scholarly journals Impact of Auxins on Vegetative Propagation through Stem Cuttings of Couroupita guianensis Aubl.: A Conservation Approach

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Acetic Acid ◽  
Vegetative Propagation ◽  
Adventitious Shoots ◽  
Conservation Strategy ◽  
Soil Conditions ◽  
Natural Soil ◽  
Naphthalene Acetic Acid ◽  
Stem Cuttings ◽  
Medicinal Tree ◽  
Couroupita Guianensis

The present study explores the potential of exogenous auxins in the development of adventitious shoots and roots from shoot cuttings of Couroupita guianensis (Nagalingam), a threatened tree. Experiments were conducted to assess the effect of various concentrations of auxins on shoot and root morphological traits of stem cuttings in the greenhouse. Amongst the auxins tested, significant effects on number of shoot buds’ induction and their growth were observed with α-Naphthalene Acetic Acid (NAA) treated nodal cuttings. Cent percentage of the stem cuttings of C. guianensis were rooted and shoots were induced when pretreated with 400 mg L−1 NAA for 5 min. Maximum 79% of stem cuttings responded to pretreatment of 300 mg L−1 indole-3-butyric acid (IBA) for 5 min, and 75% of stem cuttings induced shoots with 400 mg L−1 indole-3-acetic acid (IAA). Presence of at least 5 nodes on stem cuttings was found to be prerequisite for root and shoot induction. About 92% of plants were survived under natural soil conditions raised from the stem cuttings. This is the first report of vegetative propagation of C. guianensis through stem cuttings which could be used for conservation strategy and sustainable utilization of this threatened medicinal tree.

scholarly journals Studies on Hormonal Effects on Rooting of Marcotting and Stem-Cuttings of Akee Apple (Blighia sapida K. D. Koenig)

2016 ◽  
Vol 8 (4) ◽  
pp. 468-471 ◽  
Author(s):  
Ehoniyotan Olayemi IBUKUN
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Callus Formation ◽  
Hormonal Treatment ◽  
The Other ◽  
Naphthalene Acetic Acid ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Hormonal Treatments

The effect of hormone on the rooting of stem-cuttings and marcotting of akee apple was studied using a combination between Indole -3- Butyric Acid (IBA) and 1- Naphthalene Acetic Acid (NAA). Stem-cuttings from mature akee trees from Challenge, Jalala and Ganmo in Ilorin, were treated with different dilutions of the liquid hormone in the combination of 1.0% Indole-3- butyric acid + 0.5% 1-Naphthaleneacetic acid before propagating them in a non-mist propagator. Marcotting was also carried-out on trees, using the hormonal combination of different dilutions. Observations and the results obtained revealed that the hormonal combination had significant effect on the rooting of stem-cuttings and marcotting. Both marcotting and stem cuttings did not produce at the end of the experiment roots in the absence of the hormonal treatment; a particular aspect was marcotting that initials produced roots. On the other hand, both marcotting and stem cuttings produced roots with the hormonal treatments; more roots were produced using the combination of 2,000 ppm of IBA and 1,000 ppm of NAA, compared with lower concentrations of the hormone mixtures. After callus formation, 2,000 ppm of IBA and 1,000 ppm of NAA combination gave the best results within stem-cuttings. Based on the results obtained, it was concluded that the combination of IBA and NAA in appropriate concentration promoted rooting in Akee apple and therefore are highly valuable for the vegetative propagation of this species through stem cutting and marcotting.

scholarly journals Vegetative propagation of Garcinia lucida Vesque (Clusiaceae) using leafy stem cuttings and grafting

Afrika Focus ◽  
2017 ◽  
Vol 27 (3) ◽  
Author(s):  
Bertin Takoutsing ◽  
Alain Tsobeng ◽  
Zacharie Tchoundjeu ◽  
Ann Degrande ◽  
Ebenazar Asaah
Keyword(s):  
Acetic Acid ◽  
Leaf Area ◽  
Vegetative Propagation ◽  
Naphthalene Acetic Acid ◽  
High Porosity ◽  
Stem Cuttings ◽  
Forest Zone ◽  
Indole Butyric Acid ◽  
Humid Forest ◽  
Humid Forest Zone

Garcinia lucida Vesque (Clusiaceae) is a tree species that is highly valued for its medicinal properties by rural households in the humid forest zone of Cameroon. However, the unsustainable exploitation of the species threatens its long-term regeneration. This study focuses on its vegetative propagation via stem cuttings in non-mist propagators and through grafting. The study tests the effects of three rooting media (sand, sawdust, sand + sawdust (1/1); three leaf sizes (0,25 and 50 cm2); and three types of hormone [indole butyric acid (IBA), indole-3-acetic acid (IAA) and naphthalene acetic acid (NAA)], applied as a single dose. Furthermore, three grafting techniques (cleft, side tongue and whip-and-tongue grafting) were tested. All experiments were designed as completely randomized blocks with three replicates. Results showed that cuttings require a medium that has low water holding capacity and high porosity, and a leaf area of 50 cm2 and NAA treatment; grafting success was affected by the technique used, with top cleft grafting yielding a 100% success rate. From this preliminary study, it is concluded that G. lucida is amenable to vegetative propagation by cuttings and grafting. Key words: tree domestication, leaf area, non-mist propagator, rooting hormone, rooting medium, under-utilised species 

scholarly journals In vitro culture of rose species (Rosa spp.) via axillary bud growth

2007 ◽  
pp. 1-13
Author(s):  
Zosimo de la Rosa ◽  
Marilyn Belarmino
Keyword(s):  
Acetic Acid ◽  
Adventitious Shoots ◽  
Axillary Buds ◽  
Plant Production ◽  
Axillary Bud ◽  
Naphthalene Acetic Acid ◽  
Stem Cuttings ◽  
Indole Butyric Acid ◽  
Single Shoot

The utilization of nodal stem cuttings containing dormant axillary buds as explants for plant production of two rose species; Rosa chinensis cv.‘Old Blush’ and R. centifolia cv. ‘Petite de Hollande’ was demonstrated in this study. This propagation technique required the breaking of dormant axillary buds by aseptically culturing them in agarsolidified Woody Plant Medium (WPM) added with 0.5, 1.0, and 2.0 mg l-1 of 6benzylaminopurine (BAP) or, a combination of 2.0 mg l-1 BAP and 0.01 mg l-1 naphthalene acetic acid (NAA). Production of multiple adventitious shoots from one nodal stem explant was obtained after three months of culture in medium supplemented with 1.0 or 2.0 mg l-1 BAP. Four types of plant morphology; single shoot (type 1), multiple shoots with normal leaves (type 2), cluster of tiny shoots with curly leaves (type 3), and single shoot with callus at the base (type 4) were observed from the axillary bud-derived plantlets. The rooting of plantlets was induced in WPM containing 0.25 to 1.0 mg l-1 of indole-butyric acid (IBA) or, 2.0 mg l-1 of indole-3-acetic acid (IAA).

scholarly journals VEGETATIVE PROPAGATION OF Spondias tuberosa E Spondias dulcis WITH THE USE OF IMMERSION IN INDOLE ACETIC ACID

2019 ◽  
Vol 32 (4) ◽  
pp. 858-866
Author(s):  
GABRIELA TEODORO ROCHA ◽  
AYURE GOMES DA SILVA ◽  
JULIANA BEZERRA MARTINS ◽  
NEI PEIXOTO ◽  
FABRICIO RODRIGUES
Keyword(s):  
Acetic Acid ◽  
Vegetative Propagation ◽  
Indole Acetic Acid ◽  
Stem Cuttings ◽  
Randomized Design ◽  
Spondias Tuberosa ◽  
Completely Randomized Design ◽  
Low Efficiency ◽  
Indole 3 Acetic Acid ◽  
Total Fresh Weight

ABSTRACT The objective of this work was to evaluate the effect of six indole-3-acetic acid (IAA) concentrations and three immersion times in IAA solutions on the vegetative propagation of Spondias dulcis and Spondias tuberosa by stem cuttings. The experiment was conducted in a greenhouse, using a completely randomized design, with a 6×3 factorial arrangement consisting of six indole-3-acetic acid concentrations (0, 2, 4, 6, 8, and 10 g L-1) and three immersion times (8, 16, and 24 seconds), with ten cuttings of S. dulcis and S. tuberosa per plot, and three replications. Percentage of surviving cuttings, and percentage of rooted cuttings, number of leaf buds, number of roots per cutting, root length, and total fresh weight were evaluated at 180 days after planting. Data were subjected to analysis of variance by the F test and to regression analysis. Vegetative propagation from woody cuttings of S. dulcis is not viable to produce seedlings, thus, new studies on this technique for this species are needed. Vegetative propagation from woody cuttings of S. tuberosa is satisfactory, but with low efficiency for improvement and emergence of new shoots and roots; it is more effective when using a concentration of 10 g L-1 of indole-3-acetic acid and immersion time in the solution of 16 seconds.

scholarly journals Factors Affecting Vegetative Propagation of Wintersweet (Chimonanthus praecox) by Softwood Cuttings

HortScience ◽  
2020 ◽  
Vol 55 (11) ◽  
pp. 1853-1860
Author(s):  
Huan Hu ◽  
Nan Chai ◽  
Haoxiang Zhu ◽  
Rui Li ◽  
Renwei Huang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Vegetative Propagation ◽  
Design Method ◽  
Orthogonal Design ◽  
Naphthalene Acetic Acid ◽  
Hormone Concentration ◽  
Range Analysis ◽  
Indole Butyric Acid ◽  
Factors Affecting ◽  
Chimonanthus Praecox

Wintersweet (Chimonanthus praecox) is one of the most popular winter-blooming species. Effective vegetative propagation is necessary for commercial usage and protection of wintersweet. In the current study, the four factors, namely hormone type (A), hormone concentration (B), soaking duration (C), and medium (D), were assessed using an L16 (44) orthogonal test design. The hormone types include ABT (A1), α-naphthalene acetic acid (NAA) (A2), indole butyric acid (IBA) (A3), and indole-3-acetic acid (IAA) (A4); the hormone concentrations include 100 mg·L−1 (B1), 500 mg·L−1 (B2), 1000 mg·L−1 (B3), and 1500 mg·L−1 (B4); the soaking durations include 5 seconds (C1), 5 minutes (C2), 30 minutes (C3), and 3 hours (C4); and the mediums include perlite: peat in the ratios 1:0 (D1), 2:1 (D2), 1:1 (D3), and 1:2 (D4). The results showed that hormone and proper medium could significantly improve the cutting survival, rooting, and sprouting, whereas poor factor combinations, especially high hormone concentrations combined with long soaking durations may be threatened to the cuttings and rooting. In actual experiments, we successfully obtained an excellent rooting percentage (62.22%) of wintersweet from treatment No. 5 (A2B1C2D3), which is perlite and peat (1:1) as the medium and soaking the cuttings in 100 mg·L−1 NAA for 5 minutes as the hormone treatment. This combination can already meet the requirements for commercial production. A range analysis showed that the medium and hormone concentration were the most important factors affecting the cutting of wintersweet. An analysis of variance also showed that the medium and hormone concentration can significantly or extremely significantly affect most cutting indicators. Moreover, our results revealed that an orthogonal design method is an effective tool for establishing an improved technique for cutting propagation.

scholarly journals Somatic embryogenesis and plant regeneration in purple food yam (Dioscorea alata L.)

2008 ◽  
pp. 22-33 ◽  
Author(s):  
Marilyn Belarmino ◽  
Jocelyn Gonzales
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Adventitious Shoots ◽  
Pantothenic Acid ◽  
Naphthalene Acetic Acid ◽  
Dioscorea Alata ◽  
Ms Medium ◽  
Leaf Petiole

A study was conducted to establish a reliable procedure for somatic embryogenesis and plant regeneration from callus cultures of purple food yam (Dioscorea alata L.). The procedure involved three steps; (1) culture of nodal stem segments from greenhousegrown plants to generate in vitro plantlets; (2) induction of callus from the leaf, petiole and nodal stem tissues; and (3) initiation of somatic embryo from callus. Results showed that the agar-solidified Murashige and Skoog (MS) medium containing 30 gl-1 sugar, 0.1 gl-1 α-cysteine , 10 mgl-1 calcium pantothenic acid, 2.0 mgl-1 asparagine, 2.0 mgl-1 arginine, 80.0 mgl-1 adenine sulfate (AdSO4) and 0.1 mgl-1 naphthalene acetic acid (NAA) effectively broke dormancy of lateral buds of nodal stem cultures from both ‘VU-2’ and ‘Kinampay‘ varieties. Production of multiple adventitious shoots occurred after transfer of in vitro nodal pieces to the same medium added with 1.0 mgl-1 benzylamino purine (BAP) or, MSA medium. Callus was effectively induced from the vegetative tissues in MS medium added with 1.0 mgl-1 2,4-Dichlorophenoxy acetic acid (2,4-D) or, with picloram. Among the three types of explants, the nodal stem was the most suitable which produced purplish nodular embryogenic callus. A higher percentage of nodal stem-derived calli produced globular embryos in MS medium containing 1.0 mgl-1 2,4-D and 0.5 mgl-1 BAP, or in 1.0 mgl-1 picloram and 0.5 mgl-1 BAP than, in the plant growth regulator-free medium (control). The maturation of embryos was facilitated by one-month culture in MS medium containing 0.1 mgl-1 ABA and 100 mgl-1 glutamine. This step improved the germination of somatic embryos in one-half strength PGR-free MS medium containing 100 mgl-1 glutamine (regeneration medium). All somatic embryoderived plantlets were morphologically normal and established well in soil.

scholarly journals Effect of naphthalene acetic acid on sprouting and rooting of stem cutting in Mussaenda species

2019 ◽  
Vol 30 (1) ◽  
pp. 48-54
Author(s):  
MHA Rashid ◽  
A Nahar
Keyword(s):  
Acetic Acid ◽  
Root Formation ◽  
Block Design ◽  
Stem Cutting ◽  
Naphthalene Acetic Acid ◽  
Combined Effects ◽  
Stem Cuttings ◽  
Treatment Combination ◽  
Red Flag ◽  
Number Of Leaves

Mussaendas are increasingly popular for the showy colour they provide during much of the year in garden landscapes. However, root formation in stem cuttings is a major challenge for cultivation of Mussaenda species. Therefore, the experiment was carried out at the Landscaping section of the Department of Horticulture, Bangladesh Agricultural University, Mymensingh during the period from August to November 2018 to study the effect of phytohormone naphthalene acetic acid (NAA) on sprouting and rooting of stem cuttings in Mussaenda species. The two-factor experiment consisted of three Mussaenda species viz., Mussaenda frondosa (White flag bush), Mussaenda philippica (Pink flag bush) and Mussaenda erythrophylla (Red flag bush), and five concentrations of NAA viz., 0 (control), 0.1%, 0.2%, 0.3% and 0.4%. The experiment was laid out in randomised complete block design (RCBD) with three replications. The results revealed that all the traits related to sprouting and rooting potential of stem cuttings were significantly influenced by combined effects of Mussaenda species and various NAA concentrations. The maximum sprouted bud per cutting (3.57), highest number of roots per cutting (5.88) and the longest root length (4.32 cm) were recorded from M. philippica except number of leaves per stem cutting (4.42) in M. erythrophylla. The increasing concentrations of NAA application responded positively on sprouting and rooting characters in all three Mussaenda species used for this experiment. The best results obtained from the treatment where NAA was applied at the concentration of 0.3% with 3.92 sprouted buds per cutting, 4.77 leaves per cutting, 7.15 roots per cutting and 5.26 cm length of roots. The treatment combination of M. philippica (Pink flag bush) and 0.3% NAA, followed by NAA at 03% with M. erythrophylla (Red flag bush) was found to be best in terms of sprouting and rooting of stem cuttings in Mussaenda. Progressive Agriculture 30 (1): 48-54, 2019

scholarly journals PENGARUH LAMA PERENDAMAN PANGKAL SETEK DALAM LARUTAN NAA (NAPTHALENE ACETIC ACID) PADA PERTUMBUHAN SETEK LADA

2020 ◽  
Vol 8 (2) ◽  
pp. 311
Author(s):  
Syaicha Fachrun Nisa ◽  
Niar Nurmauli ◽  
Akari Edy ◽  
Sugiatno Sugiatno
Keyword(s):  
Acetic Acid ◽  
Dry Weight ◽  
Piper Nigrum ◽  
Naphthalene Acetic Acid ◽  
Stem Cuttings ◽  
Fresh Weight ◽  
Orthogonal Comparison ◽  
Napthaleneacetic Acid ◽  
Α Level ◽  
Total Fresh Weight

Effect of soaking duration on base stem cuttings in NAA solution (Naphthalene Acetic Acid) on the growth of pepper The need for pepper is increasing along with the development of the food industry with the basic ingredients of pepper. The determining factor of the quality of pepper produced comes from the seeds used. This study aims to determine the effect of soaking DURATION on pepper cuttings (Piper nigrum L.) in the Napthaleneacetic Acid (NAA) solution. The study was conducted in October 2018 until February 2019 in the greenhouse and Plant Science Laboratory of the Faculty of Agriculture, University of Lampung, Bandar Lampung. This study uses a completely randomized group design (RKTS). The treatment of immersion base cuttings in NAA (N) solution concentrates 500 ppm consisting of : without NAA (n0), immersion 0 minutes (n1), soaked 15 minutes (n2), soaked 30 minutes (n3), soaked 60 minutes (n4), and soaked 120 minutes (n5). Homogeneity of variability between treatments was tested by Bartlet Test and data additivity was tested by Tukey test, then continued with Variance Analysis (ANARA). Comparison of the middle value with the orthogonal comparison test at α level of 5%. The results showed that: (1) giving NAA affected the number of roots in the book, the fresh weight of the roots in the book, the dry weight of the roots in the book, the total number of roots, and the total fresh weight of the roots; (2) administration of NAA by immersion is better than dipping in the number of roots in the book, the fresh weight of the roots in the book, the dry weight of the roots in the book, the total number of roots, and the total fresh weight of the roots; and (3) length of soaking cuttings in NAA solution has no effect on the growth of pepper cuttings.

scholarly journals In Vitro Propagation of Camellia oleifera Abel. Using Hypocotyl, Cotyledonary Node, and Radicle Explants

HortScience ◽  
2016 ◽  
Vol 51 (4) ◽  
pp. 416-421 ◽  
Author(s):  
Ze Li ◽  
Xiaofeng Tan ◽  
Zhiming Liu ◽  
Qing Lin ◽  
Lin Zhang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Adventitious Shoots ◽  
Shoot Multiplication ◽  
Cotyledonary Node ◽  
Direct Organogenesis ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Camellia Oleifera ◽  
Cotyledonary Nodes

Camellia oleifera Abel. is one of four major woody oil plants in the world. The objective of the current study was to evaluate the effect of different plant growth regulators (PGRs) and concentrations on direct organogenesis using cotyledonary nodes, hypocotyls, and radicle explants. High induction frequency of adventitious shoots were obtained from cotyledonary nodes, hypocotyls, and radicle explants (85.2%, 73.6%, and 41.0%, respectively) when cultured on half-strength Murashige and Skoog (1/2 MS) medium containing 2.0 mg·L−1 6-benzylaminopurine (BA) and 0.1 mg·L−1 indole-3-acetic acid (IAA). Microshoots from cotyledonary nodes, hypocotyls, and radicle explants were then transferred to 1/2 MS medium containing 2.0 mg·L−1 BA and 0.05 mg·L−1 indole-3-butyric acid (IBA) for shoot multiplication, resulting in 6.9 shoots per explant. The shoots were transferred to Woody Plant Medium (WPM) supplemented with various α-naphthalene acetic acid (NAA) and gibberellic acid (GA3) for shoot elongation. The mean length of shoots and the number of leaves per shoot were 3.7 and 6.6 cm, respectively, in WPM supplemented with 0.5 mg·L−1 NAA and 3.0 mg·L−1 GA3. The highest rooting of shoots (90.2%) or the number of roots per shoot (7.2) was obtained when elongated microshoots were transferred to 1/2 MS medium supplemented with 3.5% perlite, 1.0 mg·L−1 IBA and 2.0 mg·L−1 NAA. The rooted plantlets were successfully acclimatized in the greenhouse with a survival rate of 90.0%. The in vitro plant regeneration procedure described in this study is beneficial for mass propagation and improvement of C. oleifera through genetic engineering.

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