Expression Patterns of Three Heat Shock Proteins in Chilo suppressalis (Lepidoptera: Pyralidae)

2014 ◽  
Vol 107 (3) ◽  
pp. 667-673 ◽  
Author(s):  
Ming-Xing Lu ◽  
Zhong-Xian Liu ◽  
Ya-Dong Cui ◽  
Yu-Zhou Du
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Expression Patterns ◽  
Chilo Suppressalis ◽  
Lepidoptera Pyralidae ◽  
Shock Proteins

scholarly journals Synergistic Effects of Toxic Elements on Heat Shock Proteins

2014 ◽  
Vol 2014 ◽  
pp. 1-17 ◽  
Author(s):  
Khalid Mahmood ◽  
Saima Jadoon ◽  
Qaisar Mahmood ◽  
Muhammad Irshad ◽  
Jamshaid Hussain
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Toxic Elements ◽  
Current Knowledge ◽  
Synergistic Effects ◽  
Expression Patterns ◽  
Expression Levels ◽  
Functional Capabilities ◽  
Shock Proteins ◽  
Diverse Groups

Heat shock proteins show remarkable variations in their expression levels under a variety of toxic conditions. A research span expanded over five decades has revealed their molecular characterization, gene regulation, expression patterns, vast similarity in diverse groups, and broad range of functional capabilities. Their functions include protection and tolerance against cytotoxic conditions through their molecular chaperoning activity, maintaining cytoskeleton stability, and assisting in cell signaling. However, their role as biomarkers for monitoring the environmental risk assessment is controversial due to a number of conflicting, validating, and nonvalidating reports. The current knowledge regarding the interpretation of HSPs expression levels has been discussed in the present review. The candidature of heat shock proteins as biomarkers of toxicity is thus far unreliable due to synergistic effects of toxicants and other environmental factors. The adoption of heat shock proteins as “suit of biomarkers in a set of organisms” requires further investigation.

scholarly journals Transcriptional Program of Early Sporulation and Stationary-Phase Events in Clostridium acetobutylicum

2005 ◽  
Vol 187 (20) ◽  
pp. 7103-7118 ◽  
Author(s):  
Keith V. Alsaker ◽  
Eleftherios T. Papoutsakis
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Stationary Phase ◽  
Clostridium Acetobutylicum ◽  
Expression Patterns ◽  
Primary Metabolism ◽  
Amino Acid Synthesis ◽  
Shock Proteins ◽  
Sporulation Genes

ABSTRACT DNA microarray analysis of Clostridium acetobutylicum was used to examine the genomic-scale gene expression changes during the shift from exponential-phase growth and acidogenesis to stationary phase and solventogenesis. Self-organizing maps were used to identify novel expression patterns of functional gene classes, including aromatic and branched-chain amino acid synthesis, ribosomal proteins, cobalt and iron transporters, cobalamin biosynthesis, and lipid biosynthesis. The majority of pSOL1 megaplasmid genes (in addition to the solventogenic genes aad-ctfA-ctfB and adc) had increased expression at the onset of solventogenesis, suggesting that other megaplasmid genes may play a role in stationary-phase phenomena. Analysis of sporulation genes and comparison with published Bacillus subtilis results indicated conserved expression patterns of early sporulation genes, including spo0A, the sigF operon, and putative canonical genes of the σH and σF regulons. However, sigE expression could not be detected within 7.5 h of initial spo0A expression, consistent with the observed extended time between the appearance of clostridial forms and endospore formation. The results were compared with microarray comparisons of the wild-type strain and the nonsolventogenic, asporogenous M5 strain, which lacks the pSOL1 megaplasmid. While some results were similar, the expression of primary metabolism genes and heat shock proteins was higher in M5, suggesting a difference in metabolic regulation or a butyrate stress response in M5. The results of this microarray platform and analysis were further validated by comparing gene expression patterns to previously published Northern analyses, reporter assays, and two-dimensional protein electrophoresis data of metabolic genes (including all major solventogenesis genes), sporulation genes, heat shock proteins, and other solventogenesis-induced gene expression.

Visualization of Heat Shock Proteins for Quantifying Laser-Induced Thermal Ablation of Biological Tissues

2011 ◽  
Author(s):  
Amir Y. Sajjadi ◽  
Kunal Mitra ◽  
Michael S. Grace
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Laser Irradiation ◽  
Expression Patterns ◽  
Biological Tissues ◽  
Threshold Temperature ◽  
Target Tissue ◽  
Laser Induced Damage ◽  
Shock Proteins ◽  
Over Time

In laser-based therapeutics, it is important to ablate target tissue with minimal damage to surrounding healthy tissue. Unique properties of lasers allow precise and controlled ablation of tissue. Tightly focusing a short-pulse laser at the desired tissue region and controlling the exposure time by scanning the beam at the target can minimize corresponding collateral damage [1]. Even so, design of effective laser-based ablation procedures requires an understanding of the extent of laser-induced damage for given laser parameters (power, intensity, duration, etc.). Therefore, the instantaneous and effects over time of laser irradiation in live tissue should be studied. Instantaneous effects can be quantified by measuring thermal effects of laser irradiation on tissue. Depending on the application, threshold temperature is necessary to make permanent or temporary changes in tissue structure [1]. The temperature profile around the laser-irradiated region gives insight into radial energy spread and the extent of damage in tissue surrounding the ablation zone. In order to investigate the effects over time of laser irradiation of tissue, we studied the temporal expression patterns heat shock proteins (HSP), members of a class of proteins whose expression patterns change when cells are exposed to elevated temperature or other stressors [2]. We conducted experiments on live anesthetized mice to determine the spatiotemporal expression patterns of heat shock proteins in skin tissue after laser stimulation, both to understand the roles of heat shock proteins in laser-induced tissue damage and repair, and to develop heat shock proteins as tools to illustrate the extent of laser-induced damage and wound healing following irradiation.

scholarly journals 607 Modulation of Heat Shock Proteins in Potato Leaves by Rhizospheric Calcium: Mitigation of Heat Stress Effect

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 501E-502
Author(s):  
Sookhee Park ◽  
Jiwan P. Palta
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Calcium Concentration ◽  
Expression Patterns ◽  
Dry Weight ◽  
Potato Production ◽  
Stress Effect ◽  
Shock Proteins

High temperature effects potato production by reducing overall growth and partitioning of photosynthate to tubers. Recent studies from our laboratory demonstrated that these effects can be reduced by increasing rhizospheric calcium. This present study was conducted to determine if this mitigation of heat stress effect on potato is due to modulation of heat shock protein by calcium during stress. An inert medium and nutrient delivery system capable of maintaining precise rhizospheric calcium levels were used. Biomass was measured and protein samples were collected from potato leaves. Using electroblotting, heat shock proteins were detected by antibodies to Hsp21 and Hsp70 (obtained from Dr. Elizabeth Vierling). Injury by prolonged heat stress was mitigated at calcium concentration >5 ppm. The calcium concentration of leaf and stem tissues were twice as high in 25 ppm calcium-treated plant compared to 1 ppm calcium-treated plants. Total foliage fresh weight was 33% higher and dry weight 20% higher in plants supplied with 25 ppm of calcium than supplied with 1 ppm of calcium. HSP21 was expressed only at high temperature and at greater concentrations in 25 ppm calcium treatment. HSP70 was expressed in both control, 20 °C/15 °C (day/night) and heat-stressed tissue, 35 °C/25 °C (day/night) under various calcium treatments (1 to 25 ppm). Also, there were some differences in HSPs expression patterns between young and mature leaves. Young tissue responded immediately to the heat stress and started to express HSP21 within 1 day. Mature tissue started to express HSP21 after 2 days. HSP21 of young tissue disappeared sooner than mature tissue when heat stress-treated plants were returned to normal conditions. These results support our earlier studies indicating that an increase in rhizospheric calcium mitigate heat stress effects on the potato plant. Furthermore these results suggest that this mitigation may be due to modulation of HSP21by rhizospheric calcium during heat stress.

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