scholarly journals Synergistic Effects of Supernatants from Serratia marcescens Culture on Larvicidal Activity of Bacillus thuringiensis Cry1C Toxin against Common Cutworm, Spodoptera litura

1999 ◽  
Vol 24 (1) ◽  
pp. 44-48 ◽  
Author(s):  
Shoji ASANO ◽  
Kazushi SUZUKI ◽  
Hidetaka HORI ◽  
Takeshi WATANABE
Keyword(s):  
Bacillus Thuringiensis ◽  
Serratia Marcescens ◽  
Larvicidal Activity ◽  
Spodoptera Litura ◽  
Synergistic Effects ◽  
Common Cutworm

Synergisitic effect of chitinases and Bacillus thuringiensis israelensis spore-toxin complex against Aedes aegypti larvae

2011 ◽  
Vol 143 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Montserrat Ramírez-Suero ◽  
Gerardo Valerio-Alfaro ◽  
Julio S. Bernal ◽  
Mario Ramírez-Lepe
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Serratia Marcescens ◽  
Sole Carbon Source ◽  
Minimal Medium ◽  
Synergistic Effects ◽  
Streptomyces Griseus ◽  
Chitinolytic Activity ◽  
Bacillus Thuringiensis Israelensis ◽  
Toxin Complex

AbstractSix subspecies of Bacillus thuringiensis Berliner (Bt) were grown in minimal medium with chitin as the sole carbon source for 6 days to obtain Bt cell-free fermented broths, which were then evaluated for chitinolytic activity and tested against third-instar Aedes aegypti (L.) (Diptera: Culicidae) larvae. Bt pakistani showed the highest chitinolytic activity (approximately >2700 mU/mL), Bt kurstaki showed the lowest activity (approximately <2000 mU/mL), and Bt thompsoni, Bt aizawai, Bt israelensis, and Bt alesti showed intermediate activities (approximately 2100–2400 mU/mL). Bt aizawai and Bt thompsoni broths showed the highest toxicity (LC50) against third-instar A. aegypti larvae (approximately <290 mU/mL). Bt kurstaki broth showed the lowest toxicity (approximately 420 mU/mL), while Bt pakistani, Bt israelensis, and Bt alesti broths showed intermediate toxicities (approximately 360–460 mU/mL). A purified and biochemically characterized Bt aizawai chitinase and commercial chitinases (from Serratia marcescens Bizio and Streptomyces griseus Waksman and Henrici) were evaluated and compared for synergistic effects on Bt israelensis spore-toxin complex against third-instar A. aegypti larvae. The synergism factor value of Streptomyces griseus and Bt aizawai chitinases were >2 and approximately 1.4; synergism was not evident for the Serratia marcescens chitinase (synergism factor value approximately 0.9).

Chitinolytic activities in Bacillus thuringiensis and their synergistic effects on larvicidal activity

2002 ◽  
Vol 93 (3) ◽  
pp. 374-379 ◽  
Author(s):  
M. Liu ◽  
Q.X. Cai ◽  
H.Z. Liu ◽  
B.H. Zhang ◽  
J.P. Yan ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Larvicidal Activity ◽  
Synergistic Effects

scholarly journals Induction of Antibacterial Activity against Bacillus thuringiensis in the Common Cutworm, Spodoptera litura(Lepidoptera : Noctuidae)

1992 ◽  
Vol 27 (4) ◽  
pp. 565-570 ◽  
Author(s):  
Shyuichiro INAGAKI ◽  
Minoru MIYASONO ◽  
Makiko YAMAMOTO ◽  
Katsuaki OHBA ◽  
Takeo ISHIGURO ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Thuringiensis ◽  
Spodoptera Litura ◽  
Common Cutworm ◽  
The Common ◽  
Lepidoptera Noctuidae
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