Nomenclature Abstract for Rhizobium loti Jarvis et al. 1982.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
George M Garrity
Keyword(s):  
2018 ◽  
Vol 2 (4) ◽  
pp. 44-56

The object of the study is a preparation of ecotoxicant destructor based on bacterial strains of Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas délhiensis and associations of nodule bacteria Rhizobium loti with Lotus corniculatus. It is established, that during the simultaneous use of the bacteria and the legume Lotus corniculatus the rate of oxidation of petroleum hydrocarbons increases three times in comparison with the degradation rate of the pollutant after the separate use of each bacterial strain. We have received the preparation of ecotoxicant destructor – dry heterogeneous mass consisting of viable microbial cells of strains of P. delhiensis and R. lotus, seeds of leguminous plant Lotus corniculatus and delignified sawdust. Oil destructive activity of the preparation is proved in the course of microfield experiments. The degradation of ecotoxicants is proved by capillary gas chromatography-mass spectrometry with mass-selective detection. Bacteria of the strains, included in the preparation, are non-pathogenic for humans and animals, biocompatible, environmentally safe, stable, unpretentious to nutritional needs, technological, do not persist in environmental objects in the absence of a substrate for destruction. The technology of the production of the preparation is developed. The results of these studies demonstrate the possibility of practical use of the degradative potential of the preparation in the course of the implementation of the measures for soil reclamation, cleaning up ecotoxicants and restoring its ecological status.


1994 ◽  
Vol 34 (3) ◽  
pp. 385 ◽  
Author(s):  
RR Gault ◽  
A Pilka ◽  
DM Hebb ◽  
J Brockwell

Strains of rhizobia were isolated from soil around the roots of tagasaste (Chamaecytisus palmensis) growing at 15 widely separated locations in south-eastem Australia. A further collection of strains of both Rhizobium loti and Bradyrhizobium sp. (Lotus) was assembled from 18 legumes including Lotus and other species symbiotically related to Lotus. The strains were used to inoculate tagasaste and 4 species of Lotus in experiments conducted under bacteriologically controlled conditions in a temperature-controlled glasshouse. Tagasaste formed nodules and fixed N2 with all of its homologous rhizobia but there was a wide range of effectiveness among the 15 strains. Tagasaste also formed nodules with each of the 18 strains from other species but fixed N2 with only 10. Four species of Lotus were inoculated with 3 tagasaste strains. One strain nodulated each species and fixed N2 with L. conimhricensis and L. corniculatus but not with L. parviflorus or L. pedunculatus. A second tagasaste strain formed nodules with all 4 Lotus spp. but did not fix N2, while the third nodulated only L. pedunculatus but did not fix N2. A pattern analysis based on the nodulating ability of the host plants in association with 21 strains showed that tagasaste and L. corniculatus formed 1 symbiotic group, and the other 3 Lotus species formed a third group. The pattern analysis procedure based on nodulating capacity of 21 rhizobial strains in association with the 5 host species indicated substantial symbiotic diversity within the collection, with the strains comprising 8 different symbiotic groups. No strain was highly effective on both tagasaste and any of the 4 species of Lotus. Data were insufficient to classify the root-nodule bacteria of tagasaste as either Rhizobium loti or Bradyrhizobium sp. (Lotus).


Author(s):  
B. D. W. Jarvis ◽  
P. Van Berkum ◽  
W. X. Chen ◽  
S. M. Nour ◽  
M. P. Fernandez ◽  
...  
Keyword(s):  

2004 ◽  
Vol 31 (3) ◽  
pp. 122-126 ◽  
Author(s):  
M. J. Estrella ◽  
F. L. Pieckenstain ◽  
M. Marina ◽  
L. E. D�az ◽  
O. A. Ruiz

1997 ◽  
Vol 43 (2) ◽  
pp. 164-177 ◽  
Author(s):  
S. Sivakumaran ◽  
B. D. W. Jarvis ◽  
P. J. Lockhart

A hundred strains of non-nodulating, Gram-negative, rod-shaped bacteria were isolated from clover–ryegrass pastures on three different soil types and from a sandy loam under lupins. When crossed with Escherichia coli PN200 containing the cointegrate plasmid pPN1, 11 transconjugants gained the ability to form nodules on the roots of white clover (Trifolium repens cv. Grasslands Huia). A nodA probe indicated that they had gained nodulation genes. The identities of these 11 strains and 4 others derived from earlier work on non-nodulating root nodule bacteria, were determined by ribotyping, DNA – DNA hybridization, and partial 16S rRNA sequencing. Good agreement was obtained between the three methods, and 11 of the strains were identified as Rhizobium leguminosarum (6), Rhizobium loti (2), Rhizobium etli (1), Rhizobium tropici (1), and Sinorhizobium meliloti (1). DNA –DNA hybridization indicated that the remaining four strains were related to the Rhizobium leguminosarum reference strains. The existence of several species of non-nodulating rhizobia in pasture soil, including species for which the normal host plant was absent, is discussed in relation to the fate of symbiotic plasmids from Rhizobium seed inoculants. It is also suggested that new species should be named for the geographical region from which they are first isolated rather than the host plant.Key words: Rhizobium, non-nodulating, nonsymbiotic, isolation, identification.


1991 ◽  
Vol 57 (5) ◽  
pp. 1563-1565 ◽  
Author(s):  
J. R. Rao ◽  
N. D. Sharma ◽  
J. T. G. Hamilton ◽  
D. R. Boyd ◽  
J. E. Cooper
Keyword(s):  

2006 ◽  
Vol 15 (4) ◽  
pp. 627-638 ◽  
Author(s):  
Isabel M. López-Lara ◽  
Jorrit D. J. Berg ◽  
Jane E. Thomas-Oates ◽  
John Glushka ◽  
Ben J. J. Lugtenberg ◽  
...  

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