Nomenclature Abstract for Clostridium clariflavum Shiratori et al. 2009.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Clostridium Clariflavum

scholarly journals Draft Genome Sequence of the Cellulolytic and Xylanolytic Thermophile Clostridium clariflavum Strain 4-2a

2015 ◽  
Vol 3 (4) ◽  
Author(s):  
Elise A. Rooney ◽  
Kenneth T. Rowe ◽  
Anna Guseva ◽  
Marcel Huntemann ◽  
James K. Han ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Clostridium Clariflavum

scholarly journals Cellulose- and Xylan-Degrading Thermophilic Anaerobic Bacteria from Biocompost

2011 ◽  
Vol 77 (7) ◽  
pp. 2282-2291 ◽  
Author(s):  
M. V. Sizova ◽  
J. A. Izquierdo ◽  
N. S. Panikov ◽  
L. R. Lynd
Keyword(s):  
Enrichment Culture ◽  
Cellulose Degradation ◽  
Phylogenetic Analyses ◽  
Glycosyl Hydrolase ◽  
Anaerobic Bacteria ◽  
Xylanase Activity ◽  
Cellulase Activity ◽  
Lag Phase ◽  
Fermentation Products ◽  
Clostridium Clariflavum

ABSTRACTNine thermophilic cellulolytic clostridial isolates and four other noncellulolytic bacterial isolates were isolated from self-heated biocompost via preliminary enrichment culture on microcrystalline cellulose. All cellulolytic isolates grew vigorously on cellulose, with the formation of either ethanol and acetate or acetate and formate as principal fermentation products as well as lactate and glycerol as minor products. In addition, two out of nine cellulolytic strains were able to utilize xylan and pretreated wood with roughly the same efficiency as for cellulose. The major products of xylan fermentation were acetate and formate, with minor contributions of lactate and ethanol. Phylogenetic analyses of 16S rRNA and glycosyl hydrolase family 48 (GH48) gene sequences revealed that two xylan-utilizing isolates were related to aClostridium clariflavumstrain and represent a distinct novel branch within the GH48 family. Both isolates possessed high cellulase and xylanase activity induced independently by either cellulose or xylan. Enzymatic activity decayed after growth cessation, with more-rapid disappearance of cellulase activity than of xylanase activity. A mixture of xylan and cellulose was utilized simultaneously, with a significant synergistic effect observed as a reduction of lag phase in cellulose degradation.

scholarly journals Efficient biomass saccharification using a novel cellobiohydrolase from Clostridium clariflavum for utilization in biofuel industry

RSC Advances ◽  
2021 ◽  
Vol 11 (16) ◽  
pp. 9246-9261
Author(s):  
Asma Zafar ◽  
Muhammad Nauman Aftab ◽  
Anam Asif ◽  
Ahmet Karadag ◽  
Liangcai Peng ◽  
...  
Keyword(s):  
Plant Biomass ◽  
Biomass Saccharification ◽  
Biofuel Industry ◽  
Clostridium Clariflavum ◽  
Successful Expression

Successful expression of a novel cellobiohydrolase enzyme from Clostridium clariflavum with efficient saccharification potential of plant biomass for the biofuel industry.

scholarly journals Correction: Efficient biomass saccharification using a novel cellobiohydrolase from Clostridium clariflavum for utilization in biofuel industry

RSC Advances ◽  
2021 ◽  
Vol 11 (19) ◽  
pp. 11387-11387
Author(s):  
Asma Zafar ◽  
Muhammad Nauman Aftab ◽  
Anam Asif ◽  
Ahmet Karadag ◽  
Liangcai Peng ◽  
...  
Keyword(s):  
Biomass Saccharification ◽  
Biofuel Industry ◽  
Clostridium Clariflavum

Correction for ‘Efficient biomass saccharification using a novel cellobiohydrolase from Clostridium clariflavum for utilization in biofuel industry’ by Asma Zafar et al., RSC Adv., 2021, 11, 9246–9261, DOI: 10.1039/D1RA00545F.

scholarly journals A New Subfamily of Glycoside Hydrolase Family 30 with Strict Xylobiohydrolase Function

2021 ◽  
Vol 8 ◽  
Author(s):  
Casey Crooks ◽  
Nathan J. Bechle ◽  
Franz J. St John
Keyword(s):  
Glycoside Hydrolase ◽  
Glucuronic Acid ◽  
Commercial Production ◽  
Glycoside Hydrolase Family ◽  
Biophysical Parameters ◽  
Reaction Conditions ◽  
Hydrolysis Products ◽  
Clostridium Clariflavum ◽  
Hydrolase Family ◽  
Glycoside Hydrolase Family 30

The Acetivibrioclariflavus (basonym: Clostridium clariflavum) glycoside hydrolase family 30 cellulosomal protein encoded by the Clocl_1795 gene was highly represented during growth on cellulosic substrates. In this report, the recombinantly expressed protein has been characterized and shown to be a non-reducing terminal (NRT)-specific xylobiohydrolase (AcXbh30A). Biochemical function, optimal biophysical parameters, and phylogeny were investigated. The findings indicate that AcXbh30A strictly cleaves xylobiose from the NRT up until an α-1,2-linked glucuronic acid (GA)-decorated xylose if the number of xyloses is even or otherwise a single xylose will remain resulting in a penultimate GA-substituted xylose. Unlike recently reported xylobiohydrolases, AcXbh30A has no other detectable hydrolysis products under our optimized reaction conditions. Sequence analysis indicates that AcXbh30A represents a new GH30 subfamily. This new xylobiohydrolase may be useful for commercial production of industrial quantities of xylobiose.

scholarly journals Cellulosomics of the cellulolytic thermophile Clostridium clariflavum

2014 ◽  
Vol 7 (1) ◽  
pp. 100 ◽  
Author(s):  
Lior Artzi ◽  
Bareket Dassa ◽  
Ilya Borovok ◽  
Melina Shamshoum ◽  
Raphael Lamed ◽  
...  
Keyword(s):  
Clostridium Clariflavum
Sign in / Sign up

Export Citation Format

Share Document