scholarly journals Development and characterization of an immunoaffinity column for the detection of danofloxacin residues in milk samples

2019 ◽  
Vol 39 (2) ◽  
pp. 500-506 ◽  
Author(s):  
Rui NIU ◽  
Yamei YANG ◽  
Yadan WANG ◽  
Shilong LUO ◽  
Cheng ZHANG ◽  
...  
Keyword(s):  
Immunoaffinity Column ◽  
Milk Samples

Occurrence and characterization of ciprofloxacin‐resistant Escherichia coli from bovine and ovine bulk tank milk samples in Turkey

2021 ◽  
Author(s):  
Cemil Kürekci ◽  
Muhsin Aydın ◽  
İbrahim Ozan Tekeli ◽  
Pınar Ambarcıoğlu ◽  
Seydi Ahmet Şengül ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank

Characterization of the microbiota and resistome of bulk tank milk samples from Prince Edward Island dairy farms

2021 ◽  
Author(s):  
Landon M.C. Warder ◽  
Enrique Doster ◽  
Jennifer K. Parker ◽  
Paul S. Morley ◽  
J.T. McClure ◽  
...  
Keyword(s):  
Prince Edward Island ◽  
Dairy Farms ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank

scholarly journals MOLECULAR DETECTION AND CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM RAW MILK SOLD IN DIFFERENT MARKETS OF BANGLADESH

2017 ◽  
Vol 14 (2) ◽  
pp. 277-282 ◽  
Author(s):  
M. A. Islam ◽  
S. M. L. Kabir ◽  
M. T. Rahman
Keyword(s):  
Molecular Detection ◽  
Culture Media ◽  
Raw Milk ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Selective Media ◽  
Milk Samples ◽  
Antimicrobial Sensitivity ◽  
Antimicrobial Sensitivity Test

The study was intended for molecular detection of S. aureus isolated from raw cow’s milk. A total of 20 milk samples were collected from different upazila markets of Jamalpur, Tangail, Kishoreganj and Netrokona districts of Bangladesh. Milk samples were cultured onto various culture media for the isolation of bacteria. The isolated bacteria were identified by studying cultural properties on different selective media, biochemical tests, and finally by PCR. Out of 20 samples, 15 (75%) milk samples were found to be positive for S. aureus. S. aureus specific 16S rRNA gene was amplified from all isolates and identified as S. aureus. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the S. aureus isolates were resistant to amoxicillin (100%), erythromycin (73.33%) and tetracycline (73.33%) but sensitive to azithromycin (93.33%), ciprofloxacin (93.33%), gentamicin (100%), norfloxacin (86.67%) and streptomycin (86.67%).

scholarly journals Isolation and molecular characterization of Mycobacterium tuberculosis complex isolated from raw milk in some dairy farms in Egypt

2016 ◽  
Vol 5 (2) ◽  
pp. 105
Author(s):  
Heba Hussien ◽  
Eman Mahrous
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Culture Method ◽  
Raw Milk ◽  
Accurate Method ◽  
Tuberculosis Complex ◽  
Milk Samples ◽  
Source Of Infection

<p>This study was conducted to detect <em>Mycobacterium tuberculosis</em> complex in milk in three Egyptian Governorates; El-Sharkia, El-Menoufia and El-Behera Governorates. 300 milk samples were collected from tuberculin positive cases, 18 (6.0%) were shedding <em>Mycobacterium tuberculosis</em> complex in their milk which detected by real time PCR. On another hand, 170 milk samples were collected from tuberculin negative cases, 5 (2.9%) were shedding <em>Mycobacterium tuberculosis</em> complex in their milk which detected by real time PCR. All milk samples were examined by three techniques including Microscopic examination, culture and real time PCR. Real time PCR is more rapid and accurate method than microscopic and culture method. The isolated colonies from culture were examined by Multiplex PCR to demonstrate the source of infection either human or animal source.</p>

scholarly journals Antimicrobial resistance and genetic characterization of coagulase-negative staphylococci from bovine mastitis milk samples in Korea

2019 ◽  
Vol 102 (12) ◽  
pp. 11439-11448 ◽  
Author(s):  
Su-Jeong Kim ◽  
Dong Chan Moon ◽  
Seung-Chun Park ◽  
Hee Young Kang ◽  
Seok Hyeon Na ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Genetic Characterization ◽  
Bovine Mastitis ◽  
Coagulase Negative Staphylococci ◽  
Milk Samples

Preparation and characterization of an immunoaffinity column for the selective extraction of aflatoxin B1 in 13 kinds of foodstuffs

2015 ◽  
Vol 998-999 ◽  
pp. 50-56 ◽  
Author(s):  
Jie Xie ◽  
Tao Peng ◽  
Jian-Li He ◽  
Yu Shao ◽  
Chun-Lin Fan ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Selective Extraction ◽  
Immunoaffinity Column

scholarly journals Genotypic Characterization of Coagulase-negative Staphylococci Isolated from Sheep Milk in Slovakia

2010 ◽  
Vol 79 (2) ◽  
pp. 269-275 ◽  
Author(s):  
Ivana Pilipčincová ◽  
Mangesh Bhide ◽  
Eva Dudriková ◽  
Milan Trávniček
Keyword(s):  
Molecular Characterization ◽  
Gel Electrophoresis ◽  
Biochemical Characterization ◽  
Pulse Field ◽  
Coagulase Negative Staphylococci ◽  
Pulse Field Gel Electrophoresis ◽  
Milk Samples ◽  
Sheep Milk ◽  
Epidemiological Situation

Hitherto very few reports are available presenting identification and molecular characterization of the coagulase negative staphylococci (CNS) from sheep milk in the subclinical stage of mastitis. Furthermore, very scanty data are available on the epidemiological status of CNS in different Slovak provinces. Milk samples from 54 sheep farms located in eastern Slovak region were screened. A total 240 CNS were identified with series of biochemical testes (STAPH-API) and subjected further for genotyping with the help of pulse field gel electrophoresis (PFGE). The most frequently occurring CNS species according the biochemical characterization were:S. epidermidis(36.3 %),S. caprae(21.3 %),S. hominis(6.6 %),S. chromogenes(6.3 %),S. xylosus(5.8 %),S. warneri(5.0 %) andS. capitis(4.6 %). Further PFGE-based characterization of these isolates revealed six pulsotypes of theS. epidermidis, two ofS. caprae, three ofS. chromogenes, nine ofS. hominis, five ofS. capitisand seven ofS. xylosus. These results contribute to knowledge of the epidemiological situation of the CNS from the subclinical form of mastitis in Slovakia.

Microbiological Characterization of Human Milk1

1977 ◽  
Vol 40 (9) ◽  
pp. 614-616 ◽  
Author(s):  
A. GAVIN ◽  
K. OSTOVAR
Keyword(s):  
Staphylococcus Aureus ◽  
Breast Feeding ◽  
Staphylococcus Epidermidis ◽  
Lactobacillus Acidophilus ◽  
Bacterial Population ◽  
Streptococcus Salivarius ◽  
Streptococcus Mitis ◽  
Bacterial Populations ◽  
Milk Samples

Milk samples of five breast-feeding mothers were studied for bacterial population, flora, and source. In most instances, samples taken at postfeeding contained higher bacterial populations than prefeeding samples. Staphylococcus epidermidis was the predominant organism isolated from 100% of the samples. Increases were noticed in the appearance of Streptococcus mitis, Gaffkya tetragena, Streptococcus salivarius, Staphylococcus aureus, as well as Lactobacillus acidophilus, in the postfeeding samples. Main sources of bacteria were found to be the infant's mouth and maternal skin.

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