scholarly journals The Absence of the N-acyl-homoserine-lactone Autoinducer Synthase Genes traI and ngrI Increases the Copy Number of the Symbiotic Plasmid in Sinorhizobium fredii NGR234

2016 ◽  
Vol 7 ◽  
Author(s):  
Jessica Grote ◽  
Dagmar Krysciak ◽  
Katrin Petersen ◽  
Simon Güllert ◽  
Christel Schmeisser ◽  
...  
Keyword(s):  
Copy Number ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Sinorhizobium Fredii ◽  
Symbiotic Plasmid

scholarly journals A single point mutation converts a glutaryl-7-aminocephalosporanic acid acylase into an N-acyl-homoserine lactone acylase

2021 ◽  
Author(s):  
Shereen A. Murugayah ◽  
Gary B. Evans ◽  
Joel D. A. Tyndall ◽  
Monica L. Gerth
Keyword(s):  
Single Point ◽  
Quorum Quenching ◽  
Homoserine Lactone ◽  
Site Directed Mutagenesis ◽  
Saturation Mutagenesis ◽  
Single Amino Acid ◽  
Single Point Mutation ◽  
Acyl Homoserine Lactone ◽  
Signalling Molecules ◽  
Single Amino Acid Residue

Abstract Objective To change the specificity of a glutaryl-7-aminocephalosporanic acid acylase (GCA) towards N-acyl homoserine lactones (AHLs; quorum sensing signalling molecules) by site-directed mutagenesis. Results Seven residues were identified by analysis of existing crystal structures as potential determinants of substrate specificity. Site-saturation mutagenesis libraries were created for each of the seven selected positions. High-throughput activity screening of each library identified two variants—Arg255Ala, Arg255Gly—with new activities towards N-acyl homoserine lactone substrates. Structural modelling of the Arg255Gly mutation suggests that the smaller side-chain of glycine (as compared to arginine in the wild-type enzyme) avoids a key clash with the acyl group of the N-acyl homoserine lactone substrate. Conclusions Mutation of a single amino acid residue successfully converted a GCA (with no detectable activity against AHLs) into an AHL acylase. This approach may be useful for further engineering of ‘quorum quenching’ enzymes.

n → π* Interactions in N-Acyl Homoserine Lactone Derivatives and Their Effects on Hydrolysis Rates

2019 ◽  
Vol 123 (13) ◽  
pp. 2537-2543 ◽  
Author(s):  
Daniel J. Schmucker ◽  
Sydney R. Dunbar ◽  
Tricia D. Shepherd ◽  
Michael A. Bertucci
Keyword(s):  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Π Interactions ◽  
Hydrolysis Rates

Biofilm Formation, Communication and Interactions of Mesophilic Leaching Bacteria during Pyrite Oxidation

2013 ◽  
Vol 825 ◽  
pp. 107-110
Author(s):  
Sören Bellenberg ◽  
Robert Barthen ◽  
Mario Vera ◽  
Nicolas Guiliani ◽  
Wolfgang Sand
Keyword(s):  
Quorum Sensing ◽  
Acidithiobacillus Ferrooxidans ◽  
Biofilm Formation ◽  
Pyrite Oxidation ◽  
Cell Communication ◽  
Homoserine Lactone ◽  
Mixed Cultures ◽  
Acyl Homoserine Lactone ◽  
Leaching Bacteria ◽  
Leaching Efficiency

A functional luxIR-type Quorum Sensing (QS) system is present in Acidithiobacillus ferrooxidans. However, cell-cell communication among various acidophilic chemolithoautotrophs growing on pyrite has not been studied in detail. These aspects are the scope of this study with emphasis on the effects exerted by the N-acyl-homoserine lactone (AHL) type signaling molecules which are produced by Acidithiobacillus ferrooxidans. Their effects on attachment and leaching efficiency by other leaching bacteria, such as Acidithiobacillus ferrivorans, Acidiferrobacter spp. SPIII/3 and Leptospirillum ferrooxidans in pure and mixed cultures growing on pyrite is shown.

scholarly journals Acyl-homoserine lactone-dependent eavesdropping promotes competition in a laboratory co-culture model

2012 ◽  
Vol 6 (12) ◽  
pp. 2219-2228 ◽  
Author(s):  
Josephine R Chandler ◽  
Silja Heilmann ◽  
John E Mittler ◽  
E Peter Greenberg
Keyword(s):  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
Culture Model

Diversity and N-acyl-homoserine lactone production by Gammaproteobacteria associated with Avicennia marina rhizosphere of South Indian mangroves

2015 ◽  
Vol 38 (5) ◽  
pp. 340-345 ◽  
Author(s):  
Ganga Viswanath ◽  
Sekar Jegan ◽  
Viswanathan Baskaran ◽  
Raju Kathiravan ◽  
Vaiyapuri Ramalingam Prabavathy
Keyword(s):  
Avicennia Marina ◽  
Homoserine Lactone ◽  
Acyl Homoserine Lactone ◽  
South Indian ◽  
Indian Mangroves
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