Nomenclature Abstract for Moorella thermoacetica (Fontaine et al. 1942) Collins et al. 1994.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
George M Garrity
Keyword(s):  
Moorella Thermoacetica

Exemplar Abstract for Moorella thermoacetica (Fontaine et al. 1942) Collins et al. 1994.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Moorella Thermoacetica

scholarly journals Metabolic engineering of Moorella thermoacetica for thermophilic bioconversion of gaseous substrates to a volatile chemical

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Junya Kato ◽  
Kaisei Takemura ◽  
Setsu Kato ◽  
Tatsuya Fujii ◽  
Keisuke Wada ◽  
...  
Keyword(s):  
Carbon Flux ◽  
Cost Effective ◽  
Gas Composition ◽  
Autotrophic Growth ◽  
Acetyl Coenzyme A ◽  
Volatile Chemicals ◽  
Moorella Thermoacetica ◽  
Gas Fermentation ◽  
Dry Cell ◽  
Co Gas

AbstractGas fermentation is one of the promising bioprocesses to convert CO2 or syngas to important chemicals. Thermophilic gas fermentation of volatile chemicals has the potential for the development of consolidated bioprocesses that can simultaneously separate products during fermentation. This study reports the production of acetone from CO2 and H2, CO, or syngas by introducing the acetone production pathway using acetyl–coenzyme A (Ac-CoA) and acetate produced via the Wood–Ljungdahl pathway in Moorella thermoacetica. Reducing the carbon flux from Ac-CoA to acetate through genetic engineering successfully enhanced acetone productivity, which varied on the basis of the gas composition. The highest acetone productivity was obtained with CO–H2, while autotrophic growth collapsed with CO2–H2. By adding H2 to CO, the acetone productivity from the same amount of carbon source increased compared to CO gas only, and the maximum specific acetone production rate also increased from 0.04 to 0.09 g-acetone/g-dry cell/h. Our development of the engineered thermophilic acetogen M. thermoacetica, which grows at a temperature higher than the boiling point of acetone (58 °C), would pave the way for developing a consolidated process with simplified and cost-effective recovery via condensation following gas fermentation.

scholarly journals Efficient Production of Acetic Acid from Nipa (Nypa fruticans) Sap by Moorella thermoacetica (f. Clostridium thermoaceticum)

2019 ◽  
Author(s):  
Dung Van Nguyen ◽  
Pinthep Sethapokin ◽  
Harifara Rabemanolontsoa ◽  
Eiji Minami ◽  
Haruo Kawamoto ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Oxalic Acid ◽  
Acid Catalyst ◽  
Cost Effective ◽  
Efficient Production ◽  
Acid Fermentation ◽  
Acid Yield ◽  
Moorella Thermoacetica ◽  
Nypa Fruticans ◽  
Hydrolysis Of

To valorize the underutilized nipa sap composed mainly of sucrose, glucose and fructose, acetic acid fermentation by Moorella thermoacetica was explored. Given that M. thermoacetica cannot directly metabolize sucrose, we evaluated various catalysts for the hydrolysis of this material. Oxalic acid and invertase exhibited high levels of activity towards the hydrolysis of the sucrose in nipa sap to glucose and fructose. Although these two methods consumed similar levels of energy for the hydrolysis of sucrose, oxalic acid was found to be more cost-effective. Nipa saps hydrolyzed by these two catalysts were also fermented by M. thermoacetica. The results revealed that the two hydrolyzed sap mixtures gave 10.0 g/L of acetic acid from the 10.2 g/L of substrate sugars in nipa sap. Notably, the results showed that the oxalic acid catalyst was also fermented to acetic acid, which avoided the need to remove the catalyst from the product stream. Taken together, these results show that oxalic acid hydrolysis is superior to enzymatic hydrolysis for the pretreatment of nipa sap. The acetic acid yield achieved in this study corresponds to a conversion efficiency of 98%, which is about 3.6 times higher than that achieved using the traditional methods. The process developed in this study therefore has high potential as a green biorefinery process for the efficient conversion of sucrose-containing nipa sap to bio-derived acetic acid.

Investigating Moorella thermoacetica metabolism with a genome-scale constraint-based metabolic model

2015 ◽  
Vol 7 (8) ◽  
pp. 869-882 ◽  
Author(s):  
M. Ahsanul Islam ◽  
Karsten Zengler ◽  
Elizabeth A. Edwards ◽  
Radhakrishnan Mahadevan ◽  
Gregory Stephanopoulos
Keyword(s):  
Metabolic Model ◽  
Strictly Anaerobic ◽  
Moorella Thermoacetica ◽  
A Genome ◽  
Acetogenic Bacterium ◽  
Genome Scale

Moorella thermoaceticais a strictly anaerobic, endospore-forming, and metabolically versatile acetogenic bacterium capable of conserving energy by both autotrophic (acetogenesis) and heterotrophic (homoacetogenesis) modes of metabolism.

Influence of nitrate on oxalate- and glyoxylate-dependent growth and acetogenesis by Moorella thermoacetica

2002 ◽  
Vol 178 (6) ◽  
pp. 457-464 ◽  
Author(s):  
Corinna Seifritz ◽  
Jürgen Fröstl ◽  
Harold Drake ◽  
Steven Daniel
Keyword(s):  
Moorella Thermoacetica ◽  
Dependent Growth

scholarly journals Binding site for coenzyme A revealed in the structure of pyruvate:ferredoxin oxidoreductase from Moorella thermoacetica

2018 ◽  
Vol 115 (15) ◽  
pp. 3846-3851 ◽  
Author(s):  
Percival Yang-Ting Chen ◽  
Heather Aman ◽  
Mehmet Can ◽  
Stephen W. Ragsdale ◽  
Catherine L. Drennan
Keyword(s):  
Carbon Dioxide ◽  
Binding Site ◽  
Coenzyme A ◽  
Structural Data ◽  
Thiamine Pyrophosphate ◽  
Sequence Motifs ◽  
Moorella Thermoacetica ◽  
Microbial Enzyme ◽  
Pyruvate:Ferredoxin Oxidoreductase ◽  
Domain Iii

Pyruvate:ferredoxin oxidoreductase (PFOR) is a microbial enzyme that uses thiamine pyrophosphate (TPP), three [4Fe-4S] clusters, and coenzyme A (CoA) in the reversible oxidation of pyruvate to generate acetyl-CoA and carbon dioxide. The two electrons that are generated as a result of pyruvate decarboxylation are used in the reduction of low potential ferredoxins, which provide reducing equivalents for central metabolism, including the Wood–Ljungdahl pathway. PFOR is a member of the 2-oxoacid:ferredoxin oxidoreductase (OFOR) superfamily, which plays major roles in both microbial redox reactions and carbon dioxide fixation. Here, we present a set of crystallographic snapshots of the best-studied member of this superfamily, the PFOR from Moorella thermoacetica (MtPFOR). These snapshots include the native structure, those of lactyl-TPP and acetyl-TPP reaction intermediates, and the first of an OFOR with CoA bound. These structural data reveal the binding site of CoA as domain III, the function of which in OFORs was previously unknown, and establish sequence motifs for CoA binding in the OFOR superfamily. MtPFOR structures further show that domain III undergoes a conformational change upon CoA binding that seals off the active site and positions the thiolate of CoA directly adjacent to the TPP cofactor. These structural findings provide a molecular basis for the experimental observation that CoA binding accelerates catalysis by 105-fold.

scholarly journals Thermodynamics and economic feasibility of acetone production from syngas using the thermophilic production host Moorella thermoacetica

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Stephanie Redl ◽  
Sumesh Sukumara ◽  
Tom Ploeger ◽  
Liang Wu ◽  
Torbjørn Ølshøj Jensen ◽  
...  
Keyword(s):  
Economic Feasibility ◽  
Moorella Thermoacetica ◽  
Production Host

Kinetic modeling of Moorella thermoacetica growth on single and dual-substrate systems

2016 ◽  
Vol 39 (10) ◽  
pp. 1567-1575 ◽  
Author(s):  
Elliott Schmitt ◽  
Renata Bura ◽  
Rick Gustafson ◽  
Mandana Ehsanipour
Keyword(s):  
Kinetic Modeling ◽  
Moorella Thermoacetica ◽  
Dual Substrate

scholarly journals The Complete Genome Sequence of Clostridium aceticum: a Missing Link between Rnf- and Cytochrome-Containing Autotrophic Acetogens

mBio ◽  
2015 ◽  
Vol 6 (5) ◽  
Author(s):  
Anja Poehlein ◽  
Martin Cebulla ◽  
Marcus M. Ilg ◽  
Frank R. Bengelsdorf ◽  
Bettina Schiel-Bengelsdorf ◽  
...  
Keyword(s):  
Electron Transport Chain ◽  
Proton Translocation ◽  
Acetobacterium Woodii ◽  
Content Type ◽  
Moorella Thermoacetica ◽  
Sustainable Products ◽  
Transport Chain ◽  
Acetogenic Bacteria ◽  
Biotechnological Processes ◽  
Clostridium Aceticum

ABSTRACTClostridium aceticumwas the first isolated autotrophic acetogen, converting CO2plus H2or syngas to acetate. Its genome has now been completely sequenced and consists of a 4.2-Mbp chromosome and a small circular plasmid of 5.7 kbp. Sequence analysis revealed major differences from other autotrophic acetogens.C. aceticumcontains an Rnf complex for energy conservation (via pumping protons or sodium ions). Such systems have also been found inC. ljungdahliiandAcetobacterium woodii. However,C. aceticumalso contains a cytochrome, as doesMoorella thermoacetica, which has been proposed to be involved in the generation of a proton gradient. Thus,C. aceticumseems to represent a link between Rnf- and cytochrome-containing autotrophic acetogens. InC. aceticum, however, the cytochrome is probably not involved in an electron transport chain that leads to proton translocation, as no genes for quinone biosynthesis are present in the genome.IMPORTANCEAutotrophic acetogenic bacteria are receiving more and more industrial focus, as CO2plus H2as well as syngas are interesting new substrates for biotechnological processes. They are both cheap and abundant, and their use, if it results in sustainable products, also leads to reduction of greenhouse gases.Clostridium aceticumcan use both gas mixtures, is phylogenetically not closely related to the commonly used species, and may thus become an even more attractive workhorse. In addition, its energy metabolism, which is characterized here, and the ability to synthesize cytochromes might offer new targets for improving the ATP yield by metabolic engineering and thus allow use ofC. aceticumfor production of compounds by pathways that currently present challenges for energy-limited acetogens.

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