Visualization of Heat Shock Proteins for Quantifying Laser-Induced Thermal Ablation of Biological Tissues

2011 ◽  
Author(s):  
Amir Y. Sajjadi ◽  
Kunal Mitra ◽  
Michael S. Grace
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Laser Irradiation ◽  
Expression Patterns ◽  
Biological Tissues ◽  
Threshold Temperature ◽  
Target Tissue ◽  
Laser Induced Damage ◽  
Shock Proteins ◽  
Over Time

In laser-based therapeutics, it is important to ablate target tissue with minimal damage to surrounding healthy tissue. Unique properties of lasers allow precise and controlled ablation of tissue. Tightly focusing a short-pulse laser at the desired tissue region and controlling the exposure time by scanning the beam at the target can minimize corresponding collateral damage [1]. Even so, design of effective laser-based ablation procedures requires an understanding of the extent of laser-induced damage for given laser parameters (power, intensity, duration, etc.). Therefore, the instantaneous and effects over time of laser irradiation in live tissue should be studied. Instantaneous effects can be quantified by measuring thermal effects of laser irradiation on tissue. Depending on the application, threshold temperature is necessary to make permanent or temporary changes in tissue structure [1]. The temperature profile around the laser-irradiated region gives insight into radial energy spread and the extent of damage in tissue surrounding the ablation zone. In order to investigate the effects over time of laser irradiation of tissue, we studied the temporal expression patterns heat shock proteins (HSP), members of a class of proteins whose expression patterns change when cells are exposed to elevated temperature or other stressors [2]. We conducted experiments on live anesthetized mice to determine the spatiotemporal expression patterns of heat shock proteins in skin tissue after laser stimulation, both to understand the roles of heat shock proteins in laser-induced tissue damage and repair, and to develop heat shock proteins as tools to illustrate the extent of laser-induced damage and wound healing following irradiation.

Short-term exercise training can improve myocardial tolerance to I/R without elevation in heat shock proteins

2001 ◽  
Vol 281 (3) ◽  
pp. H1346-H1352 ◽  
Author(s):  
Karyn L. Hamilton ◽  
Scott K. Powers ◽  
Takao Sugiura ◽  
Sunjoo Kim ◽  
Shannon Lennon ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Ischemia Reperfusion ◽  
Left Ventricular ◽  
Antioxidant Defenses ◽  
Control Group ◽  
Shock Proteins ◽  
Mnsod Activity ◽  
Over Time

We examined the effects of 3 days of exercise in a cold environment on the expression of left ventricular (LV) heat shock proteins (HSPs) and contractile performance during in vivo ischemia-reperfusion (I/R). Sprague-Dawley rats were divided into the following three groups ( n = 12/group): 1) control, 2) exercise (60 min/day) at 4°C (E-Cold), and 3) exercise (60 min/day) at 25°C (E-Warm). Left anterior descending coronary occlusion was maintained for 20 min, followed by 30 min of reperfusion. Compared with the control group, both the E-Cold and E-Warm groups maintained higher ( P < 0.05) LV developed pressure, first derivative of pressure development over time (+dP/d t), and pressure relaxation over time (−dP/d t) throughout I/R. Relative levels of HSP90, HSP72, and HSP40 were higher ( P < 0.05) in E-Warm animals compared with both control and E-Cold. HSP10, HSP60, and HSP73 did not differ between groups. Exercise increased manganese superoxide dismutase (MnSOD) activity in both E-Warm and E-Cold hearts ( P < 0.05). Protection against I/R-induced lipid peroxidation in the LV paralleled the increase in MnSOD activity whereas lower levels of lipid peroxidation were observed in both E-Warm and E-Cold groups compared with control. We conclude that exercise-induced myocardial protection against a moderate duration I/R insult is not dependent on increases in myocardial HSPs. We postulate that exercise-associated cardioprotection may depend, in part, on increases in myocardial antioxidant defenses.

Heat shock protein synthesis over time in infective Trichinella spiralis larvae raised in suboptimal culture conditions

2004 ◽  
Vol 78 (3) ◽  
pp. 243-247 ◽  
Author(s):  
J. Martinez ◽  
J. Perez-Serrano ◽  
W.E. Bernadina ◽  
I. Rincon ◽  
F. Rodriguez-Caabeiro
Keyword(s):  
Cell Death ◽  
Protein Synthesis ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Heat Shock Protein ◽  
Trichinella Spiralis ◽  
Culture Conditions ◽  
Induce Stress ◽  
Shock Proteins ◽  
Over Time

AbstractChanges in the viability, infectivity and heat shock protein (Hsp) levels are reported in Trichinella spiralis first stage larvae (L1) stored in 199 medium for up to seven days at 37°C. These conditions induce stress that the larvae, eventually, cannot overcome. After three days of storage, the infectivity and viability were unchanged, although higher Hsp70 levels were observed. After this time, larvae gradually lost viability and infectivity, coinciding with a decrease in Hsp70 and Hsp90 and an increase in actin (a housekeeping protein). In addition, a possibly inducible heat shock protein, Hsp90i, appeared as constitutive Hsp90 disappeared. No significant changes in Hsp60 levels were detected at any time. These results suggest that heat shock proteins initially try to maintain homeostasis, but on failing, may be involved in cell death.

scholarly journals Synergistic Effects of Toxic Elements on Heat Shock Proteins

2014 ◽  
Vol 2014 ◽  
pp. 1-17 ◽  
Author(s):  
Khalid Mahmood ◽  
Saima Jadoon ◽  
Qaisar Mahmood ◽  
Muhammad Irshad ◽  
Jamshaid Hussain
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Toxic Elements ◽  
Current Knowledge ◽  
Synergistic Effects ◽  
Expression Patterns ◽  
Expression Levels ◽  
Functional Capabilities ◽  
Shock Proteins ◽  
Diverse Groups

Heat shock proteins show remarkable variations in their expression levels under a variety of toxic conditions. A research span expanded over five decades has revealed their molecular characterization, gene regulation, expression patterns, vast similarity in diverse groups, and broad range of functional capabilities. Their functions include protection and tolerance against cytotoxic conditions through their molecular chaperoning activity, maintaining cytoskeleton stability, and assisting in cell signaling. However, their role as biomarkers for monitoring the environmental risk assessment is controversial due to a number of conflicting, validating, and nonvalidating reports. The current knowledge regarding the interpretation of HSPs expression levels has been discussed in the present review. The candidature of heat shock proteins as biomarkers of toxicity is thus far unreliable due to synergistic effects of toxicants and other environmental factors. The adoption of heat shock proteins as “suit of biomarkers in a set of organisms” requires further investigation.

scholarly journals Transcriptional Program of Early Sporulation and Stationary-Phase Events in Clostridium acetobutylicum

2005 ◽  
Vol 187 (20) ◽  
pp. 7103-7118 ◽  
Author(s):  
Keith V. Alsaker ◽  
Eleftherios T. Papoutsakis
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Stationary Phase ◽  
Clostridium Acetobutylicum ◽  
Expression Patterns ◽  
Primary Metabolism ◽  
Amino Acid Synthesis ◽  
Shock Proteins ◽  
Sporulation Genes

ABSTRACT DNA microarray analysis of Clostridium acetobutylicum was used to examine the genomic-scale gene expression changes during the shift from exponential-phase growth and acidogenesis to stationary phase and solventogenesis. Self-organizing maps were used to identify novel expression patterns of functional gene classes, including aromatic and branched-chain amino acid synthesis, ribosomal proteins, cobalt and iron transporters, cobalamin biosynthesis, and lipid biosynthesis. The majority of pSOL1 megaplasmid genes (in addition to the solventogenic genes aad-ctfA-ctfB and adc) had increased expression at the onset of solventogenesis, suggesting that other megaplasmid genes may play a role in stationary-phase phenomena. Analysis of sporulation genes and comparison with published Bacillus subtilis results indicated conserved expression patterns of early sporulation genes, including spo0A, the sigF operon, and putative canonical genes of the σH and σF regulons. However, sigE expression could not be detected within 7.5 h of initial spo0A expression, consistent with the observed extended time between the appearance of clostridial forms and endospore formation. The results were compared with microarray comparisons of the wild-type strain and the nonsolventogenic, asporogenous M5 strain, which lacks the pSOL1 megaplasmid. While some results were similar, the expression of primary metabolism genes and heat shock proteins was higher in M5, suggesting a difference in metabolic regulation or a butyrate stress response in M5. The results of this microarray platform and analysis were further validated by comparing gene expression patterns to previously published Northern analyses, reporter assays, and two-dimensional protein electrophoresis data of metabolic genes (including all major solventogenesis genes), sporulation genes, heat shock proteins, and other solventogenesis-induced gene expression.

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