Exemplar Abstract for Rhizobium fredii Scholla and Elkan 1984, Sinorhizobium fredii (Scholla and Elkan 1984) Chen et al. 1988 emend. De Lajudie et al. 1994 and Ensifer fredii Young 2003 emend. Hördt et al. 2020.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
1997 ◽  
Vol 10 (1) ◽  
pp. 138-141 ◽  
Author(s):  
Jun Gu ◽  
Pedro A. Balatti ◽  
Hari B. Krishnan ◽  
Steven G. Pueppke

The transcripts of nolW and nolB, two divergently oriented cultivar specificity genes of Rhizobium fredii strain USDA257, are known to be initiated 14 bp apart from promoters that face one another. We show here that expression of nolB is dependent both on induction with flavonoid signals and on the regulatory gene, nodD1. Expression of nolW is constitutive and independent of flavonoids and nodD1. Normal expression of nolB is retained with a promoter that extends only 61 bp upstream of the transcript start site, but it is lost if an additional 24 bp are removed. Substantial expression of nolW is retained with a promoter that contains only 34 bp of DNA upstream from the transcript initiation site. The dual control region for the two genes is thus only about 109 bp in length.


1995 ◽  
Vol 17 (5) ◽  
pp. 923-933 ◽  
Author(s):  
Laszlo G. Kovacs ◽  
Pedro A. Balatti ◽  
Hari B. Krishnan ◽  
Steven G. Pueppke
Keyword(s):  

2012 ◽  
Vol 194 (16) ◽  
pp. 4483-4483 ◽  
Author(s):  
J. Schuldes ◽  
M. Rodriguez Orbegoso ◽  
C. Schmeisser ◽  
H. B. Krishnan ◽  
R. Daniel ◽  
...  

1997 ◽  
Vol 34 (1) ◽  
pp. 55-60
Author(s):  
Robert R. Burke ◽  
Diana D. Heard ◽  
Anthony J. Nieuwkoop

2001 ◽  
Vol 33 (6) ◽  
pp. 837-840 ◽  
Author(s):  
L.B. Videira ◽  
G.N. Pastorino ◽  
P.A. Balatti
Keyword(s):  

Author(s):  
Dongzhi Li ◽  
Ziqi Li ◽  
Jing Wu ◽  
Zhide Tang ◽  
Fuli Xie ◽  
...  

Gram-negative bacteria can produce outer membrane vesicles (OMVs), and most functional studies of OMVs have been focused on mammalian-bacterial interactions. However, research on the OMVs of rhizobia is still limited so far. In this work, we isolated and purified OMVs from Sinorhizobium fredii HH103 under free-living conditions that was set as control (C-OMVs) and symbiosis-mimicking conditions that was induced by genistein (G-OMVs). The soybean roots treated with G-OMVs displayed significant deformation of root hairs. G-OMVs significantly induced the expression of nodulation genes related to early symbiosis, while inhibited that of the defense genes of soybean. Proteomics analysis identified a total of 93 differential proteins between C-OMVs and G-OMVs, which are mainly associated with ribosome synthesis, flagellar assembly, two-component system, ABC transporters, oxidative phosphorylation, nitrogen metabolism, quorum sensing, glycerophospholipid metabolism and peptidoglycan biosynthesis. A total of 45 differential lipids were identified in lipidomics analysis. Correlation analysis of OMV proteome and lipidome data revealed that glycerophospholipid metabolism is the enriched KEGG metabolic pathway, and the expression of phosphatidylserine decarboxylase was significantly up-regulated in G-OMVs. The changes in three lipids related to symbiosis in the glycerophospholipid metabolism pathway were verified by ELISA. Our results indicate that glycerophospholipid metabolism contributes to rhizobia-soybean symbiosis via OMVs.


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