scholarly journals Interaction between Cowpea Aphid-Borne Mosaic Virus Isolates and Its Effect on Passion Fruit Woodiness Disease on Passiflora edulis Sims and Passiflora ligularis Juss

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Lucy J. Kiptui ◽  
Faith J. Toroitich ◽  
Dora C. Kilalo ◽  
Meshack Obonyo
Keyword(s):  
Mosaic Virus ◽  
Elisa Test ◽  
Synergistic Interaction ◽  
Passion Fruit ◽  
Economic Losses ◽  
Passiflora Edulis ◽  
Cowpea Aphid ◽  
Virus Isolates ◽  
Screen House ◽  
Viral Isolates

In East Africa, passion fruit woodiness disease is caused by potyviruses, among which are Cowpea Aphid-Borne Mosaic Virus (CABMV) and Uganda Passiflora Virus (UPV). Previous studies suggest that synergistic interaction of viruses causes mild or severe outcomes of the disease. However, mixed infections of these viruses have not been documented. The aim of this study was to determine the effects of the interaction between two CABMV isolates causing passion fruit woodiness disease in Kenya. Healthy plants were mechanically inoculated at six-leaf stage. Four treatments were used: CABMV isolate 1, CABMV isolate 2, mixture of the two isolates, and noninoculated control. The test plants were maintained in a screen house, and data on symptom severity and rate of disease development were recorded. ELISA test was conducted to confirm virus presence and distribution. The results indicate that the CABMV isolates used are systemic. There were various differences in disease progression, which was faster in the sweet passion variety than in the purple variety. However, the latter had a higher disease severity. Coinfected plants expressed severe symptoms compared to the singly infected ones, indicating synergistic interaction between the viral isolates. This implies that coinfection has adverse impact resulting in higher economic losses.

scholarly journals Screening of Passiflora species for reaction to Cowpea aphid-borne mosaic virus reveals an immune wild species

2009 ◽  
Vol 66 (3) ◽  
pp. 414-418 ◽  
Author(s):  
Scheila da Conceição Maciel ◽  
Daniel Hiroshi Nakano ◽  
Jorge Alberto Marques Rezende ◽  
Maria Lúcia Carneiro Vieira
Keyword(s):  
Mosaic Virus ◽  
Wild Species ◽  
Virus Disease ◽  
Passion Fruit ◽  
Mechanical Inoculation ◽  
Fruit Crops ◽  
Rt Pcr ◽  
Cowpea Aphid ◽  
Virus Isolates

Cowpea aphid-borne mosaic virus (CABMV) is a potyvirus that causes the most serious virus disease of passion fruit crops in Brazil. It is transmitted by several species of aphids in a non-persistent, non-circulative manner. The reaction of 16 species of Passiflora to infection by mechanical inoculation with four Brazilian isolates of CABMV was evaluated under greenhouse conditions. Only P. suberosa, a wild species, was resistant to infection by all virus isolates, in two independent assays. P. suberosa grafted onto infected P. edulis f. flavicarpa did not develop symptoms; neither was the virus detected by RT-PCR in the upper leaves, suggesting that this species is immune to CABMV.

A fungal glycoprotein mitigates passion fruit woodiness disease caused by Cowpea aphid-borne mosaic virus (CABMV) in Passiflora edulis

BioControl ◽  
2021 ◽  
Author(s):  
José Leonardo Santos-Jiménez ◽  
Caroline de Barros Montebianco ◽  
Andreza Henrique Vidal ◽  
Simone G. Ribeiro ◽  
Eliana Barreto-Bergter ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Passion Fruit ◽  
Passiflora Edulis ◽  
Cowpea Aphid

scholarly journals Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea

2018 ◽  
Vol 40 (1) ◽  
Author(s):  
NAYARA NIELE SACOMAN ◽  
ALEXANDRE PIO VIANA ◽  
VIRGINIA SILVA CARVALHO ◽  
EILEEN AZEVEDO SANTOS ◽  
ROSANA RODRIGUES
Keyword(s):  
Mosaic Virus ◽  
Elisa Test ◽  
Passion Fruit ◽  
The Other ◽  
Visual Evaluation ◽  
Breeding Programs ◽  
Genetic Breeding ◽  
Fruit Species ◽  
Cowpea Aphid

ABSTRACT Passion fruit (Passiflora edulis) is a fruit species of great relevance for Brazilian economy. However, it is highly susceptible to the Cowpea aphid-borne mosaic virus (CABMV). The species P.setacea, on the other hand, is resistant to the disease. The present study aimed to identify CABMV-resistant P. setacea genotypes for the introgression of genes into sour passion fruit genetic breeding programs. The seeds of passion fruit genotypes were germinated in vitro in MS culture medium. The seedlings were acclimatized in a growth chamber at the temperature of 27 ± 2ºC, photoperiod of 16:8 hours and 80% of relative humidity. Thirty plants of each genotype were mechanically inoculated with extract prepared from leaves collected from passion fruit plants with symptoms of CABMV for the assessment of resistance to CABMV. The severity of leaf symptoms was evaluated by means of a grading scale of visual signs. After the visual evaluation and identification of the asymptomatic genotypes of P. setacea, the PTA-ELISA test was carried out for 30 selected genotypes. According to the visual evaluation, all P. setacea genotypes were highly resistant to CABMV, while the P. edulis genotypes were highly susceptible. Out of the 30 genotypes selected, only PsRJ 4 was considered susceptible by PTA-ELISA. The other genotypes of P. setacea were considered resistant and present great potential for use in passion fruit genetic breeding programs.

scholarly journals Yellow passion fruit reaction to Xanthomonas axonopodis pv. passiflorae and to Cowpea aphid-borne mosaic virus

2018 ◽  
Vol 18 (4) ◽  
pp. 349-356
Author(s):  
Anne Pinheiro Costa ◽  
Isadora Nogueira ◽  
José Ricardo Peixoto ◽  
Michelle de Souza Vilela ◽  
Luiz Eduardo Bassay Blum ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Passion Fruit ◽  
Xanthomonas Axonopodis ◽  
Cowpea Aphid ◽  
Yellow Passion Fruit

scholarly journals Transcriptome Sequencing Reveals a Complete Genome Sequence of Cowpea Aphid-Borne Mosaic Virus from Passion Fruit in Kenya

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
F. Munguti ◽  
S. Maina ◽  
E. N. Nyaboga ◽  
D. Kilalo ◽  
E. Kimani ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Transcriptome Sequencing ◽  
Passion Fruit ◽  
Rna Seq ◽  
Cowpea Aphid

Analysis of transcriptome sequencing (RNA-Seq) data revealed a complete Cowpea aphid-borne mosaic virus (CABMV) genome from virus-infected passion fruit in Kenya. We compared it with six complete CABMV genomes, one each from Zimbabwe and Uganda and two each from Brazil and India.

Yellow passion fruit genotypes reaction to woodiness disease (Cowpea aphid-borne mosaic virus) in field conditions

2020 ◽  
pp. 427-432
Author(s):  
G.S. Miranda ◽  
O.K. Yamanishi ◽  
J.R. Peixoto ◽  
M. de S. Vilella ◽  
M. de C. Pires ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Passion Fruit ◽  
Field Conditions ◽  
Cowpea Aphid ◽  
Yellow Passion Fruit

scholarly journals Análise filogenética de potyvírus causando endurecimento dos frutos do maracujazeiro no Nordeste do Brasil

2004 ◽  
Vol 29 (4) ◽  
pp. 378-383 ◽  
Author(s):  
Ana Verônica S. Nascimento ◽  
Ana R. R. Souza ◽  
Poliane F. Alfenas ◽  
Genira P. Andrade ◽  
Murilo G. Carvalho ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Passiflora Edulis ◽  
Cowpea Aphid

Amostras foliares de plantas de maracujá-amarelo (Passiflora edulis f. flavicarpa) com sintomas típicos de endurecimento dos frutos foram coletadas nos estados de Pernambuco, Paraíba e Sergipe. A infecção viral foi comprovada por meio de teste sorológico e gama de hospedeiros. Os seis isolados virais obtidos foram capazes de infetar várias espécies testadas, porém apresentando diferenças na intensidade dos sintomas induzidos nessas hospedeiras. Teste de ELISA indireto demonstrou que os isolados obtidos a partir de plantas de maracujá são sorologicamente relacionados entre si e com o potyvírus Cowpea aphid-borne mosaic virus (CABMV). A seqüência de aminoácidos da proteína capsidial foi determinada para os seis isolados. A comparação dessas seqüências com as de outros potyvírus indicou uma identidade máxima com isolados de CABMV (86 a 94%). A identidade com isolados de Passionfruit woodiness virus (PWV) foi de 68 a 76%. Análise filogenética realizada a partir das seqüências de aminoácidos agrupou os isolados em estudo junto a isolados de CABMV, distante de isolados de PWV. Em conjunto, os resultados indicam que os isolados de maracujá analisados constituem na verdade uma estirpe do CABMV.

Genetic transformation of sweet passion fruit (Passiflora alata) and reactions of the transgenic plants to Cowpea aphid borne mosaic virus

2015 ◽  
Vol 143 (4) ◽  
pp. 813-821 ◽  
Author(s):  
Marcelo Favareto Correa ◽  
Ana Paula Chiaverini Pinto ◽  
Jorge Alberto Marques Rezende ◽  
Ricardo Harakava ◽  
Beatriz Madalena Januzzi Mendes
Keyword(s):  
Transgenic Plants ◽  
Genetic Transformation ◽  
Mosaic Virus ◽  
Passion Fruit ◽  
Cowpea Aphid

scholarly journals Isolation, immunochemical demonstration of field strains of porcine group A rotaviruses and electrophoretic analysis of RNA segments of group A and C rotaviruses

2012 ◽  
Vol 51 (No. 5) ◽  
pp. 288-295 ◽  
Author(s):  
R. Smitalova ◽  
L. Rodak ◽  
I. Psikal ◽  
B. Smid
Keyword(s):  
Cell Line ◽  
Electrophoretic Analysis ◽  
Elisa Test ◽  
Farm Animals ◽  
Economic Losses ◽  
Rt Pcr ◽  
Group C Rotavirus ◽  
Group A Rotaviruses ◽  
Group A ◽  
Virus Isolates

Rotaviruses are major cause of acute diarrhea in animals and humans which can result in huge economic losses in farm animals including pigs. We collected 195 samples of feces of diarrhoeic animals. Rotavirus was demonstrated by electron microscopy using the method of negative staining in 27 samples and by ELISA test using monoclonal antibodies to the group antigen VP6 in 44 samples. Nine samples were selected for virus isolation. Three virus isolates (P375/4, P410/4 and P646/1) were successfully adapted to growth in cell line MA-104. These isolates were allocated to group A rotaviruses based on ELISA, immunoperoxidase test and electropherotype analysis. Electropherotype analysis demonstrated changes during passage in cell line in two of the three isolates. The selected sample P543/1 proved negative in ELISA in a fecal sample. Electropherotype analysis of this sample revealed a “longer” electropherotype profile. The profile was suggestive of group C rotavirus. Rotavirus group C was confirmed by RT-PCR and by sequence analysis in this sample.

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