scholarly journals Androgenesis Induced in Nicotiana alata and the Effect of Gamma Irradiation

2015 ◽  
Vol 7 (1) ◽  
pp. 66-71
Author(s):  
Ayman EL-FIKI ◽  
Gamal EL-METABTEB ◽  
Abdel-Hadi SAYED ◽  
Mohamed ADLY
Keyword(s):  
Radiation Dose ◽  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Soluble Proteins ◽  
Total Soluble Protein ◽  
Nicotiana Alata ◽  
Ms Medium ◽  
Anther Length ◽  
Gamma Radiation Dose ◽  
Haploid Plants

Nicotiana alata anthers cultured on different modified media based on MS, MT and N were used to obtain haploid plants through direct and indirect ways. The haploid plants resulting on MS medium ranged from 52% - 80%, on MT medium ranged from 32% - 52% and on N medium ranged from 28% - 44%. Accordingly, the best medium used for haploid induction was MS supplemented with 0.2 mgl-l NAA + 0.5 mgl-l KIN. On the other hand, MS medium supplemented with 0.4 mgl-l NAA + 0.5 mgl-l KIN or 1.0 mgl-l BAP + 0.5 mgl-l NAA were the best mediums for callus induction and plant regeneration, respectively. Morphologically, the leaf size, stem highest and diameter, flower size and diameter, anther length and number were about 67% of the diploid plants growth. Irradiated anthers with doses of 0, 2.5, 5, 7.5, 10, 15, 20 and 25 Gy caused reducing the number of haploid plants with increasing gamma radiation dose. For the haploid plants irradiated with same doses, the mortality percentage of bud survival was increasing with increasing gamma radiation dose. The irradiated callus with doses of 0, 5, 10, 15 and 20 Gy was affected negatively on growth rate and morphology. Proline content in irradiated plantlets increased with increasing gamma radiation dose. As well, total soluble protein content was increased with gamma irradiation up to 10 Gy. However, the higher doses caused a severe decrease of total soluble proteins. The production of proline and total soluble proteins in haploid plants were 48.6% and 69.5%, respectively comparing with diploid plants.

scholarly journals Genetic Diversity in Haploid Nicotiana alata Induced by Gamma Irradiation, Salt Tolerance and Detection of These Differences by RAPD

2016 ◽  
Vol 8 (1) ◽  
pp. 73-80 ◽  
Author(s):  
Ayman EL-FIKI ◽  
Abdel-Hadi SAYED ◽  
Gamal EL-METABTEB ◽  
Mohamed ADLY
Keyword(s):  
Gamma Radiation ◽  
Negative Impact ◽  
Random Amplified Polymorphic Dna ◽  
Combined Effect ◽  
Total Soluble Protein ◽  
Polymorphic Band ◽  
Radiation Doses ◽  
Nicotiana Alata ◽  
Ms Medium ◽  
Band Number

Haploid plants of Nicotiana alata were cultured in vitro on MS medium with IAA + KIN. The resulting plantlets were irradiated using gamma radiation doses of 10, 15, 20 and 25 Gy. Single node pieces were cut and transferred onto fresh MS medium. Gamma radiation doses caused the death of 9% and up to 28% of explants. NaCl concentrations caused the death of 8% up to 36% of explants, while the combined effect between gamma radiation doses and salinity had an impact suffused on the percentage of survival. The combined effect of gamma radiation doses 20 Gy and 25 Gy on NaCl concentrations of 100, 150 and 200 mM were deadly. Even more, the combined effect of gamma radiation doses and salinity had a severe negative impact on both the proline content and total soluble protein. Random amplified polymorphic DNA (RAPD) analysis was used to determine the degree of genetic variation in treated haploid Nicotiana alata plants. Total genomic DNAs from different haploid plantlets treated were amplified using five arbitrary primers. Two hundred and seventy bands were detected from plantlets irradiated with doses of 15, 20 and 25 Gy, with polymorphic band number 226 (83.7%). The total number of bands resulted from plant grew on 150 mM and 200 mM NaCl were 260 bands with polymorphic bands 185 (85.6%). However, the total number of bands produced from combined effects between gamma rays and salinity (20 Gy X 50 mM NaCl, 20 Gy X 100 mM NaCl and 25 Gy X 50 mM NaCl) were 270, with polymorphic band number 231 (85.5%). High similarity between treatments was revealed. Treatments relationships were estimated through cluster analysis (UPGMA) based on RAPD data.

Optimization of gamma radiation dose for induction of mutations in okra

2021 ◽  
pp. 1-11
Author(s):  
Soham Hazra ◽  
Shouvik Gorai ◽  
V Umesh Kumar ◽  
Sudip Bhattacharya ◽  
Anirban Maji ◽  
...  
Keyword(s):  
Radiation Dose ◽  
Gamma Radiation ◽  
Gamma Radiation Dose

scholarly journals Terrestrial Gamma Radiation Dose Rate of West Sarawak

2017 ◽  
Vol 156 ◽  
pp. 00006
Author(s):  
A. Izham ◽  
A.T. Ramli ◽  
W.M. Saridan Wan Hassan ◽  
H.N. Idris ◽  
N.A. Basri
Keyword(s):  
Radiation Dose ◽  
Gamma Radiation ◽  
Dose Rate ◽  
Radiation Dose Rate ◽  
Gamma Radiation Dose

Terrestrial Gamma Radiation Dose in Hong Kong

1992 ◽  
Vol 62 (1) ◽  
pp. 77-81 ◽  
Author(s):  
Man-Yin W. Tso ◽  
Chung-Chum Li
Keyword(s):  
Hong Kong ◽  
Radiation Dose ◽  
Gamma Radiation ◽  
Gamma Radiation Dose

The rain induced gamma-radiation dose rate enhancement at Järvselja SMEAR station

2021 ◽  
Author(s):  
Urmas Hõrrak ◽  
Xuemeng Chen ◽  
Kristo Hõrrak ◽  
Uko Rand ◽  
Kaupo Komsaare ◽  
...  
Keyword(s):  
Radiation Dose ◽  
Gamma Radiation ◽  
Dose Rate ◽  
Atmospheric Aerosol ◽  
Aerosol Particles ◽  
Aerosol Formation ◽  
Radiation Dose Rate ◽  
Air Mass ◽  
Rate Enhancement ◽  
Gamma Radiation Dose

<p>The SMEAR Estonia station (58.277663 N, 27.308266 E, 36 m a.s.l.) was established in south-east of Estonia at the Järvselja Experimental Forestry in 2012 to investigate the atmosphere-biosphere interactions and atmospheric aerosol formation and growth.</p><p>In summer 2019, the gamma-radiation monitor GammaTRACER XL2-3 (Saphymo GmbH) was set up at Järvselja station and the rain sensor DRD11A (Vaisala Oyj) in autumn 2019. These devices enable to measure the gamma-radiation dose rate and precipitation intensity, which affect the ionization rate of atmospheric air close to ground, with high accuracy and time resolution, and complement our measurement system of atmospheric ions and aerosol particles.</p><p>The gamma-radiation dose rate measurements at about 1.2 m above the ground reveled on relatively steady background about 70 nSv/h occasional events with increase up to about 110 nSv/h, which correlated well with rainfall intensity. Commonly such events last 3-4 hours, but in specific meteorological situation with continuous long-lasting rain and air mass movement from southerly directions the effect can last 2-3 days, resulting in gradual increase in gamma-radiation dose rate level during about 24 h.</p><p>Such a phenomenon is known to occur due to wet deposition of radioactive aerosol particles during rain, namely due to the radon (<sup>222</sup> Rn) short-lived daughter progeny products (Po-218, Pb-214, Bi-214) attached to atmospheric aerosol particles. The radon (<sup>222</sup> Rn) daughter progeny involvement is confirmed by simultaneous gamma-spectrometric measurements with SARA AGS711F (Envinet GmbH) at Tõravere station (58° 15' 52,9" N, 26° 27' 42,1", 72 m), located about 50.3 km west from the Järvselja SMEAR station. The gamma dose rates showed very similar temporal behavior when both stations were affected by the same air mass with precipitation zone passing over the stations.</p><p>To our best knowledge, the details of rain-induced enhancement of gamma-radiation dose rate and atmospheric processes behind the phenomenon are not well known and are worth future investigations. The events of rain induced gamma-radiation dose rate enhancement at Järvselja SMEAR and Tõravere station are analyzed and discussed in more detail in the presentation and the spatial representativity of the phenomenon is estimated based on the gamma-radiation monitoring network data of Estonian Early Warning System.</p>

scholarly journals Spatial distribution of gamma radiation dose rates from natural radionuclides and its radiological hazards in sediments along river Iju, Ogun state Nigeria

MethodsX ◽  
2020 ◽  
Vol 7 ◽  
pp. 101086
Author(s):  
Omeje Maxwell ◽  
Adewoyin Olusegun O. ◽  
Joel Emmanuel S. ◽  
Ikechukwu Ijeh B. ◽  
Omeje Uchechukwu A. ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
Radiation Dose ◽  
Gamma Radiation ◽  
Natural Radionuclides ◽  
Dose Rates ◽  
Ogun State ◽  
Radiological Hazards ◽  
Gamma Radiation Dose
Sign in / Sign up

Export Citation Format

Share Document