Molecular Genetic Investigation of Morphological Species of Members of the Neomelaniconion Subgenus (Diptera: Culicidae: Aedini) From Madagascar Using Ribosomal Internal Transcribed Spacer 2

2009 ◽  
Vol 46 (2) ◽  
pp. 403-407 ◽  
Author(s):  
Pierre Kengne ◽  
Gilbert Le Goff ◽  
Didier Fontenille
Keyword(s):  
Internal Transcribed Spacer ◽  
Molecular Genetic ◽  
Genetic Investigation ◽  
Internal Transcribed Spacer 2 ◽  
Morphological Species

scholarly journals The new Internal Transcribed Spacer 2 diagnostic tool clarifies the taxonomic position and geographic distribution of the North American malaria vector Anopheles punctipennis

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
James M. Hodge ◽  
Andrey A. Yurchenko ◽  
Dmitriy A. Karagodin ◽  
Reem A. Masri ◽  
Ryan C. Smith ◽  
...  
Keyword(s):  
Malaria Vector ◽  
Internal Transcribed Spacer ◽  
Single Species ◽  
Its2 Sequence ◽  
Pathogen Transmission ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Sequence Length ◽  
Phylogenetic Position ◽  
Internal Transcribed Spacer 2

Abstract Background The malaria mosquito Anopheles punctipennis, a widely distributed species in North America, is capable of transmitting human malaria and is actively involved in the transmission of the ungulate malaria parasite Plasmodium odocoilei. However, molecular diagnostic tools based on Internal Transcribed Spacer 2 (ITS2) of ribosomal DNA are lacking for this species. Anopheles punctipennis is a former member of the Anopheles maculipennis complex but its systematic position remains unclear. Methods In this study, ITS2 sequences were obtained from 276 An. punctipennis specimens collected in the eastern and midwestern United States and a simple and robust Restriction Fragment Length Polymorphism approach for species identification was developed. The maximum-likelihood phylogenetic tree was constructed based on ITS2 sequences available through this study and from GenBank for 20 species of Anopheles. Results The analysis demonstrated a consistent ITS2 sequence length and showed no indications of intragenomic variation among the samples based on ITS2, suggesting that An. punctipennis represents a single species in the studied geographic locations. In this study, An. punctipennis was found in urban, rural, and forest settings, suggesting its potential broad role in pathogen transmission. Phylogeny based on ITS2 sequence comparison demonstrated the close relationship of this species with other members of the Maculipennis group. Conclusions This study developed molecular tools based on ITS2 sequences for the malaria vector An. punctipennis and clarified the phylogenetic position of the species within the Maculipennis group.

Clinical and genetic investigation of 136 Japanese patients with congenital hypothyroidism

2020 ◽  
Vol 33 (6) ◽  
pp. 691-701 ◽  
Author(s):  
Tatsushi Tanaka ◽  
Kohei Aoyama ◽  
Atsushi Suzuki ◽  
Shinji Saitoh ◽  
Haruo Mizuno
Keyword(s):  
Congenital Hypothyroidism ◽  
Molecular Genetic ◽  
Genetic Diagnosis ◽  
Japanese Patients ◽  
Thyroid Stimulating Hormone ◽  
Allelic Variant ◽  
Genetic Investigation ◽  
Pathogenic Variants ◽  
Recessive Genes ◽  
Molecular Genetic Diagnosis

AbstractObjectivesCongenital hypothyroidism (CH) is the most common congenital endocrine disorder. Recent advances in genetic testing have revealed its causative mutations in some CH patients. However, the underlying etiology remains unknown in most patients. This study aimed to perform clinical and genetic investigation in Japanese CH patients to uncover genotype-phenotype correlations.MethodsWe enrolled 136 Japanese patients with transient or permanent CH between April 2015 and March 2017, and performed next-generation sequencing of 19 genes implicated in CH.ResultsWe identified potentially pathogenic bi-allelic variants in DUOX2, TSHR, and TPO in 19, 5, and 1 patient, respectively (autosomal recessive), and a potentially pathogenic mono-allelic variant in NKX2-1 (autosomal dominant) in 1 patient. Molecular genetic diagnosis was highly suggested in 26 patients (19%) from 23 families. We also detected a potentially pathogenic mono-allelic variant in five recessive genes (DUOX2, TSHR, TG, DUOXA2, and TPO) in 31 unrelated patients (23%), although the pathogenicity of these variants remains inconclusive. Patients with bi-allelic DUOX2 variants showed a more severe clinical presentation in infancy than those with bi-allelic TSHR variants. However, this trend reversed beyond infancy. There were no statistical differences in initial thyroid stimulating hormone, free thyroxine, thyroglobulin, and levothyroxine dose as of March 2017 between patients with bi-allelic and mono-allelic DUOX2 variants.ConclusionsThe prevalence of potentially-pathogenic variants in Japanese CH patients was similar to that found by previous reports. Our study demonstrates a genotype-phenotype correlation in Japanese CH patients.

scholarly journals Rhizospheric Microbiome Profiling of Capsicum annuum L. Cultivated in Amended Soils by 16S and Internal Transcribed Spacer 2 rRNA Amplicon Metagenome Sequencing

2017 ◽  
Vol 5 (30) ◽  
Author(s):  
Ali Asaff-Torres ◽  
Mariana Armendáriz-Ruiz ◽  
Manuel Kirchmayr ◽  
Raúl Rodríguez-Heredia ◽  
Marcos Orozco ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Internal Transcribed Spacer ◽  
Root Exudate ◽  
Microbial Consortium ◽  
Amended Soils ◽  
Internal Transcribed Spacer 2 ◽  
Capsicum Annuum L ◽  
Metagenome Sequencing ◽  
Microbiome Profiling

ABSTRACT Rhizospheric microbiomes of Capsicum annuum L. cultivated either conventionally or amended with a synthetic microbial consortium or a root exudate inductor, were characterized by 16S/internal transcribed spacer 2 (ITS2) rRNA amplicon metagenome sequencing. The most abundant taxa found, although differently represented in each treatment, were Gammaproteobacteria, Alphaproteobacteria, Actinobacteria, and Bacilli, as well as Chytridiomycetes and Mortierellomycotina.

scholarly journals Internal transcribed spacer 2 (ITS2) barcodes: A useful tool for identifying ChineseZanthoxylum

2018 ◽  
Vol 6 (6) ◽  
pp. e01157 ◽  
Author(s):  
Li-Li Zhao ◽  
Shi-Jing Feng ◽  
Jie-Yun Tian ◽  
An-Zhi Wei ◽  
Tu-Xi Yang
Keyword(s):  
Internal Transcribed Spacer ◽  
Internal Transcribed Spacer 2

A Korean Patient with Early Juvenile Form of Metachromatic Leukodystrophy: Biochemical and Molecular Genetic Investigation

2017 ◽  
Vol 7 (1) ◽  
pp. 41
Author(s):  
Yeong-Bin Kim ◽  
Hyung-Doo Park ◽  
Rihwa Choi ◽  
Soo-Youn Lee ◽  
Chang-Seok Ki ◽  
...  
Keyword(s):  
Metachromatic Leukodystrophy ◽  
Molecular Genetic ◽  
Genetic Investigation ◽  
Juvenile Form ◽  
Early Juvenile ◽  
Korean Patient
Sign in / Sign up

Export Citation Format

Share Document