Exemplar Abstract for Anaerotignum propionicum (Cardon and Barker 1946) Ueki et al. 2017, Clostridium propionicum Cardon and Barker 1946 (Approved Lists 1980) and Tyzzerella propionica (Cardon and Barker 1946) Yutin and Galperin 2013.

2003 ◽  
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2014 ◽  
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pp. 2041-2053 ◽  
Author(s):  
Andreas Heine ◽  
Gloria Herrmann ◽  
Thorsten Selmer ◽  
Felix Terwesten ◽  
Wolfgang Buckel ◽  
...  

2003 ◽  
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Matthias Brock ◽  
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Bernard T. Golding ◽  
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Olga Selifonova ◽  
...  

2017 ◽  
Author(s):  
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Luca Ricci ◽  
Soo Mei Chee ◽  
David Bell ◽  
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...  

ABSTRACTThe polyhydroxyalkanoates are a group of microbially-produced biopolymers that have been proposed as sustainable alternatives to several oil-derived plastics. However, polyhydroxyalkanoates are currently more expensive to produce than oil-derived plastics and therefore, more efficient production processes would be desirable. Cell-free transcription-translation-based metabolic engineering strategies have been previously used to optimise several different biosynthetic pathways but not the polyhydroxyalkanoates biosynthetic pathways. Here we have developed several Escherichia coli cell-free transcription-translation-based systems for in vitro prototyping of polyhydroxyalkanoates biosynthetic operons, and also for screening relevant metabolite recycling enzymes. These cell-free transcription-translation reactions were customised through the addition of whey permeate, an industrial waste that has been previously used as a low-cost feedstock for optimising in vivo polyhydroxyalkanoates production. We found that the inclusion of an optimal concentration of whey permeate enhanced relative cell-free GFPmut3b production by ~20% compared to control reactions that did not include whey permeate. An analysis of pH in our cell-free reactions suggests that the observed increase in GFPmut3b production was likely through enhanced ATP generation, as a consequence of the glycolytic processing of lactose present in whey permeate. We also found that whey permeate enhanced cell-free reactions produced ~3μM (R)-3HB-CoA, whilst, coupled cell-free biotransformation/transcription-translation reactions produced a ten-fold greater yield of (R)-3HB-CoA. These reactions were also used to characterise a Clostridium propionicum propionyl CoA transferase enzyme that can recycle Acetyl-CoA. Together our data demonstrate that cell-free approaches can be used to complement in vivo workflows for identifying additional strategies for optimising polyhydroxyalkanoates production.


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