Exemplar Abstract for Clostridium propionicum Cardon and Barker 1946 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
George M Garrity
Keyword(s):  
Clostridium Propionicum

Biological production of acrylic acid from cheese whey by resting cells of Clostridium propionicum

1990 ◽  
Vol 6 (4) ◽  
pp. 237-242 ◽  
Author(s):  
Dennis J. O'Brien ◽  
Curtis C. Panzer ◽  
William P. Eisele
Keyword(s):  
Acrylic Acid ◽  
Cheese Whey ◽  
Resting Cells ◽  
Biological Production ◽  
Clostridium Propionicum

scholarly journals Nucleotidases of Clostridium propionicum

1960 ◽  
Vol 235 (10) ◽  
pp. 2935-2938
Author(s):  
Earl R. Stadtman ◽  
J.M. Earl ◽  
Elmer B. Brown
Keyword(s):  
Clostridium Propionicum

High resolution crystal structure of Clostridium propionicum β-alanyl-CoA:ammonia lyase, a new member of the “hot dog fold” protein superfamily

2014 ◽  
Vol 82 (9) ◽  
pp. 2041-2053 ◽  
Author(s):  
Andreas Heine ◽  
Gloria Herrmann ◽  
Thorsten Selmer ◽  
Felix Terwesten ◽  
Wolfgang Buckel ◽  
...  
Keyword(s):  
Crystal Structure ◽  
High Resolution ◽  
Protein Superfamily ◽  
Clostridium Propionicum ◽  
Hot Dog

scholarly journals Acryloyl-CoA reductase from Clostridium propionicum

2003 ◽  
Vol 270 (5) ◽  
pp. 902-910 ◽  
Author(s):  
Marc Hetzel ◽  
Matthias Brock ◽  
Thorsten Selmer ◽  
Antonio J. Pierik ◽  
Bernard T. Golding ◽  
...  
Keyword(s):  
Clostridium Propionicum ◽  
Coa Reductase

scholarly journals Two beta-alanyl-CoA:ammonia lyases in Clostridium propionicum

FEBS Journal ◽  
2005 ◽  
Vol 272 (3) ◽  
pp. 813-821 ◽  
Author(s):  
Gloria Herrmann ◽  
Thorsten Selmer ◽  
Holly J. Jessen ◽  
Ravi R. Gokarn ◽  
Olga Selifonova ◽  
...  
Keyword(s):  
Clostridium Propionicum

scholarly journals Cell-free prototyping strategies for enhancing the sustainable production of polyhydroxyalkanoates bioplastics

2017 ◽  
Author(s):  
Richard Kelwick ◽  
Luca Ricci ◽  
Soo Mei Chee ◽  
David Bell ◽  
Alexander J. Webb ◽  
...  
Keyword(s):  
Industrial Waste ◽  
Low Cost ◽  
Efficient Production ◽  
Biosynthetic Pathways ◽  
Whey Permeate ◽  
Coa Transferase ◽  
Clostridium Propionicum ◽  
Atp Generation

ABSTRACTThe polyhydroxyalkanoates are a group of microbially-produced biopolymers that have been proposed as sustainable alternatives to several oil-derived plastics. However, polyhydroxyalkanoates are currently more expensive to produce than oil-derived plastics and therefore, more efficient production processes would be desirable. Cell-free transcription-translation-based metabolic engineering strategies have been previously used to optimise several different biosynthetic pathways but not the polyhydroxyalkanoates biosynthetic pathways. Here we have developed several Escherichia coli cell-free transcription-translation-based systems for in vitro prototyping of polyhydroxyalkanoates biosynthetic operons, and also for screening relevant metabolite recycling enzymes. These cell-free transcription-translation reactions were customised through the addition of whey permeate, an industrial waste that has been previously used as a low-cost feedstock for optimising in vivo polyhydroxyalkanoates production. We found that the inclusion of an optimal concentration of whey permeate enhanced relative cell-free GFPmut3b production by ~20% compared to control reactions that did not include whey permeate. An analysis of pH in our cell-free reactions suggests that the observed increase in GFPmut3b production was likely through enhanced ATP generation, as a consequence of the glycolytic processing of lactose present in whey permeate. We also found that whey permeate enhanced cell-free reactions produced ~3μM (R)-3HB-CoA, whilst, coupled cell-free biotransformation/transcription-translation reactions produced a ten-fold greater yield of (R)-3HB-CoA. These reactions were also used to characterise a Clostridium propionicum propionyl CoA transferase enzyme that can recycle Acetyl-CoA. Together our data demonstrate that cell-free approaches can be used to complement in vivo workflows for identifying additional strategies for optimising polyhydroxyalkanoates production.

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