Exemplar Abstract for Rhodococcus opacus Klatte et al. 1995.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Rhodococcus Opacus

Exemplar Abstract for Rhodococcus opacus Klatte et al. 1995.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Rhodococcus Opacus

Nomenclature Abstract for Rhodococcus opacus Klatte et al. 1995.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity
Keyword(s):  
Rhodococcus Opacus

Engineering l-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production

2015 ◽  
Vol 30 ◽  
pp. 89-95 ◽  
Author(s):  
Kazuhiko Kurosawa ◽  
Jens Plassmeier ◽  
Jörn Kalinowski ◽  
Christian Rückert ◽  
Anthony J. Sinskey
Keyword(s):  
Rhodococcus Opacus ◽  
Fuel Production ◽  
Lignocellulosic Fuel

scholarly journals Genome Analysis of Rhodococcus Sp. DSSKP-R-001: A Highly Effective β-Estradiol-Degrading Bacterium

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Hongyan Zhao ◽  
Kejian Tian ◽  
Qing Qiu ◽  
Yu Wang ◽  
Hongyan Zhang ◽  
...  
Keyword(s):  
Sole Source ◽  
Rhodococcus Opacus ◽  
Whole Genome ◽  
Metabolic Potential ◽  
Protein Coding ◽  
Degrading Enzymes ◽  
Degrading Bacterium ◽  
Rhodococcus Jostii Rha1 ◽  
Multiple Strains ◽  
Rhodococcus Sp

We screened bacteria that use E2 as its sole source of carbon and energy for growth and identified them as Rhodococcus, and we named them DSSKP-R-001. For a better understanding of the metabolic potential of the strain, whole genome sequencing of Rhodococcus DSSKP-R-001 and annotation of the functional genes were performed. The genomic sketches included a predicted protein-coding gene of approximately 5.4 Mbp with G + C content of 68.72% and 5180. The genome of Rhodococcus strain DSSKP-R-001 consists of three replicons: one chromosome and two plasmids of 5.2, 0.09, and 0.09, respectively. The results showed that there were ten steroid-degrading enzymes distributed in the whole genome of the strain. The existence and expression of estradiol-degrading enzymes were verified by PCR and RTPCR. Finally, comparative genomics was used to compare multiple strains of Rhodococcus. It was found that Rhodococcus DSSKP-R-001 had the highest similarity to Rhodococcus sp. P14 and there were 2070 core genes shared with Rhodococcus sp. P14, Rhodococcus jostii RHA1, Rhodococcus opacus B4, and Rhodococcus equi 103S, showing evolutionary homology. In summary, this study provides a comprehensive understanding of the role of Rhodococcus DSSKP-R-001 in estradiol-efficient degradation of these assays for Rhodococcus. DSSKP-R-001 in bioremediation and evolution within Rhodococcus has important meaning.

Rhodococcus opacus sp.nov., An Unusual Nutritionally Versatile Rhodococcus-species

1994 ◽  
Vol 17 (3) ◽  
pp. 355-360 ◽  
Author(s):  
Stefan Klatte ◽  
Reiner Michael Kroppenstedt ◽  
Frederick A. Rainey
Keyword(s):  
Rhodococcus Opacus ◽  
Rhodococcus Species

scholarly journals The Rhodococcus opacus PD630 Heparin-Binding Hemagglutinin Homolog TadA Mediates Lipid Body Formation

2010 ◽  
Vol 76 (21) ◽  
pp. 7217-7225 ◽  
Author(s):  
Daniel P. MacEachran ◽  
M. E. Prophete ◽  
A. J. Sinskey
Keyword(s):  
Kinetic Studies ◽  
Lipid Body ◽  
Dry Weight ◽  
Rhodococcus Opacus ◽  
Lipid Bodies ◽  
Heparin Binding ◽  
Tag Biosynthesis ◽  
Tag Accumulation

ABSTRACT Generally, prokaryotes store carbon as polyhydroxyalkanoate, starch, or glycogen. The Gram-positive actinomycete Rhodococcus opacus strain PD630 is noteworthy in that it stores carbon in the form of triacylglycerol (TAG). Several studies have demonstrated that R. opacus PD630 can accumulate up to 76% of its cell dry weight as TAG when grown under nitrogen-limiting conditions. While this process is well studied, the underlying molecular and biochemical mechanisms leading to TAG biosynthesis and subsequent storage are poorly understood. We designed a high-throughput genetic screening to identify genes and their products required for TAG biosynthesis and storage in R. opacus PD630. We identified a gene predicted to encode a putative heparin-binding hemagglutinin homolog, which we have termed tadA (triacylglycerol accumulation deficient), as being important for TAG accumulation. Kinetic studies of TAG accumulation in both the wild-type (WT) and mutant strains demonstrated that the tadA mutant accumulates 30 to 40% less TAG than the parental strain (WT). We observed that lipid bodies formed by the mutant strain were of a different size and shape than those of the WT. Characterization of TadA demonstrated that the protein is capable of binding heparin and of agglutinating purified lipid bodies. Finally, we observed that the TadA protein localizes to lipid bodies in R. opacus PD630 both in vivo and in vitro. Based on these data, we hypothesize that the TadA protein acts to aggregate small lipid bodies, found in cells during early stages of lipid storage, into larger lipid bodies and thus plays a key role in lipid body maturation in R. opacus PD630.

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