scholarly journals Biochemical Changes in Fatty Acids, Hydrocarbons and Sterols as well as Total Lipids of Albino Rats Ingested some Synthetic Colourants and Flavourants as Food Additives

2017 ◽  
Vol 9 (1) ◽  
pp. 40-47
Author(s):  
Hossam S. EL-BELTAGI ◽  
Emam A. ABDEL-RAHIM

The present study was carried out to investigate the effect of ingested synthetic food colourants or flavourants on total lipids content feces. The feces of rats ingested synthetic food colourants or flavourants has more lipids than that of control feces. In treated rat feces some fatty acids were disappeared (C19) or appeared (C14) while the other were increased (C15, C20, C18:2) or decreased (C6) when compared with the control feces. Most of unsaturated fatty acids (un SFA) might bind with the synthetic food colourants or flavourants and secreted in feces, but less consumed than saturated fatty acids (SFA) which, observed on hydrocarbon components, but sterols including cholesterol were more secreted by synthetic food additives treatments. Generally, the increase of total lipids and lipid fractions in feces such as unsaturated fatty acids (un SFA), total sterols (TS) especially cholesterol maybe due to their abilities to bind with food additives and form complex which secreted in feces.

Author(s):  
El-Sayed Ali Abdel-Rahman ◽  
Fawzy A El-Fishawy ◽  
Mohamed A El-Geddawy ◽  
Tomas Kurz ◽  
Mohamed N El-Rify

This study was conducted to assess in detail the possible effects of some technological processes such as soaking, germination, cooking, soaking + cooking, and germination + cooking on the lipid composition of mung bean seeds of Giza 1 variety. TLC analysis of mung bean lipids showed that the phospholipids and triglycerides recorded the highest percentage among lipid fractions (32.26 and 30.10%), while the 1,3 diglycerides constituted the least percentage (2.80%) in mung bean seeds. The soaking, germination and cooking processes caused a decrease in the phospholipids, triglycerides and hydrocarbons accompanied with an increase in monoglycerides, 1,2-(2,3)-diglycerides, sterols and free fatty acids. Eleven fractions were separated from phospholipids class of the studied samples; seven of these fractions were identified. The major component of phospholipids was phosphatidyl choline, amounting to 21.30, 17.84, 16.21, 13.87, 13.20 and 11.47% of the total phospholipids in raw, soaked, germinated, raw-cooked, soaked-cooked and germinated-cooked mung bean seeds, respectively. Gas liquid chromatography of the total lipids of mung bean seeds showed that the unsaturated fatty acids represented 69.58, 64.35, 63.3, 63.16, 61.84 and 61.12%, while the levels of saturated fatty acids were low being 30.37, 34.05, 35.66, 34.64, 37.93 and 38.75% of the total fatty acids in raw, soaked, germinated, raw-cooked, soaked-cooked and germinated-cooked, respectively. The total essential fatty acids (linoleic and linolenic) represented the highest proportion of fatty acids (50.10% of the total fatty acids).


2020 ◽  
Vol 10 (4) ◽  
pp. 665-669
Author(s):  
Lutfi Marzuki ◽  
. Agustono ◽  
Boedi Setya Rahardja

The existence of feed plays an important role in aquaculture activities. This is due to the dominant influence on fish feed growth. Feeding with the right nutritional components can produce healthy and high-quality fish products. One of the nutrients needed by fish is fatty acids. In fact, the provision of fatty acids, one of which is not in the meat, has an important influence. So, this study aims to determine the effect of adding cod liver oil to commercial feed on the ratio of saturated and unsaturated fatty acids to the meat of giant prawn. This research was conducted experimentally with a completely randomized design. The treatment is given a dose of cod liver oil 0% (control), and treatments 1-4 use 3% dose addition to each treatment. On the other hand, in the data analysis stage, the researchers used ANOVA and continued with the Duncan’s test. Based on the results, the study notes that the administration of cod liver oil in commercial feed does not affect the decreasing content of saturated fatty acids in giant prawn meat. On the other hand, the results also showed that the best ratio was found in treatment 4 at a dose of 12%. Therefore, it is concluded that the provision of nutrients for feed related to fatty acids in the pole has not reduced the content of saturated fatty acids. As well as, the best ratio of saturated fatty acids and unsaturated fatty acids was 1.21:1 with cholesterol content of 88.34 mg/dl and this ration resulted by the greatest growth rate in present study.


2017 ◽  
Vol 66 (1) ◽  
pp. 266 ◽  
Author(s):  
Nancy Yazmín Hernández-Nicolás ◽  
Leobigildo Córdova-Téllez ◽  
Angélica Romero-Manzanares ◽  
Jaime Jiménez-Ramírez ◽  
Mario Luna-Cavazos

Jatropha spp. plants are used in traditional medicine, food and for the elaboration of biofuel. The Tehuacán-Cuicatlán Biosphere Reserve in México holds genetic richness and endemism for this genus, but the specific use of the plants and the seed chemical profiles are still unknown. The objectives of this research were to record the traditional forms of use of Jatropha species, and to analyze the chemical composition of Jatropha spp. seeds. For this, a semi-structured survey was conducted in 15 towns, and 20 interviews were applied in each one between May and August 2012. Data allowed to estimate the Significant Use Level and Relative Use Value per species. Besides, seeds from J. neopauciflora, J. rufescens, and J. rzedowskii were collected between August and October 2013, and to seed flour samples we determined total lipids by the Soxhlet method, crude protein by the Kjendahl method, and ashes according to AOAC methods; fatty acid profile and phorbol esters were determined by gas chromatography and by HPLC, respectively. We recorded that the species J. neopauciflora, J. oaxacana, J. rufescens, J. ciliata, and J. rzedowskii, are used as traditional medicine, food, and ornaments, except for J. ciliata, for which none use was recorded. The Significant Use Level as food was found not significant, but for medicinal purposes, J. neopauciflora obtained the highest Relative Use Value (9.0 %). The latex is used to treat 13 disorders, including dental problems (toothache, sensitivity, caries), oral diseases (oral herpes, gingivitis, and oral candidiasis) with a Significant Use Level of 32.9 %; it is also used as a hemostatic. The protein content among species varied from 23.37 to 26.06 %, and total lipids from 34.79 to 36.60 %. The principal unsaturated fatty acids were oleic (25.08 to 30.09 %) and linoleic (44.55 to 48.46 %), and the saturated fatty acids were palmitic (10.11 to 16.50 %) and stearic (9.47 to 11.15 %). Phorbol esters, the main cause of seed’s toxicity, were absent in J. neopauciflora, J. rufescens, and J. rzedowskii. In conclusion, the Significant Use Level of Jatropha species studied was low, with little cultural acceptance and sporadic utilization. The Relative Use Value was important for medicinal purposes, especially for J. neopauciflora. The dehulled seeds of J. neopauciflora, J. rufescens, and J. rzedowskii are potentially useful as food, having high protein contents, and unsaturated oleic and linoleic fatty acids. With this study we report three non-toxic Jatropha species and recommend to scientifically validate the antimycotic use of J. neopauciflora latex.


Author(s):  
Abdulrahman L. Al-Malki ◽  
Elie K. Barbour ◽  
Khadijah S. Balamash ◽  
Fawzia A. Alshubaily ◽  
Khalid O. Abualnaja ◽  
...  

Aim: This study evaluated the effect of Phyocyanin extracted from Chlorophyta Ulva lactuca algae loaded on albumin nano-particles (ULANP) on diabetic rats. Materials and Methods: Fifty albino rats were divided into 5 goups.  GPI: control and GPII: rats were injected with alloxan (75 mg /kg) i.p for six consecutive days for induction of diabetes. This group was subdivided into 4 subgroups: GP IIa: (Untreated diabetic): GP IIb: rats were given with ULANP (100 mg/kg).GP IIc: Rats were given ULANP (200 mg/kg) i.p. GP IId: Rats were given insulin (100 unit/ day). Serum NO, interleukin-6 glucose, AGEs and fatty acids profile was determined. Results:  Analysis of ULANP by FTIR showed the characteristic band (2100 cm-1~ 3700 cm-1) that is indicated mainly from -COO, – CO and conjugated double bond. These bonds showed spectral bands peak 2985 cm-1 and 2860 cm-1, 2986 cm-1.  Administration of ULANP in diabetic rats exerted an anti-inflammatory by lowering NO and IL-6 levels and hypoglycemic effects by decreased glucose and reduced AGEs levels. In addition, ULAPN lowered percent of saturated fatty acids while elevated unsaturated fatty acids percent. Conclusion: It was concluded that, ULAPN is a promising effective anti-inflammatory and hypoglycemic agent compared with other therapeutic agents with lower site effects. 


2004 ◽  
Vol 59 (5-6) ◽  
pp. 330-334 ◽  
Author(s):  
Fotini Malisiova ◽  
Sophia Hatziantoniou ◽  
Kostas Dimas ◽  
Dimitrios Kletstas ◽  
Costas Demetzos

The seeds of the almond tree [(Prunus dulcis (Mill.) D. A. Webb. (syn. Prunus amygdalus)] were collected in two different periods of maturity and were studied for their lipid content. The total lipids (TL) were extracted by the Bligh-Dyer method and the lipid classes have been isolated by chromatographic techniques and were analyzed by HPTLC coupled with a flame ionization detector (HPTLC/FID) and GC-MS. The oils were found to be rich in neutral lipids (89.9% and 96.3% of total lipids) and low in polar lipids (10.1% and 3.7% of total lipids) for the immature and mature seed oils, respectively. The neutral lipid fraction consisted mainly of triacylglycerides whereas the polar lipids mainly consisted of phospholipids. GC-MS data showed that the main fatty acid for both oils was 9-octadecenoic acid (oleic acid). The unsaturated fatty acids were found as high as 89.4% and 89.7%, while the percentage of the saturated fatty acids was found 10.6% and 10.3% for the immature and mature seed oils, respectively. Liposomes were prepared from the isolated phospholipids using the thin lipid film methodology, and their physical properties were characterized. Cytotoxicity was found absent when assayed against normal and cancerous cell lines. These new formulations may have future applications for encapsulation and delivery of drugs and cosmetically active ingredients.


1958 ◽  
Vol 36 (1) ◽  
pp. 653-657 ◽  
Author(s):  
T. K. Murray ◽  
J. L. Beare ◽  
J. A. Campbell ◽  
C. Y. Hopkins

Fat mixtures containing 10, 30, 60, and 70% saturated fatty acids as glycerides were fed to male rats at a level of 17% by weight of a chow diet. The saturated acids were mainly palmitic rather than lauric and myristic as previously used. In confirmation of earlier results the fat mixture containing 30% saturated fatty acids produced the greatest weight gains and the highest food efficiency. The same trend was noted when similar fat mixtures were added to a purified diet but differences in weight gain and food efficiency were not significant. The source of fatty acids had no effect on weight gains. The excretion of total lipids was not influenced by the degree of saturation of the fats within the limits studied. Shortening, however, caused increased excretion of total lipid. There were no significant differences in liver and adrenal weights produced by these diets.


2013 ◽  
Vol 448-453 ◽  
pp. 1079-1084 ◽  
Author(s):  
Pu Gong ◽  
Gan Ran Deng ◽  
Jian Hua Cao ◽  
Guo Jie Li ◽  
Zhi Liu ◽  
...  

Crude palm oil (CPO) was extracted from fresh fruit bunches of RYL7 oil palm cultivated in Hainan by using a self-made single stage screw press. The physicochemical characteristics and Fatty acid composition of the CPO was investigated. The experimental results included melting point (33.10 °C), density (0.91 g/cm3 at 20 °C), acid value (8.35 mg KOH/g), iodine value (62.72 mg iodine/g), saponifiable value (198.02 mg KOH/g), moisture and volatile matter (0.16% of total lipids), insoluble impurities (0.04% of total lipids), unsaponifiable matter (0.40% of total lipids). Oleic acid (40.90% of total fatty acids), palmitic acid (37.88% of total fatty acids), linoleic (14.29% of total fatty acids), followed by stearic acid (5.11% of total fatty acids) were found to be the predominant fatty acids in the oil. The unsaturated oleic acid was the most predominant fatty acid in CPO of Hainan while saturated palmitic acid was the most principal fatty acid in palm oil from Malaysia. The contents of linolenic, unsaturated fatty acids, and polyunsaturated fatty acids in this CPO were 4.09%, 5.09%, 4.09% higher than that of Malaysia, respectively. In addition, the percentages of palmitic acid and saturated fatty acids of this oil were 5.62%, 6.01% lower than that of Malaysia, respectively.


1958 ◽  
Vol 36 (7) ◽  
pp. 653-657 ◽  
Author(s):  
T. K. Murray ◽  
J. L. Beare ◽  
J. A. Campbell ◽  
C. Y. Hopkins

Fat mixtures containing 10, 30, 60, and 70% saturated fatty acids as glycerides were fed to male rats at a level of 17% by weight of a chow diet. The saturated acids were mainly palmitic rather than lauric and myristic as previously used. In confirmation of earlier results the fat mixture containing 30% saturated fatty acids produced the greatest weight gains and the highest food efficiency. The same trend was noted when similar fat mixtures were added to a purified diet but differences in weight gain and food efficiency were not significant. The source of fatty acids had no effect on weight gains. The excretion of total lipids was not influenced by the degree of saturation of the fats within the limits studied. Shortening, however, caused increased excretion of total lipid. There were no significant differences in liver and adrenal weights produced by these diets.


2000 ◽  
Vol 55 (3-4) ◽  
pp. 245-255 ◽  
Author(s):  
Vassilia J. Sinanoglou ◽  
Sofia Miniadis-Meimaroglou

Abstract Polar lipids of the cephalopods Eledone moschata, Sepia officinalis and Todarodes sagittatus mantle, represent 50.5%, 66.1% and 74.2% of wet tissue respectively. On the other hand the polar lipids of these three species of cephalopods constitute of 80.8%, 94.8% and 93.7% of phospholipids, respectively. The main phospholipids identified were phosphatidylcholine (52.2, 51.3 and 58.4% of total phospholipids respectively in the above mentioned species), phosphatidylethanolamine (18.1, 19.7 and 23.9%), sphingomyelin (10.7, 15.2 and 6.7%), lysophosphatidylcholine (3.1, 3.8 and 1.8%) and the unusual lipid ceramide aminoethylphosphonic acid (15.9, 10 and 9.2%). The 56.8% of phosphatidylcholine in Eledone moschata, the 46% in Sepia officinalis and the 74.1% in Todarodes sagittatus refer to the structure of 1,2-diacyl-glycerocholine and the remaining percentage refer to the structure of 1-o-alkyl-2-acyl-glycerocholine or 1-o-alkyl-lenyl- 2-acyl-glycerocholine. The 87.2% of phosphatidylethanolamine in Eledone moschata, the 81% in Sepia officinalis and the 90.7% in Todarodes sagittatus refer to the structure of 1,2-diacyl-glyceroethanolamine and the remaining percentage refer to the structure of 1-o-alkyl-2-acyl-glyceroethanolamine or 1-o-alkyl-1-enyl-2-acyl-glyceroethanolamine. The major saturated fatty acids in phosphatidylcholine and phosphatidylethanolamine were C16:0 (30.3-67.5% and 23.2-54.5% ) and C18:0 (3.6-17% and 15.4-28% ), respectively, while the major unsaturated fatty acids in these lipids were C18:1n-9, n-7 (1.0-7.3% and 5.3-10.5% ), C20:5n-3 (1.5-9.8% and 4,5-15.8% ) and C22:6n-3 (12.5-42.0% and 7 .0 - 11.3%), respectively.


Author(s):  
Olawale Paul Olatidoye ◽  
Taofik Akinyemi Shittu ◽  
Samuel Olusegun Awonorin ◽  
Emmanuel Sunday Ajisegiri

This study aimed at evaluating the effect of roasting conditions on some physicochemical and nutritional properties of cashew nut lipid fractions at different temperature (100–160ᵒC) and time (20–60 min). Raw and roasted cashew nuts were also analyzed for their composition in fatty acid by GC-FID. Physicochemical properties of the oil showed that the oil yield of roasted cashew nut oil (56.67-31.67%) was significantly higher compared to unroasted oil (47.43%). Also, cashew nut oil with mean values revealed that acid (12-0.86 mg KOH/g of oil); saponification value (138.10-169.06 mgKOH/g of oil), iodine value (55.50-36.19 mg of I/100g of oil); TBA(0.012-0.18mg); p-anisidine value (0.55-0.66); peroxide value (7.26-3.05 meq/kg) and free fatty acid (1.07-1.50 % oleic acid) which indicated that cashew nut oil is edible, non-drying and may not be suitable for soap making. Results of GC-FID analysis of the ethyl esters of saturated and unsaturated fatty acid of raw and roasted cashew nut showed that unsaturated fatty acids represented 60.57% for unroasted samples with roasted samples (79.67–83.67%) while saturated fatty acids recorded (16.11–37.65%). The fatty acid composition of the oils shows the presence of four main fatty acids: palmitic (9.25–29.50%); stearic (4.4–9.34%); oleic (34.75–65.60%); and linoleic (1.35–20.66%) with high oleic to linoleic ratio associated with high oil stability. The order of increase is oleic > linoleic > palmitic > stearic in all the samples irrespective of roasting conditions. The result showed that minor changes occur in the fatty acids composition as the roasting temperature and time increased. It was concluded that the heat treatment used does not significantly affect the fatty acid profile content of cashew nuts preserving its antioxidant activity along with other bioactive compounds contained therein.


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