FURTHER STUDIES ON THE OPTIMUM RATIO OF SATURATED TO MONO-UNSATURATED FATTY ACIDS IN RAT DIETS

1958 ◽  
Vol 36 (7) ◽  
pp. 653-657 ◽  
Author(s):  
T. K. Murray ◽  
J. L. Beare ◽  
J. A. Campbell ◽  
C. Y. Hopkins

Fat mixtures containing 10, 30, 60, and 70% saturated fatty acids as glycerides were fed to male rats at a level of 17% by weight of a chow diet. The saturated acids were mainly palmitic rather than lauric and myristic as previously used. In confirmation of earlier results the fat mixture containing 30% saturated fatty acids produced the greatest weight gains and the highest food efficiency. The same trend was noted when similar fat mixtures were added to a purified diet but differences in weight gain and food efficiency were not significant. The source of fatty acids had no effect on weight gains. The excretion of total lipids was not influenced by the degree of saturation of the fats within the limits studied. Shortening, however, caused increased excretion of total lipid. There were no significant differences in liver and adrenal weights produced by these diets.

1958 ◽  
Vol 36 (1) ◽  
pp. 653-657 ◽  
Author(s):  
T. K. Murray ◽  
J. L. Beare ◽  
J. A. Campbell ◽  
C. Y. Hopkins

Fat mixtures containing 10, 30, 60, and 70% saturated fatty acids as glycerides were fed to male rats at a level of 17% by weight of a chow diet. The saturated acids were mainly palmitic rather than lauric and myristic as previously used. In confirmation of earlier results the fat mixture containing 30% saturated fatty acids produced the greatest weight gains and the highest food efficiency. The same trend was noted when similar fat mixtures were added to a purified diet but differences in weight gain and food efficiency were not significant. The source of fatty acids had no effect on weight gains. The excretion of total lipids was not influenced by the degree of saturation of the fats within the limits studied. Shortening, however, caused increased excretion of total lipid. There were no significant differences in liver and adrenal weights produced by these diets.


1955 ◽  
Vol 33 (1) ◽  
pp. 1047-1054 ◽  
Author(s):  
C. Y. Hopkins ◽  
T. K. Murray ◽  
J. A. Campbell

Fat mixtures were prepared in which the content of linoleic acid was held constant at approximately 10% of the total fatty acids, but the ratio of saturated to mono-unsaturated acids was varied from 3.5: 1 to 1: 8. These mixtures were added to a low-fat fox chow diet at the rate of 20 parts fat to 80 parts fox chow and the resulting mixtures were fed to weanling rats in one experiment and to semiadult rats in another. The feeding period was nine weeks. In both experiments the male rats showed significant differences in final weight between the diets. Best weight gains were made on the fat mixture in which the fatty acid ratio was close to that of normal rat depot fat, viz. one part of saturated acid to two parts of mono-unsaturated acid. Female rats did not show significant differences in weight gain on the various fat mixtures. Excretion of lipid in the feces showed a tendency to increase with increasing ratios of unsaturated to saturated fatty acids in the diet.


1955 ◽  
Vol 33 (6) ◽  
pp. 1047-1054 ◽  
Author(s):  
C. Y. Hopkins ◽  
T. K. Murray ◽  
J. A. Campbell

Fat mixtures were prepared in which the content of linoleic acid was held constant at approximately 10% of the total fatty acids, but the ratio of saturated to mono-unsaturated acids was varied from 3.5: 1 to 1: 8. These mixtures were added to a low-fat fox chow diet at the rate of 20 parts fat to 80 parts fox chow and the resulting mixtures were fed to weanling rats in one experiment and to semiadult rats in another. The feeding period was nine weeks. In both experiments the male rats showed significant differences in final weight between the diets. Best weight gains were made on the fat mixture in which the fatty acid ratio was close to that of normal rat depot fat, viz. one part of saturated acid to two parts of mono-unsaturated acid. Female rats did not show significant differences in weight gain on the various fat mixtures. Excretion of lipid in the feces showed a tendency to increase with increasing ratios of unsaturated to saturated fatty acids in the diet.


1963 ◽  
Vol 41 (1) ◽  
pp. 605-612 ◽  
Author(s):  
Joyce L. Beare ◽  
J. A. Campbell ◽  
C. G. Youngs ◽  
B. M. Craig

The effects of increasing the saturated fatty acids in a dietary vegetable oil composed mostly of unsaturated fatty acids were studied in rats. A mixture of palm oil and Swedish rapeseed oil fed for 4 weeks as 20% of a purified diet promoted weight gains which exceeded those obtained with Polish rapeseed oil of a similar content of erucic acid, and altered the proportion of saturated fatty acids in the tissues to reflect that of the diet. When methyl esters of saturated fatty acids were added to Swedish rapeseed oil, similar effects on weight gain were not observed, but methyl esters of fatty acids from corn oil and rapeseed oil were shown to be of less nutritional value than the original glycerides. From fatty acids of olive oil, glycerides containing 3% palmitic acid were prepared, and produced weight gains which did not differ significantly from those of rats fed Polish rapeseed oil with a similar content of palmitic acid and 20% erucic acid. The characteristic effects of rapeseed oil are, therefore, attributed to its low content of saturated fatty acids as well as its high content of erucic acid.


1963 ◽  
Vol 41 (3) ◽  
pp. 605-612 ◽  
Author(s):  
Joyce L. Beare ◽  
J. A. Campbell ◽  
C. G. Youngs ◽  
B. M. Craig

The effects of increasing the saturated fatty acids in a dietary vegetable oil composed mostly of unsaturated fatty acids were studied in rats. A mixture of palm oil and Swedish rapeseed oil fed for 4 weeks as 20% of a purified diet promoted weight gains which exceeded those obtained with Polish rapeseed oil of a similar content of erucic acid, and altered the proportion of saturated fatty acids in the tissues to reflect that of the diet. When methyl esters of saturated fatty acids were added to Swedish rapeseed oil, similar effects on weight gain were not observed, but methyl esters of fatty acids from corn oil and rapeseed oil were shown to be of less nutritional value than the original glycerides. From fatty acids of olive oil, glycerides containing 3% palmitic acid were prepared, and produced weight gains which did not differ significantly from those of rats fed Polish rapeseed oil with a similar content of palmitic acid and 20% erucic acid. The characteristic effects of rapeseed oil are, therefore, attributed to its low content of saturated fatty acids as well as its high content of erucic acid.


1958 ◽  
Vol 36 (5) ◽  
pp. 629-632 ◽  
Author(s):  
H. L. House ◽  
D. F. Riordan ◽  
J. S. Barlow

The resistance of larvae of Pseudosarcophaga affinis (Fall.) to a high temperature was increased by thermal conditioning and by dietary lipids. The time of exposure to 45 °C required to cause 50% mortality of larvae reared at 23 °C on pork liver was 130 minutes (5% confidence limits 127 and 132 minutes). Pre-conditioning for 2 hours at 39 °C increased this time to 200 minutes (183 and 218 minutes). The time of exposure to 45 °C required to cause 50% mortality of larvae reared on chemically defined diets was 177 minutes (159 and 196 minutes) for those on a fat mixture that contained unsaturated fatty acids, 184 minutes (164 and 206 minutes) for those reared on an intermediate mixture, and 218 minutes (198 and 240 minutes) for those reared on a diet that contained a high proportion of saturated fatty acids. The degree of saturation of body lipids was directly influenced by the degree of saturation of the dietary lipids.


2017 ◽  
Vol 66 (1) ◽  
pp. 266 ◽  
Author(s):  
Nancy Yazmín Hernández-Nicolás ◽  
Leobigildo Córdova-Téllez ◽  
Angélica Romero-Manzanares ◽  
Jaime Jiménez-Ramírez ◽  
Mario Luna-Cavazos

Jatropha spp. plants are used in traditional medicine, food and for the elaboration of biofuel. The Tehuacán-Cuicatlán Biosphere Reserve in México holds genetic richness and endemism for this genus, but the specific use of the plants and the seed chemical profiles are still unknown. The objectives of this research were to record the traditional forms of use of Jatropha species, and to analyze the chemical composition of Jatropha spp. seeds. For this, a semi-structured survey was conducted in 15 towns, and 20 interviews were applied in each one between May and August 2012. Data allowed to estimate the Significant Use Level and Relative Use Value per species. Besides, seeds from J. neopauciflora, J. rufescens, and J. rzedowskii were collected between August and October 2013, and to seed flour samples we determined total lipids by the Soxhlet method, crude protein by the Kjendahl method, and ashes according to AOAC methods; fatty acid profile and phorbol esters were determined by gas chromatography and by HPLC, respectively. We recorded that the species J. neopauciflora, J. oaxacana, J. rufescens, J. ciliata, and J. rzedowskii, are used as traditional medicine, food, and ornaments, except for J. ciliata, for which none use was recorded. The Significant Use Level as food was found not significant, but for medicinal purposes, J. neopauciflora obtained the highest Relative Use Value (9.0 %). The latex is used to treat 13 disorders, including dental problems (toothache, sensitivity, caries), oral diseases (oral herpes, gingivitis, and oral candidiasis) with a Significant Use Level of 32.9 %; it is also used as a hemostatic. The protein content among species varied from 23.37 to 26.06 %, and total lipids from 34.79 to 36.60 %. The principal unsaturated fatty acids were oleic (25.08 to 30.09 %) and linoleic (44.55 to 48.46 %), and the saturated fatty acids were palmitic (10.11 to 16.50 %) and stearic (9.47 to 11.15 %). Phorbol esters, the main cause of seed’s toxicity, were absent in J. neopauciflora, J. rufescens, and J. rzedowskii. In conclusion, the Significant Use Level of Jatropha species studied was low, with little cultural acceptance and sporadic utilization. The Relative Use Value was important for medicinal purposes, especially for J. neopauciflora. The dehulled seeds of J. neopauciflora, J. rufescens, and J. rzedowskii are potentially useful as food, having high protein contents, and unsaturated oleic and linoleic fatty acids. With this study we report three non-toxic Jatropha species and recommend to scientifically validate the antimycotic use of J. neopauciflora latex.


2004 ◽  
Vol 59 (5-6) ◽  
pp. 330-334 ◽  
Author(s):  
Fotini Malisiova ◽  
Sophia Hatziantoniou ◽  
Kostas Dimas ◽  
Dimitrios Kletstas ◽  
Costas Demetzos

The seeds of the almond tree [(Prunus dulcis (Mill.) D. A. Webb. (syn. Prunus amygdalus)] were collected in two different periods of maturity and were studied for their lipid content. The total lipids (TL) were extracted by the Bligh-Dyer method and the lipid classes have been isolated by chromatographic techniques and were analyzed by HPTLC coupled with a flame ionization detector (HPTLC/FID) and GC-MS. The oils were found to be rich in neutral lipids (89.9% and 96.3% of total lipids) and low in polar lipids (10.1% and 3.7% of total lipids) for the immature and mature seed oils, respectively. The neutral lipid fraction consisted mainly of triacylglycerides whereas the polar lipids mainly consisted of phospholipids. GC-MS data showed that the main fatty acid for both oils was 9-octadecenoic acid (oleic acid). The unsaturated fatty acids were found as high as 89.4% and 89.7%, while the percentage of the saturated fatty acids was found 10.6% and 10.3% for the immature and mature seed oils, respectively. Liposomes were prepared from the isolated phospholipids using the thin lipid film methodology, and their physical properties were characterized. Cytotoxicity was found absent when assayed against normal and cancerous cell lines. These new formulations may have future applications for encapsulation and delivery of drugs and cosmetically active ingredients.


2013 ◽  
Vol 448-453 ◽  
pp. 1079-1084 ◽  
Author(s):  
Pu Gong ◽  
Gan Ran Deng ◽  
Jian Hua Cao ◽  
Guo Jie Li ◽  
Zhi Liu ◽  
...  

Crude palm oil (CPO) was extracted from fresh fruit bunches of RYL7 oil palm cultivated in Hainan by using a self-made single stage screw press. The physicochemical characteristics and Fatty acid composition of the CPO was investigated. The experimental results included melting point (33.10 °C), density (0.91 g/cm3 at 20 °C), acid value (8.35 mg KOH/g), iodine value (62.72 mg iodine/g), saponifiable value (198.02 mg KOH/g), moisture and volatile matter (0.16% of total lipids), insoluble impurities (0.04% of total lipids), unsaponifiable matter (0.40% of total lipids). Oleic acid (40.90% of total fatty acids), palmitic acid (37.88% of total fatty acids), linoleic (14.29% of total fatty acids), followed by stearic acid (5.11% of total fatty acids) were found to be the predominant fatty acids in the oil. The unsaturated oleic acid was the most predominant fatty acid in CPO of Hainan while saturated palmitic acid was the most principal fatty acid in palm oil from Malaysia. The contents of linolenic, unsaturated fatty acids, and polyunsaturated fatty acids in this CPO were 4.09%, 5.09%, 4.09% higher than that of Malaysia, respectively. In addition, the percentages of palmitic acid and saturated fatty acids of this oil were 5.62%, 6.01% lower than that of Malaysia, respectively.


2017 ◽  
Vol 9 (1) ◽  
pp. 40-47
Author(s):  
Hossam S. EL-BELTAGI ◽  
Emam A. ABDEL-RAHIM

The present study was carried out to investigate the effect of ingested synthetic food colourants or flavourants on total lipids content feces. The feces of rats ingested synthetic food colourants or flavourants has more lipids than that of control feces. In treated rat feces some fatty acids were disappeared (C19) or appeared (C14) while the other were increased (C15, C20, C18:2) or decreased (C6) when compared with the control feces. Most of unsaturated fatty acids (un SFA) might bind with the synthetic food colourants or flavourants and secreted in feces, but less consumed than saturated fatty acids (SFA) which, observed on hydrocarbon components, but sterols including cholesterol were more secreted by synthetic food additives treatments. Generally, the increase of total lipids and lipid fractions in feces such as unsaturated fatty acids (un SFA), total sterols (TS) especially cholesterol maybe due to their abilities to bind with food additives and form complex which secreted in feces.


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