scholarly journals Studies on Hormonal Effects on Rooting of Marcotting and Stem-Cuttings of Akee Apple (Blighia sapida K. D. Koenig)

2016 ◽  
Vol 8 (4) ◽  
pp. 468-471 ◽  
Author(s):  
Ehoniyotan Olayemi IBUKUN
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Callus Formation ◽  
Hormonal Treatment ◽  
The Other ◽  
Naphthalene Acetic Acid ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Hormonal Treatments

The effect of hormone on the rooting of stem-cuttings and marcotting of akee apple was studied using a combination between Indole -3- Butyric Acid (IBA) and 1- Naphthalene Acetic Acid (NAA). Stem-cuttings from mature akee trees from Challenge, Jalala and Ganmo in Ilorin, were treated with different dilutions of the liquid hormone in the combination of 1.0% Indole-3- butyric acid + 0.5% 1-Naphthaleneacetic acid before propagating them in a non-mist propagator. Marcotting was also carried-out on trees, using the hormonal combination of different dilutions. Observations and the results obtained revealed that the hormonal combination had significant effect on the rooting of stem-cuttings and marcotting. Both marcotting and stem cuttings did not produce at the end of the experiment roots in the absence of the hormonal treatment; a particular aspect was marcotting that initials produced roots. On the other hand, both marcotting and stem cuttings produced roots with the hormonal treatments; more roots were produced using the combination of 2,000 ppm of IBA and 1,000 ppm of NAA, compared with lower concentrations of the hormone mixtures. After callus formation, 2,000 ppm of IBA and 1,000 ppm of NAA combination gave the best results within stem-cuttings. Based on the results obtained, it was concluded that the combination of IBA and NAA in appropriate concentration promoted rooting in Akee apple and therefore are highly valuable for the vegetative propagation of this species through stem cutting and marcotting.

scholarly journals Impact of Auxins on Vegetative Propagation through Stem Cuttings of Couroupita guianensis Aubl.: A Conservation Approach

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Acetic Acid ◽  
Vegetative Propagation ◽  
Adventitious Shoots ◽  
Conservation Strategy ◽  
Soil Conditions ◽  
Natural Soil ◽  
Naphthalene Acetic Acid ◽  
Stem Cuttings ◽  
Medicinal Tree ◽  
Couroupita Guianensis

The present study explores the potential of exogenous auxins in the development of adventitious shoots and roots from shoot cuttings of Couroupita guianensis (Nagalingam), a threatened tree. Experiments were conducted to assess the effect of various concentrations of auxins on shoot and root morphological traits of stem cuttings in the greenhouse. Amongst the auxins tested, significant effects on number of shoot buds’ induction and their growth were observed with α-Naphthalene Acetic Acid (NAA) treated nodal cuttings. Cent percentage of the stem cuttings of C. guianensis were rooted and shoots were induced when pretreated with 400 mg L−1 NAA for 5 min. Maximum 79% of stem cuttings responded to pretreatment of 300 mg L−1 indole-3-butyric acid (IBA) for 5 min, and 75% of stem cuttings induced shoots with 400 mg L−1 indole-3-acetic acid (IAA). Presence of at least 5 nodes on stem cuttings was found to be prerequisite for root and shoot induction. About 92% of plants were survived under natural soil conditions raised from the stem cuttings. This is the first report of vegetative propagation of C. guianensis through stem cuttings which could be used for conservation strategy and sustainable utilization of this threatened medicinal tree.

scholarly journals Vegetative propagation of Garcinia lucida Vesque (Clusiaceae) using leafy stem cuttings and grafting

Afrika Focus ◽  
2017 ◽  
Vol 27 (3) ◽  
Author(s):  
Bertin Takoutsing ◽  
Alain Tsobeng ◽  
Zacharie Tchoundjeu ◽  
Ann Degrande ◽  
Ebenazar Asaah
Keyword(s):  
Acetic Acid ◽  
Leaf Area ◽  
Vegetative Propagation ◽  
Naphthalene Acetic Acid ◽  
High Porosity ◽  
Stem Cuttings ◽  
Forest Zone ◽  
Indole Butyric Acid ◽  
Humid Forest ◽  
Humid Forest Zone

Garcinia lucida Vesque (Clusiaceae) is a tree species that is highly valued for its medicinal properties by rural households in the humid forest zone of Cameroon. However, the unsustainable exploitation of the species threatens its long-term regeneration. This study focuses on its vegetative propagation via stem cuttings in non-mist propagators and through grafting. The study tests the effects of three rooting media (sand, sawdust, sand + sawdust (1/1); three leaf sizes (0,25 and 50 cm2); and three types of hormone [indole butyric acid (IBA), indole-3-acetic acid (IAA) and naphthalene acetic acid (NAA)], applied as a single dose. Furthermore, three grafting techniques (cleft, side tongue and whip-and-tongue grafting) were tested. All experiments were designed as completely randomized blocks with three replicates. Results showed that cuttings require a medium that has low water holding capacity and high porosity, and a leaf area of 50 cm2 and NAA treatment; grafting success was affected by the technique used, with top cleft grafting yielding a 100% success rate. From this preliminary study, it is concluded that G. lucida is amenable to vegetative propagation by cuttings and grafting. Key words: tree domestication, leaf area, non-mist propagator, rooting hormone, rooting medium, under-utilised species 

scholarly journals Rooting Efficiency of Amur Maple Seedless Selections Produced by Mutagenesis1

2020 ◽  
Vol 38 (2) ◽  
pp. 37-43
Author(s):  
Andrzej K. Noyszewski ◽  
Alan G. Smith
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Adventitious Rooting ◽  
Naphthalene Acetic Acid ◽  
Stem Cuttings ◽  
Customer Choice ◽  
Asexual Propagation ◽  
Propagation Methods ◽  
Noxious Weed ◽  
Acer Ginnala

Abstract Amur maple (Acer ginnala Maxim.) is a widely planted small tree with attractive red fall foliage. In many states, it is classified as a noxious weed and regulations restrict propagation and sale, limiting customer choice. Stem cuttings are commonly used as a means of asexual propagation of Amur maple, with softwood cuttings being the easiest to root. Here we report adventitious rooting efficiency of seedless Amur maple selections using four indole-3-butyric acid (IBA; 500, 1,500, 5,000, 10,000 ppm) and four naphthalene acetic acid (NAA; 100, 500, 1,500, 5,000 ppm) treatments and a no hormone control. Overall, cuttings treated with 5,000 or 10,000 ppm IBA and 5,000 ppm NAA resulted in the highest percent rooting (PR), mean number of roots (MNR), and mean length of the longest root (MRL) across all seedless selections. Seedless selections SW-30-14 and SW-30-130 had the highest PR, 66% and 67%, respectively, and will perform well in commercial propagation. This level of rooting was greater than that of the cultivar ‘Bailey Compact' that had 46% PR, and the seedless selection SW-30-159 that had the lowest PR (10.3%), MNR (0.3), and MRL (0.2 in) averaged across all treatments. Cuttings from 4 of the 5 seedless selections treated with 1,500 ppm NAA had the greatest PR, whereas ‘Bailey Compact' and SW-30-159 had the greatest PR with 5,000 ppm NAA. Results indicate that rooting efficiency was impacted by mutagenesis in some selections, which may require optimization of propagation methods for those selections. Index words: adventitious rooting, auxin, vegetative propagation, sterile, non-native invasive. Species used in this study: Amur maple (Acer ginnala Maxim.). Chemicals used in this study: indole-3-butyric acid (IBA; 500, 1,500, 5,000, 10,000 ppm) and naphthalene acetic acid (NAA; 100 500, 1,500 5,000 ppm).

scholarly journals Cutting Type and Auxin Treatment Affect Rooting of Cupressus cashmeriana

2012 ◽  
Vol 30 (4) ◽  
pp. 211-213
Author(s):  
Annie Raup ◽  
Matthew D. Taylor
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Plant Material ◽  
Potassium Salt ◽  
Adventitious Rooting ◽  
Naphthalene Acetic Acid ◽  
Auxin Concentration ◽  
Evergreen Conifer ◽  
Significant Factors

Cupressus cashmeriana is an ornamental evergreen conifer with little published information on vegetative propagation. Two experiments were conducted to determine the effects of cutting type and auxin concentration and form on adventitious rooting. Experiment 1 evaluated three cutting treatments (softwood, hardwood, and mallet) and four auxin treatments consisting of 0, 2500, 5000, and 10000 ppm potassium salt of indole-3-butyric acid (K-IBA). Experiment 2 evaluated the same cutting types as experiment 1 and auxin treatments consisted of 5000 ppm K-IBA, 5000 ppm naphthalene acetic acid (NAA), 2500 ppm K-IBA in combination with 2500 ppm NAA and zero auxin applied. In both experiments, rooting of softwood and hardwood cuttings was significantly higher than mallet cuttings. Treatment of cuttings with different rates and types of auxin lead to inconclusive results, which indicate auxin may not be beneficial for rooting of C. cashmeriana. This research suggests that the age of plant material is one of the most significant factors for successful rooting C. cashmeriana.

scholarly journals Effects of Variations in Hormonal Treatments Upon In vitro Regeneration Potential in Embryo Explants of Arenga pinnata

2021 ◽  
Vol 17 (5) ◽  
pp. 495-503
Author(s):  
Shamsiah Abdullah ◽  
Siti Nurain Roslan
Keyword(s):  
Acetic Acid ◽  
In Vitro Regeneration ◽  
Hormonal Treatment ◽  
Height Increment ◽  
Regeneration Potential ◽  
In Vitro Method ◽  
Indole 3 Acetic Acid ◽  
Hormonal Treatments

One of the challenges related to propagation of Arenga pinnata is its lengthy period of seed dormancy. In this study, in vitro regeneration was carried out to determine the effect of hormonal treatment on the embryo explant of Arenga pinnata. Embryos were surface sterilized and cultured into different media supplemented with various hormones concentrations and combinations. Each treatment contained of Kinetin (KN) hormone (1.0, 2.0, and 3.0 mg/l) and in combination with indole-3-acetic acid (IAA) of 0.1, 0.2, 0.3 mg/l. The height of plumule and length of radical was observed and recorded. Treatment 8 (3 mg/ml KN + 0.1 mg/ml IAA) showed 59.09% in plumule height increment while treatment 4 (1 mg/ml KN + 0.3 mg/ml IAA) showed the highest radical increments with 93.62%. The knowledge gained in this study consequently helps us to better understand the role of KN and IAA in the in vitro regeneration protocol. Since in vitro method able to produce higher number of in vitro seedlings at one time, it is important to establish the in vitro regeneration protocol for this plant.

scholarly journals Propagation of three native Brazilian Piper species by cuttings and indole butyric acid

2018 ◽  
Vol 12 (2) ◽  
pp. 491-499
Author(s):  
Aurea Portes Ferriani ◽  
Cícero Deschamps ◽  
Wanderlei Do Amaral ◽  
Luiz Everson da Silva
Keyword(s):  
New Species ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Biological Activities ◽  
Average Diameter ◽  
Adventitious Rooting ◽  
Stem Cuttings ◽  
Indole Butyric Acid ◽  
Positive Effect ◽  
Biological Reserve

Native Piper species present significant repellent, antimicrobial, inseticidal, anti-tumor and anti-protozoal biological activities. Studies on new species can discover unpublished potentialities and vegetative propagation for the development of cultivation protocols and reduce the natural extraction. The objective of this study was to evaluate the rooting of P. arboreum, P. cernuum and P. diospyrifolium stem cuttings with different treatments containing doses of indole butyric acid (0, 500; 1,000; 1,500; 2,000 and 3,000 mg L-1). Branches were collected in the “Bom Jesus Biological Reserve”, Parana state, Brazil. The cuttings, with a length of 12 cm and average diameter of 6 mm, were placed in 53 cm3 plastic tubes with the commercial substrate Tropstrato HP® and intermittent misting. After 90 days, the survival, mortality, cuttings with new shoots, number and length of the three main roots were evaluated. The percentages of rooting reached an average of 67.5% for P. arboreum, 51.6% for P. cernuum and 50.4% for P. diospyrifolium. A positive effect in the treatments containing the plant regulator was observed for rooting development, percentage of cuttings with shoots and number of roots per propagule, but there were variations in the responses of each species. Leaf retention was an important factor for the adventitious rooting formation in all evaluated species.

scholarly journals Determining the Rooting Ability of Hardwood Cuttings of Blueberry (Vaccinium corymbosum L.) cv. ‘Bluecrop’ Stored in Refrigerating Conditions

2020 ◽  
Vol 8 (3) ◽  
pp. 81
Author(s):  
Sabri Braha ◽  
Petrit Rama ◽  
Agim Zajmi
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Adventitious Roots ◽  
Vaccinium Corymbosum ◽  
Mother Plant ◽  
Winter Period ◽  
Naphthalene Acetic Acid ◽  
Intact Control ◽  
Rooting Ability ◽  
Time Periods

This experiment has been carried out in order to determine the rooting ability of hardwood cuttings of the blueberry cultivar ‘Bluecrop’ collected during the deep dormant winter period in the last part of January. In order to induce the adventitious roots formation, the cuttings were stimulated using Indol Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA) in various concentrations (1500, 3000, 4500 ppm), whilst one row in each box remained intact (control).  The collection of cuttings off the mother plant at the beginning of January enable the nutrients and other matter that help the process of rooting not to move from the base of cutting towards the top and consequently they remain at the base of cutting which results with quite a high rooting percentage (up to 72.5%) compared to the cuttings collected in February with rooting percentage of 67.5% and in March (52%). In both types of substrate, turf and turf-perlite 2:1 and in different time periods when cuttings have been collected, IBA proved to be better in inducing adventitious roots compared to NAA.

Vegetative propagation of Litsea monopetala, a wild tropical medicinal plant: Effects of indole-3-butyric acid (IBA) on stem cuttings

2011 ◽  
Vol 22 (3) ◽  
pp. 409-416 ◽  
Author(s):  
Tarit Kumar Baul ◽  
Mohammad Mosharraf Hossain ◽  
Mohammad Mezbahuddin ◽  
Mohammed Mohiuddin
Keyword(s):  
Medicinal Plant ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Stem Cuttings ◽  
Plant Effects

An improved culture medium for growing the orchid Cypripedium reginae axenically

1982 ◽  
Vol 60 (12) ◽  
pp. 2547-2555 ◽  
Author(s):  
Gaëtan Harvais
Keyword(s):  
Acetic Acid ◽  
Membrane Filtration ◽  
Ammonium Citrate ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Growth And Survival ◽  
Murashige Skoog ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Germination And Growth

A new medium for growing Cypripedium reginae Walt. axenically from seed was designed. Liquid culture proved unsuitable, hence a 1% agar medium supplemented with 5% potato extract was used to investigate optimal mineral element, vitamin, amino acid, sugar, and growth regulator supplements for germination, and subsequent growth. A modified Pfeffer solution with 1400 mg/L NH4NO3 + 19 mg/L ammonium citrate + 2% dextrose + 10 mg/L niacin + 5 mg/L calcium pantothenate + 5 mg/L thiamine HCl + 1 mg/L kinetin + 0.1 mg/L α-naphthaleneacetic acid gave best germination and growth to 2 years with little or no phenolic production. Gamborg's B5 medium and Murashige–Skoog (MS) medium were less than optimal when tested against the above medium. Growth regulators were more active when sterilized by membrane filtration instead of autoclaving. Of the three aminopurines tested, kinetin, benzylaminopurine (BAP), and 6(γ,γ-dimethylallylamino) purine (γγ), the order of activity was initially γγ → BAP → kinetin, but kinetin produced better greening of protocorms and plantlets, and eventually greater survival. Hence, it was chosen for further study. The auxins indole-3-acetic acid (IAA), naphthalene acetic acid (NAA), indole-3-butyric acid (IBA), and 2,4-dichlorophenoxyacetic acid (2,4-D) were also tested alone and in combination with the aminopurines. They did not stimulate germination, but improved growth and survival when combined with aminopurines. The most active of the auxins were NAA → IAA → IBA → 2,4-D. A kinetin:NAA ratio of 10:1 was very satisfactory.

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