scholarly journals Production of Itaconic Acid from Jatropha curcas Seed Cake by Aspergillus terreus

2013 ◽  
Vol 5 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Amina M. AHMED EL-IMAM ◽  
Muinat O. KAZEEM ◽  
Mutiat B. ODEBISI ◽  
Mushaffa A. OKE ◽  
Azeezat O. ABIDOYE
Keyword(s):  
Itaconic Acid ◽  
Jatropha Curcas ◽  
Substrate Concentration ◽  
Aspergillus Terreus ◽  
Maximum Yield ◽  
Inoculum Size ◽  
Daily Maximum ◽  
Dilute Acid Hydrolysis ◽  
Seed Cake ◽  
Jatropha Seed

Submerged substrate fermentation of Jatropha seed cake, a by-product of oil extraction from Jatropha curcas seed was carried out using Aspergillus terreus for the production of itaconic acid. The Jatropha seed cake was initially converted into fermentable sugars by dilute acid hydrolysis using 50% sulphuric acid. The rate of hydrolysis was 1.04 gL-1. The fermentation process was carried out at room temperature, agitation of 400 rpm and three physico-chemical parameters (pH, inoculum size and substrate concentration) were varied. Itaconic acid and glucose assays were carried out by spectrophotometry and Dinitrosalicylic acid methods respectively daily. Maximum yield of itaconic acid was 48.70 gL-1 at 5 ml of inoculum size, 50 g substrate concentration and pH 1.5. The residual glucose concentration increased for the first two days of fermentation after which it began to decrease as the itaconic acid concentration increased. The least concentration of itaconic acid observed was 6.00 gL-1, obtained after 24 hours of fermentation with 4 ml inoculum size, 50 g substrate concentration and at pH 1.5. The findings of this work indicate that Jatropha curcas seed cake is a suitable substrate for itaconic acid production.

scholarly journals Fermentatative production of itaconic acid by Aspergillus terreus using Jatropha seed cake

2007 ◽  
Vol 6 (18) ◽  
pp. 2140-2142 ◽  
Author(s):  
MuralidharaRao Dowlathabad ◽  
M D Jaheer Hussain S ◽  
P V ◽  
Rangadu u ◽  
Subramanyam K ◽  
...  
Keyword(s):  
Itaconic Acid ◽  
Aspergillus Terreus ◽  
Seed Cake ◽  
Jatropha Seed

scholarly journals In-situ Alkaline Transesterification of Jatropha curcas seed Oil for Production of Biodiesel and Nontoxic Jatropha seed Cake

2014 ◽  
Vol 4 (1) ◽  
pp. 7
Author(s):  
Novizar Nazir ◽  
Djumali Mangunwidjaja ◽  
Dwi Setyaningsih ◽  
Sri Yuliani ◽  
Mohd. Ambar Yarmo ◽  
...  
Keyword(s):  
Jatropha Curcas ◽  
Seed Oil ◽  
Seed Cake ◽  
Jatropha Seed

scholarly journals Characterization of Aspergillus Niger 65i6 lipase from solid-state fermentation using Jatropha seed cake medium

2017 ◽  
Vol 20 (2) ◽  
pp. 108 ◽  
Author(s):  
Chusnul Hidayat ◽  
Sari Darmasiwi ◽  
Maulina Nurikasari ◽  
Muhammad Nur Cahyanto
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Jatropha Curcas ◽  
Enzyme Stability ◽  
Water Concentration ◽  
Lipase Production ◽  
Seed Cake ◽  
Effects Of Ph ◽  
Jatropha Seed

Jatropha curcas seed cake contains a high amount of protein, and consequently has very high potentialas a medium for lipase production. The objective of this research was to characterize lipase from Aspergillusniger 6516, which was produced by solid-state fermentation on Jatropha curcas seed cake as the medium. The effects of pH and temperature on enzyme activity were evaluated, along with substrate specifcity and enzyme stability. Fermentation was performed at a water concentration of 63% and temperature of 30 °C for 7 days. The results showed that the optimum pH and temperature for Aspergillus niger 6516 lipase activities were 8.0 and 40 °C, respectively. The lipase had the substrate specifcity to hydrolyze long-chain fatty acids and was stable in polar organic solvents. The lipase had a molecular weight, Km and vmax about 19 kDa, 0.27 µmol/ml, and 52.63 µmol/ml/min, respectively. The results also suggested that the produced lipase from Aspergillus niger 6516 was an alkaline lipase. Based on these results, we conclude that Jatropha seed cake is a suitable medium for lipase production.

scholarly journals Bioethanol Production from Stalk Residues of Chiquere and Gebabe Varieties of Sweet Sorghum

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Nitin Mahendra Chauhan ◽  
Sunil Tulshiram Hajare ◽  
Buzuayehu Mamo ◽  
Abreham Assefa Madebo
Keyword(s):  
Dilution Rate ◽  
Sweet Sorghum ◽  
Agricultural Sector ◽  
Negative Impact ◽  
Maximum Yield ◽  
Value Added ◽  
Inoculum Size ◽  
Bioethanol Production ◽  
Dilute Acid Hydrolysis ◽  
Plant Materials

Bioethanol produced from renewable resource has potential to solve environmental pollution and to satisfy the need of demand and supply. It favours the use of nonfood lignocellulosic materials. Ethanol produced from plant materials can sustain the economy by reducing cost of imported petroleum, emitting neutral CO2. Moreover, it enhances the economy by providing value added market opportunities for transportation and agricultural sector. Therefore, the objective of the study was to investigate bioethanol production from stalk residues of Chiquere and Gebabe varieties of sweet sorghum collected from West Arsi Zone, Ethiopia. Response surface methods with a three factor (inoculum size, pH, and dilution rate) with triplicate run by using the Box–Behnken method was referred. The experiment employed dilute acid hydrolysis, because it is an easy and productive process by treating the stalks with 4% of sulfuric acid for effective hydrolysis of substrate. Finally, the fermentation was carried out at 30°C for 72 hours on a shaker at 180 rpm by using Saccharomyces cerevisiae. The significance of the result was evaluated by using ANOVA, where P values <0.05 were considered statistically significant. In the process, maximum yield of ethanol was obtained at an inoculum size of 5% (22.40%), pH level of 4.0 (21%), and dilution rate at 10 ml (21.46%). Very low yeast inoculum size and dilution factor have positive effect on the yield of ethanol, whereas very high dilution rate produced negative impact on ethanol production. FTIR spectroscopy peaks associated with O-H, C-O, and C-H stretching vibrations confirmed the presence of ethanol obtained from sweet sorghum stalks. The results of our study indicated that, being available in bulky amounts and nonedible material, sweet sorghum stalks can serve as potential feedstock for bioethanol production in developing countries such as Ethiopia.

scholarly journals BIOSYNTHESIS OF ITACONIC ACID IN ASPERGILLUS TERREUS

1957 ◽  
Vol 226 (2) ◽  
pp. 689-701
Author(s):  
Ronald Bentley ◽  
Clara P. Thiessen
Keyword(s):  
Itaconic Acid ◽  
Aspergillus Terreus

scholarly journals Investigation of Co-Gasification Reactivity of Torrefied Jatropha Seed Cake with Illinois #6 Coal Char

BioResources ◽  
2016 ◽  
Vol 11 (3) ◽  
Author(s):  
Yuhong Qin ◽  
Zibing Zhao ◽  
Tomasz Wiltowski ◽  
Mashal Aloqaili ◽  
Yanna Liang
Keyword(s):  
Coal Char ◽  
Seed Cake ◽  
Gasification Reactivity ◽  
Jatropha Seed
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