and/or translocation compared with applications during cooler temperatures (20/11 C d/n).


2011 ◽  
Vol 25 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Mark M. Loux ◽  
Anthony F. Dobbels ◽  
William G. Johnson ◽  
Bryan G. Young

Field studies were conducted in 2007 and 2008 at seven sites in Ohio, Indiana, and Illinois to determine the effect of PRE herbicide and POST application timing on weed control and yield of glyphosate-resistant corn. Levels of PRE herbicide included none; low—atrazine; medium—atrazine and metolachlor; and high—atrazine, mesotrione, and metolachlor. Glyphosate was applied POST when corn was 30 cm tall, or 1 or 2 wk later. Common lambsquarters, giant foxtail, and giant ragweed infested at least six of the seven sites, and other weed species occurred at two to three sites. Control of weeds at the time of POST application ranged from 48 to 91%, 58 to 99%, and 87 to 100% for the low, medium, and high levels of PRE herbicide, respectively, averaged over POST application timing. Control of giant foxtail and redroot pigweed decreased by about 20% between the second and third POST timing, averaged over PRE herbicide, but control of other weeds was similar among timings. Late-season control of common ragweed, velvetleaf, common lambsquarters, and Pennsylvania smartweed exceeded 90%, regardless of PRE herbicide or POST timing. Control of redroot pigweed, ivyleaf morningglory, and giant ragweed was as low as 74, 67, and 83%, respectively, but the high level of PRE herbicide resulted in 90 to 97% control of these weeds. An interaction between PRE herbicide and POST timing for late-season control of giant foxtail, tall waterhemp, and yellow nutsedge reflected the more effective control among POST timings from the higher levels of PRE herbicide. The overall trend in this study was for more effective weed control in PRE/POST herbicide programs with more comprehensive PRE herbicides that have substantial activity on both grass and broadleaf weeds. Highest yield occurred where the PRE treatment consisted of a two- or three-way combination of herbicides applied at 50% of the recommended rate or higher. Yield was reduced at all POST timings with atrazine alone or in the absence of PRE herbicide.


2015 ◽  
Vol 06 (13) ◽  
pp. 2104-2113 ◽  
Author(s):  
Vijay K. Nandula ◽  
Alice A. Wright ◽  
Christopher R. Van Horn ◽  
William T. Molin ◽  
Phil Westra ◽  
...  

2011 ◽  
Vol 25 (3) ◽  
pp. 430-435 ◽  
Author(s):  
Jason K. Norsworthy ◽  
Dilpreet Riar ◽  
Prashant Jha ◽  
Robert C. Scott

Glyphosate-resistant giant ragweed in Arkansas was reported in 2005. A study was conducted to (1) confirm and characterize the glyphosate resistance in giant ragweed, (2) determine if reduced absorption or translocation is the mechanism of glyphosate resistance in giant ragweed, and (3) evaluate the efficacy of nine POST-applied soybean herbicides to control glyphosate-resistant and -susceptible giant ragweed. Based on the rate required to kill 50% of plants (LD50 values), resistant giant ragweed biotypes from Greene and Jefferson counties were 2.3- to 7.2-fold less sensitive to glyphosate compared to susceptible biotypes. Glyphosate absorption and translocation for glyphosate-resistant and -susceptible biotypes was similar at 24 and 72 h after treatment. Thus, differential absorption or translocation is not a mechanism of glyphosate resistance in this resistant giant ragweed biotype. Control of resistant giant ragweed biotypes with glyphosate at a labeled field application rate of 840 g ha−1 was only 60% or less compared to complete control of a susceptible giant ragweed biotype. However, bentazon, carfentrazone, cloransulam, and fomesafen controlled both biotypes more than 95%.


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