scholarly journals Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea

2018 ◽  
Vol 40 (1) ◽  
Author(s):  
NAYARA NIELE SACOMAN ◽  
ALEXANDRE PIO VIANA ◽  
VIRGINIA SILVA CARVALHO ◽  
EILEEN AZEVEDO SANTOS ◽  
ROSANA RODRIGUES
Keyword(s):  
Mosaic Virus ◽  
Elisa Test ◽  
Passion Fruit ◽  
The Other ◽  
Visual Evaluation ◽  
Breeding Programs ◽  
Genetic Breeding ◽  
Fruit Species ◽  
Cowpea Aphid

ABSTRACT Passion fruit (Passiflora edulis) is a fruit species of great relevance for Brazilian economy. However, it is highly susceptible to the Cowpea aphid-borne mosaic virus (CABMV). The species P.setacea, on the other hand, is resistant to the disease. The present study aimed to identify CABMV-resistant P. setacea genotypes for the introgression of genes into sour passion fruit genetic breeding programs. The seeds of passion fruit genotypes were germinated in vitro in MS culture medium. The seedlings were acclimatized in a growth chamber at the temperature of 27 ± 2ºC, photoperiod of 16:8 hours and 80% of relative humidity. Thirty plants of each genotype were mechanically inoculated with extract prepared from leaves collected from passion fruit plants with symptoms of CABMV for the assessment of resistance to CABMV. The severity of leaf symptoms was evaluated by means of a grading scale of visual signs. After the visual evaluation and identification of the asymptomatic genotypes of P. setacea, the PTA-ELISA test was carried out for 30 selected genotypes. According to the visual evaluation, all P. setacea genotypes were highly resistant to CABMV, while the P. edulis genotypes were highly susceptible. Out of the 30 genotypes selected, only PsRJ 4 was considered susceptible by PTA-ELISA. The other genotypes of P. setacea were considered resistant and present great potential for use in passion fruit genetic breeding programs.

scholarly journals Interaction between Cowpea Aphid-Borne Mosaic Virus Isolates and Its Effect on Passion Fruit Woodiness Disease on Passiflora edulis Sims and Passiflora ligularis Juss

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Lucy J. Kiptui ◽  
Faith J. Toroitich ◽  
Dora C. Kilalo ◽  
Meshack Obonyo
Keyword(s):  
Mosaic Virus ◽  
Elisa Test ◽  
Synergistic Interaction ◽  
Passion Fruit ◽  
Economic Losses ◽  
Passiflora Edulis ◽  
Cowpea Aphid ◽  
Virus Isolates ◽  
Screen House ◽  
Viral Isolates

In East Africa, passion fruit woodiness disease is caused by potyviruses, among which are Cowpea Aphid-Borne Mosaic Virus (CABMV) and Uganda Passiflora Virus (UPV). Previous studies suggest that synergistic interaction of viruses causes mild or severe outcomes of the disease. However, mixed infections of these viruses have not been documented. The aim of this study was to determine the effects of the interaction between two CABMV isolates causing passion fruit woodiness disease in Kenya. Healthy plants were mechanically inoculated at six-leaf stage. Four treatments were used: CABMV isolate 1, CABMV isolate 2, mixture of the two isolates, and noninoculated control. The test plants were maintained in a screen house, and data on symptom severity and rate of disease development were recorded. ELISA test was conducted to confirm virus presence and distribution. The results indicate that the CABMV isolates used are systemic. There were various differences in disease progression, which was faster in the sweet passion variety than in the purple variety. However, the latter had a higher disease severity. Coinfected plants expressed severe symptoms compared to the singly infected ones, indicating synergistic interaction between the viral isolates. This implies that coinfection has adverse impact resulting in higher economic losses.

scholarly journals Yellow passion fruit reaction to Xanthomonas axonopodis pv. passiflorae and to Cowpea aphid-borne mosaic virus

2018 ◽  
Vol 18 (4) ◽  
pp. 349-356
Author(s):  
Anne Pinheiro Costa ◽  
Isadora Nogueira ◽  
José Ricardo Peixoto ◽  
Michelle de Souza Vilela ◽  
Luiz Eduardo Bassay Blum ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Passion Fruit ◽  
Xanthomonas Axonopodis ◽  
Cowpea Aphid ◽  
Yellow Passion Fruit

scholarly journals Transcriptome Sequencing Reveals a Complete Genome Sequence of Cowpea Aphid-Borne Mosaic Virus from Passion Fruit in Kenya

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
F. Munguti ◽  
S. Maina ◽  
E. N. Nyaboga ◽  
D. Kilalo ◽  
E. Kimani ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Transcriptome Sequencing ◽  
Passion Fruit ◽  
Rna Seq ◽  
Cowpea Aphid

Analysis of transcriptome sequencing (RNA-Seq) data revealed a complete Cowpea aphid-borne mosaic virus (CABMV) genome from virus-infected passion fruit in Kenya. We compared it with six complete CABMV genomes, one each from Zimbabwe and Uganda and two each from Brazil and India.

Yellow passion fruit genotypes reaction to woodiness disease (Cowpea aphid-borne mosaic virus) in field conditions

2020 ◽  
pp. 427-432
Author(s):  
G.S. Miranda ◽  
O.K. Yamanishi ◽  
J.R. Peixoto ◽  
M. de S. Vilella ◽  
M. de C. Pires ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Passion Fruit ◽  
Field Conditions ◽  
Cowpea Aphid ◽  
Yellow Passion Fruit

Genetic transformation of sweet passion fruit (Passiflora alata) and reactions of the transgenic plants to Cowpea aphid borne mosaic virus

2015 ◽  
Vol 143 (4) ◽  
pp. 813-821 ◽  
Author(s):  
Marcelo Favareto Correa ◽  
Ana Paula Chiaverini Pinto ◽  
Jorge Alberto Marques Rezende ◽  
Ricardo Harakava ◽  
Beatriz Madalena Januzzi Mendes
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Mosaic Virus ◽  
Passion Fruit ◽  
Cowpea Aphid

scholarly journals Screening of Passiflora species for reaction to Cowpea aphid-borne mosaic virus reveals an immune wild species

2009 ◽  
Vol 66 (3) ◽  
pp. 414-418 ◽  
Author(s):  
Scheila da Conceição Maciel ◽  
Daniel Hiroshi Nakano ◽  
Jorge Alberto Marques Rezende ◽  
Maria Lúcia Carneiro Vieira
Keyword(s):  
Mosaic Virus ◽  
Wild Species ◽  
Virus Disease ◽  
Passion Fruit ◽  
Mechanical Inoculation ◽  
Fruit Crops ◽  
Rt Pcr ◽  
Cowpea Aphid ◽  
Virus Isolates

Cowpea aphid-borne mosaic virus (CABMV) is a potyvirus that causes the most serious virus disease of passion fruit crops in Brazil. It is transmitted by several species of aphids in a non-persistent, non-circulative manner. The reaction of 16 species of Passiflora to infection by mechanical inoculation with four Brazilian isolates of CABMV was evaluated under greenhouse conditions. Only P. suberosa, a wild species, was resistant to infection by all virus isolates, in two independent assays. P. suberosa grafted onto infected P. edulis f. flavicarpa did not develop symptoms; neither was the virus detected by RT-PCR in the upper leaves, suggesting that this species is immune to CABMV.

Effect of vegetable oil on the efficiency of transmission of cowpea aphid-borne mosaic virus by Aphis gossypii Glover in passion fruit plants

2021 ◽  
Author(s):  
Daniel Remor Moritz ◽  
Neemias da Silva Santos ◽  
Francis Zanini ◽  
Bruna Alana Pacini ◽  
Henrique Belmonte Petry ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Vegetable Oil ◽  
Aphis Gossypii ◽  
Passion Fruit ◽  
Aphis Gossypii Glover ◽  
Cowpea Aphid ◽  
Fruit Plants

scholarly journals Temporal and spatial progress of Cowpea aphid-borne mosaic virus (CABMV) in passion fruit (Passiflora edulis Sims)

2021 ◽  
pp. 284-289
Author(s):  
Roger Yochiharu Kotsubo ◽  
Karina Silva dos Santos ◽  
Lucas Henrique Fantin ◽  
Vitória Carolina Antunes Chaves ◽  
João Valdecir Casaroto Filho ◽  
...  
Keyword(s):  
Spatial Pattern ◽  
Mosaic Virus ◽  
Aphis Gossypii ◽  
Management Strategies ◽  
Aphid Species ◽  
Passion Fruit ◽  
Aphis Fabae ◽  
Incidence Data ◽  
Cowpea Aphid ◽  
Aphid Vectors

Among the diseases that affect passion fruit, those causing fruit woodiness is considered the most important. Cowpea aphid-borne mosaic virus (CABMV) is an important vírus that is transmitted by several aphid species during the bite, making the use of insecticides infeasible to control these vectors. In order to understand the epidemiological behavior of the disease, this study aimed to study the temporal progress and spatial pattern of CABMV in the field. Healthy seedlings of passion fruit with 2.0 meters height were implanted in the field condition at the experimental station of the IDR-Paraná, Brazil. The evaluations were performed weekly observing the symptoms of blister, mosaic, chlorosis and crinkled leaves. For the analyses, the logistic, gompertz and monomolecular models were adjusted to CABMV incidence data in passion fruit. The spatial pattern of the disease was characterized by the dispersion index and Taylor's Power Law. The logistic model was the one that best described the progress in the incidence of the disease. The incidence progress rate of CABMV was 0.037, 0.077 and 0.060 % day-1. At the beginning of the epidemic, the pattern was random. The initial dispersion mechanism was occured through aphid vectors, como Aphis gossypii Glover, Aphis fabae Scopoli, Aphis solanella Theobald, Toxoptera citricida Kilkaldy, Uroleucon ambrosiae Thomas and Uroleucon sonchi L. infected with CABMV that starts its test bite randomly. The pattern of disease dispersion began to be added when the incidence of plants reached 10 and 7%, in the 2015/16 and 2017/18 harvests, respectively. The random spatial pattern suggested that infected aphids enter several points of the orchard and infected plants become a source for secondary infections, characterizing aggregate pattern. Thus, the eradication of alternative hosts abroad can be adopted as management strategies of CABMV

Sour passion fruit breeding: Strategy applied to individual selection in segregating population of Passiflora resistant to Cowpea aphid-born mosaic virus (CABMV)

2016 ◽  
Vol 211 ◽  
pp. 241-247 ◽  
Author(s):  
Jôsie Cloviane de Oliveira Freitas ◽  
Alexandre Pio Viana ◽  
Eileen Azevedo Santos ◽  
Claudia Lougon Paiva ◽  
Fernando Higino de Lima e Silva ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Breeding Strategy ◽  
Passion Fruit ◽  
Individual Selection ◽  
Fruit Breeding ◽  
Cowpea Aphid ◽  
Segregating Population ◽  
Sour Passion Fruit
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